Comparative study on the toxic factors of Chochlodinium polykrikoides and Chattonella marina

多环藻与海藻的毒性因素比较研究

• 批准号: 20580204
• 负责人: ODA Tatsuya
• 金额: $ 3万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20580204/
• 关键词: プランクトン; 赤潮; 毒性; 活性酸素; 鞭毛藻類; 粘液

Generation of reactive oxygen species (ROS) by Chattonella marina could be confirmed by ESR spin trapping method. Immunohistochemical analysis of gill lamellae from yellowtail exposed to C.marina using antiserum against crude glycocalyx demonstrated that there were the antigns recognaised by the antiserum on the surface of gill lamellae. As compared to C.marina, the levels of ROS detected in Cochlodinium polykrikoides were trace levels. Furthermore, no significant increase in O^2_- generation by C.polykrikoides was observed in the presence of lectins or fish mucus prepared from skin and gill of yellowtail, whereas C.marina generated increased level of O^2_- responding to these stimuli. The cell-free aqueous extract prepared from C.polykrikoides showed toxic effect on HeLa cells, but the extract of C.marina had almost no effect. Furthermore, gradual accumulation of polysaccharides in the medium was observed during the culture of C.polykrikoides, but no such change was observed in the medium of C.marina. These results suggest that ROS may play a significant role in the fish-killing activity of C.marina, whereas in the case of C.polykrikoides, certain toxic substances or polysaccharides are mainly responsible for the toxicity rather than ROS.

利用ESR自旋捕集技术证实了海洋查顿氏藻产生活性氧。用抗粗糖萼抗血清对暴露于海水中的黄尾鱼鳃片进行免疫组化分析，结果表明，鳃片表面有抗血清标记的抗原。与海洋梭藻相比，在多克里藻中检测到的ROS水平为痕量水平。此外，在存在凝集素或从黄尾鱼的皮肤和鳃制备的鱼粘液的情况下，没有观察到多克里柯拟栉孔扇贝产生O^2_-的显著增加，而海洋栉孔扇贝对这些刺激产生了响应的O^2_-水平增加。结果表明，多棱毛藻无细胞水提物对HeLa细胞有一定的毒性作用，而海水毛藻水提物对HeLa细胞几乎无毒性作用。此外，逐渐积累的多糖在培养基中观察到的C.polykrikoides的培养过程中，但没有观察到这样的变化在C. marina。这些结果表明，活性氧可能在玛丽娜C.marina的杀鱼活性中发挥重要作用，而对于多克里藻C.polykrikoides，某些有毒物质或多糖而不是活性氧是导致毒性的主要原因。

项目成果

Cytotoxic action mode of a novel porphyrin derivative isolated from harmful red tide dinoflagellate Heterocapsa circularisquama.

从有害赤潮甲藻 Heterocapsa circularisquama 中分离出的新型卟啉衍生物的细胞毒性作用模式。

• 发表时间: 2008
• 期刊: J.Biochem.Mol.Toxicol. 22
• 作者: D.Kim;Y.Miyazaki;T.Nakashima;T.Iwashita;T.Fujita;K.Yamaguchi;K.-S.Choi;T.Oda
• 通讯作者: T.Oda

The role of interactions between Prorocentrum minimum and Heterosigma akashiwo in bloom formation

• DOI: 10.1007/s10750-009-0052-y
• 发表时间: 2010-01
• 期刊: Hydrobiologia
• 影响因子: 2.6
• 作者: Y. Yamasaki;S. Nagasoe;M. Tameishi;T. Shikata;Y. Zou;Z. Jiang;T. Matsubara;Y. Shimasaki;K. Yama

未利用天然資源として有望な赤潮プランクトン

赤潮浮游生物是一种有前途的未开发自然资源

• 发表时间: 2009
• 期刊: 養殖 4
• 作者: Yagi Y;I Kinoshita;S Fujita;H Ueda;D Aoyama.;伊藤毅史・古満啓介・山口敦子;鈴木秀和;伊東優太;山口敦子・古満啓介・久米元;小田達也
• 通讯作者: 小田達也

Comparison of the responses of peritoneal macrophages from Japanese flounder (Paralichthys olivaceus) against high-irulent and low-virulent strains of Edwardsiella tarda.

日本牙鲆（Paralichthys olivaceus）腹膜巨噬细胞对高毒力和低毒力爱德华氏菌菌株的反应比较。

• 发表时间: 2008
• 期刊: Fish and Shellfish Immunology 24
• 作者: K.Ishibe;K.Osatom;K.Hara;K.Kanai;K.Yamaguchi;T.Oda
• 通讯作者: T.Oda

Molecular mechanism of ROS (reactive oxygen species) generation by unicellular marine phytoplankton Chattonella marina.

单细胞海洋浮游植物查托氏菌码头产生ROS（活性氧）的分子机制。

• 发表时间: 2009
• 作者: D.Kim;T.Oda.
• 通讯作者: T.Oda.