Photoactive hemolytic toxin in Chattonella marina

查托氏菌码头中的光活性溶血毒素

基本信息

  • 批准号:
    16580154
  • 负责人:
  • 金额:
    $ 2.24万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    2004
  • 资助国家:
    日本
  • 起止时间:
    2004 至 2005
  • 项目状态:
    已结题

项目摘要

Chattonella marina (C.marina), a raphidophycean flagellate, is a causative organism of red tide, and highly toxic to fish. In this study, we found that the cell-free methanol extract prepared from this flagellate exhibited potent hemolytic activity against rabbit erythrocytes. Interestingly, the hemolytic activity of the extract was absolutely light-dependent, and no hemolytic activity was detected in the dark even at very high concentration. Gel filtration chromatography of the methanol extract on a column of Sephadex LH-20 revealed that the extract contained hemagglutinin as well as hemolytic agents, and the substances responsible for these activities were separately eluted. These results suggest that the hemagglutinating and hemolytic activities were derived from distinct compounds. The hemolytic fraction obtained after gel filtration (F4) caused marked inhibition of the growth of C.marina itself and other species of phytoplanktons. Furthermore, F4 showed a potent cytotoxicity toward various mammalian cultured cell lines including human tumor cells (HeLa cells) in a dose-dependent manner. The cytotoxicity was also light-dependent, and no cytotoxic effect was exhibited in any cell lines tested in the dark. After further purification procedures via preparative thin-layer chromatography and subsequent HPLC, a major hemolytic agent was obtained as highly purified form. Since the methanol extracts prepared from other raphidophycean flagellates such as Heterosigma akashiwo, Olisthodiscus luteus, and Fibrocapsa japonica showed light-dependent hemolytic activity toward rabbit erythrocytes, it was suggested that the light-dependent hemolytic agents commonly exist at least in these raphidophycean flagellates.
滨查通菌(Chattonella marina, C.marina)是一种鞭毛虫,是赤潮的致病生物,对鱼类有很高的毒性。在这项研究中,我们发现从这种鞭毛中提取的无细胞甲醇提取物对兔红细胞具有强效的溶血活性。有趣的是,提取物的溶血活性完全依赖于光,即使在非常高的浓度下,在黑暗中也没有检测到溶血活性。在Sephadex LH-20色谱柱上对甲醇提取物进行凝胶过滤层析,发现提取物中含有血凝素和溶血剂,并分别洗脱了这些活性物质。这些结果表明,血凝和溶血活性来源于不同的化合物。凝胶过滤后的溶血部分(F4)对c.m ina本身和其他种类的浮游植物的生长有明显的抑制作用。此外,F4对包括人肿瘤细胞(HeLa细胞)在内的多种哺乳动物培养细胞系显示出强烈的细胞毒性,且呈剂量依赖性。细胞毒性也是光依赖性的,在黑暗中测试的任何细胞系都没有表现出细胞毒性作用。通过制备薄层色谱和随后的高效液相色谱进一步纯化后,获得了一种高度纯化的主要溶血剂。由于赤石异角藻、黄斑鱼和日本纤维藻等鞭毛虫的甲醇提取物对家兔红细胞具有光依赖性溶血活性,提示至少在这些鞭毛虫中普遍存在光依赖性溶血剂。

项目成果

期刊论文数量(34)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Direct evidence for the reduction of water flow across the gills of Jack mackerel (Trachurys japonicus) by a harmful alga Chattonella marina.
有害藻类查托氏菌 (Chattonella marina) 减少流经竹荚鱼 (Trachurys japonicus) 鳃的水流的直接证据。
Characterization of the recombinant CEL-I, a GaINAc-specific C-type lection, expressed in Escherichia coli using an artificial synthetic gene.
使用人工合成基因在大肠杆菌中表达的重组 CEL-I(一种 GaINAc 特异性 C 型基因座)的表征。
  • DOI:
  • 发表时间:
    2004
  • 期刊:
  • 影响因子:
    0
  • 作者:
    T.Hatakeyama;K.Shiba;N.Matsuo;T.Fujimoto;T.Oda;H.Sugawara;H.Aoyagi
  • 通讯作者:
    H.Aoyagi
Purification and characterization of photosensitizing hemolytic toxin from harmful red tide phytoplankton, Heterocapsa circularisquama
  • DOI:
    10.1016/j.aquatox.2005.04.005
  • 发表时间:
    2005-07-30
  • 期刊:
  • 影响因子:
    4.5
  • 作者:
    Miyazaki, Y;Nakashima, T;Oda, T
  • 通讯作者:
    Oda, T
Characterization of the recombinant CEL-I,a GalNAc-specific C-type lectin,expressed in Escherichia coli using an arfificial synthetic gene.
使用人工合成基因在大肠杆菌中表达的重组 CEL-I(一种 GalNAc 特异性 C 型凝集素)的表征。
  • DOI:
  • 发表时间:
    2004
  • 期刊:
  • 影响因子:
    0
  • 作者:
    T.Hatakeyama;K.Shiba;N.Matsuo;T.Fujimoto;T.Oda;H.Sugawara;H.Aoyagi
  • 通讯作者:
    H.Aoyagi
Characterization of the recombinant CEL-I, a GalNAc-specific C-type lectin, expressed in Escherichia coli using an artificial synthetic gene.
使用人工合成基因在大肠杆菌中表达的重组 CEL-I(一种 GalNAc 特异性 C 型凝集素)的表征。
  • DOI:
  • 发表时间:
    2004
  • 期刊:
  • 影响因子:
    0
  • 作者:
    T.Hatakeyama;K.Shiba;N.Matsuo;T.Fujimoto;T.Oda;H.Sugawara;H.Aoyagi
  • 通讯作者:
    H.Aoyagi
{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

ODA Tatsuya其他文献

ODA Tatsuya的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('ODA Tatsuya', 18)}}的其他基金

Novel cancer therapy strategies, targeting cancer cell surface glycans using lectins (=glycan binding protein) as drug carriers.
新的癌症治疗策略,使用凝集素(=聚糖结合蛋白)作为药物载体靶向癌细胞表面聚糖。
  • 批准号:
    18H04057
  • 财政年份:
    2018
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Glycan targeting cancer therapy; targeting cancer cell surface glycan by using a lectin as a drug carrier.
聚糖靶向癌症治疗;
  • 批准号:
    16K15605
  • 财政年份:
    2016
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Possible toxic factors in the glycocalyx of Chattonella
查托氏菌糖萼中可能存在的毒性因素
  • 批准号:
    15K07580
  • 财政年份:
    2015
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Anthropological Research about Visiting to the Location Scenes in Digital Contents.
关于访问数字内容中的外景场景的人类学研究。
  • 批准号:
    24520931
  • 财政年份:
    2012
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Effective delivery of chemotherapeutic nanoparticles by depletinghost Kupffer cells
通过消耗宿主库普弗细胞有效递送化疗纳米颗粒
  • 批准号:
    23659635
  • 财政年份:
    2011
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Realizing the induction heating cancer therapy by developing new iron nano particle and combination of tumor penetrating peptide (iRGD).
通过开发新型铁纳米粒子与肿瘤穿透肽(iRGD)组合,实现感应加热癌症治疗。
  • 批准号:
    23300362
  • 财政年份:
    2011
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Microbioassay for the analysis of toxic factors produced by red tide phytoplankton
赤潮浮游植物有毒因子分析的微生物测定法
  • 批准号:
    23580255
  • 财政年份:
    2011
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Cancer specific delivery of magnetic nanoparticles.
磁性纳米颗粒的癌症特异性递送。
  • 批准号:
    21591743
  • 财政年份:
    2009
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Comparative study on the toxic factors of Chochlodinium polykrikoides and Chattonella marina
多环藻与海藻的毒性因素比较研究
  • 批准号:
    20580204
  • 财政年份:
    2008
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Ligand-toxin producing cell containing micro-capsule cancer therapy
含有微胶囊的配体毒素产生细胞癌症治疗
  • 批准号:
    18390359
  • 财政年份:
    2006
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)

相似海外基金

Genotypic diversity and phylogeography of harmful raphidophyte Chattonella in Southeast Asia
东南亚有害针叶植物 Chattonella 的基因型多样性和系统发育地理学
  • 批准号:
    19H03027
  • 财政年份:
    2019
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Functional analysis of aureochrome in red tide alga, Chattonella
赤潮藻Chatonella中金色素的功能分析
  • 批准号:
    17K07891
  • 财政年份:
    2017
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Studies on the mechanism for bloom formation of the red tide causing flagellates Chattonella (Raphidophyceae)
鞭毛虫Chattonella(剑藻纲)赤潮水华形成机制研究
  • 批准号:
    16K07823
  • 财政年份:
    2016
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Possible toxic factors in the glycocalyx of Chattonella
查托氏菌糖萼中可能存在的毒性因素
  • 批准号:
    15K07580
  • 财政年份:
    2015
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
A study on search of protein species which contribute to growth of a harmful alga Chattonella
寻找有助于有害藻类查托氏菌生长的蛋白质种类的研究
  • 批准号:
    23780197
  • 财政年份:
    2011
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
Studies on the marine microbial communities associated with the bloom of Chattonella anitiqua/marina
与 Chattonella anitiqua/marina 水华相关的海洋微生物群落研究
  • 批准号:
    22580202
  • 财政年份:
    2010
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Comparative study on the toxic factors of Chochlodinium polykrikoides and Chattonella marina
多环藻与海藻的毒性因素比较研究
  • 批准号:
    20580204
  • 财政年份:
    2008
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Study of Chattonella life cycle by using microsatellite markers
利用微卫星标记研究查托氏菌的生命周期
  • 批准号:
    19580225
  • 财政年份:
    2007
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Nitric oxide synthase(NOS) like enzyme mediated nitric oxide(NO) generation by harmful red tide phytoplankton, Chattonella marina
一氧化氮合酶(NOS)样酶介导有害赤潮浮游植物、查托氏菌码头产生一氧化氮(NO)
  • 批准号:
    18580205
  • 财政年份:
    2006
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Biochemical analysis of the enzymatic complex responsible for the generation of superoxide anion on the cell surface of Chattonella marina
对查托氏菌码头细胞表面产生超氧阴离子的酶复合物进行生化分析
  • 批准号:
    14560154
  • 财政年份:
    2002
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了