Development of fluorescence-detected circular dichroism by modulated beam in the wavelength axial direction

波长轴方向调制光束荧光检测圆二色性的发展

• 批准号: 16580112
• 负责人: YAMAMOTO Atsushi
• 金额: $ 2.05万
• 依托单位: Chubu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16580112/
• 关键词: Fluorescence-detected circular dichroism; High-performance Liquid Chromatography; Fluorescence Spectrometer; Capillary electrophoresis; Borate complex; 波長軸変調型FDCD検出法; フローセル; カテキン

A novel technique is developed that permits fluorescence-detected circular dichroism (FDCD) detection in a conventional fluorescence spectrophotometer. The proposed detector consists of a polarizing prism and a retardation plate fitting on the excitation light side of the fluorescence spectrometer. But this technique was suffered from the appearance of an artifact wave on the excitation spectrum, which complicated the detection of the FDCD wave. To solve this problem, the scan speed of the spectrometer could not be helped slowing and the integration of the spectra by repetitive scans was required. It made us the stopped-flow measurement, so this technique was not suit for the real-time determination.The enantiomeric purity determination of catechins in tea drinks was performed by the proposed system. To optimize the determination conditions, the enantiomeric purity of catechin (2-(3,4-dihydroxylphenyl)-3,4-dihydro-2H-1-benzopyran-3,5,7-triol) could be analyzed without pretreatment except for the filtration of tea sample.

开发了一种新的技术，允许荧光检测圆二色性（FDCD）检测在传统的荧光分光光度计。该探测器由偏振棱镜和安装在荧光光谱仪激发光侧的延迟板组成。但是，这种技术的缺点是在激发光谱上出现伪影波，这使得FDCD波的检测变得复杂。为了解决这个问题，光谱仪的扫描速度不能被帮助减慢，并且需要通过重复扫描对光谱进行积分。该方法采用停流测量法，不适合于实时测定，利用该系统对茶饮料中儿茶素的对映体纯度进行了测定。通过对测定条件的优化，茶叶样品经过滤后，无需预处理即可测定儿茶素（2-（3，4-二羟基苯基）-3，4-二氢-2H-1-苯并吡喃-3，5，7-三醇）的对映体纯度。

项目成果

Chiral ligand exchange capillary electrophoresis using borate anion as a central ion.

• DOI: 10.1039/b406875k
• 发表时间: 2004-11
• 期刊: The Analyst
• 作者: S. Kodama;A. Yamamoto;R. Iio;K. Sakamoto;A. Matsunaga;K. Hayakawa

HPLC/fluorescence detected circular dichroism detection by three-dimensional fluorescence detector

HPLC/荧光检测 三维荧光检测器圆二色性检测

• 发表时间: 2005
• 期刊: Annual Report of College of Bioscinece and Biotechnology 4
• 作者: A.Yamamoto;S.Kodama;R.Ito;H.Nakazawa;H.Kumagai
• 通讯作者: H.Kumagai

Chiral ligand exchange micellar electro-kinetic chromatography using borate anion as a central ion

以硼酸根阴离子为中心离子的手性配体交换胶束电动色谱

• 发表时间: 2005
• 期刊: Electrophoresis 26
• 作者: S.Kodama;A.Yamamoto;R.Iio;S.Aizawa;K.Nakagomi;K.Hayakawa
• 通讯作者: K.Hayakawa

Mechanism of enantioseparation of DL-pantothenic acid in ligand exchange capillary electrophoresis using diol-borate system

二醇-硼酸盐体系配体交换毛细管电泳中 DL-泛酸对映体分离的机理

• 发表时间: 2006
• 期刊: Electrophoresis 27
• 作者: Hiroshi Ito;et al.;Sen-ichi Aizawa
• 通讯作者: Sen-ichi Aizawa

Chiral ligand, exchange micellar electrokinetic chromatography using borate anion as a central ion

手性配体，以硼酸根阴离子为中心离子的交换胶束电动色谱

• 发表时间: 2005
• 期刊: Electrophoresis 26
• 作者: Shuji Kodama;Atsushi Yamamoto;Reiko Iio;Sen-ichi Aizawa;Kazuya Nakagomi;Kazuichi Hayakawa
• 通讯作者: Kazuichi Hayakawa