Comparative study on the toxic factors of Chochlodinium polykrikoides and Chattonella marina

多环藻与海藻的毒性因素比较研究

• 批准号: 20580204
• 负责人: ODA Tatsuya
• 金额: $ 3万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20580204/
• 关键词: プランクトン; 赤潮; 毒性; 活性酸素; 鞭毛藻類; 粘液

Generation of reactive oxygen species (ROS) by Chattonella marina could be confirmed by ESR spin trapping method. Immunohistochemical analysis of gill lamellae from yellowtail exposed to C.marina using antiserum against crude glycocalyx demonstrated that there were the antigns recognaised by the antiserum on the surface of gill lamellae. As compared to C.marina, the levels of ROS detected in Cochlodinium polykrikoides were trace levels. Furthermore, no significant increase in O^2_- generation by C.polykrikoides was observed in the presence of lectins or fish mucus prepared from skin and gill of yellowtail, whereas C.marina generated increased level of O^2_- responding to these stimuli. The cell-free aqueous extract prepared from C.polykrikoides showed toxic effect on HeLa cells, but the extract of C.marina had almost no effect. Furthermore, gradual accumulation of polysaccharides in the medium was observed during the culture of C.polykrikoides, but no such change was observed in the medium of C.marina. These results suggest that ROS may play a significant role in the fish-killing activity of C.marina, whereas in the case of C.polykrikoides, certain toxic substances or polysaccharides are mainly responsible for the toxicity rather than ROS.

用ESR自旋捕捉法证实了海洋查顿氏菌产生的ROS。用粗糖萼抗血清对暴露于滨海梭子蟹的黄尾鱼鳃片进行免疫组织化学分析，结果表明，鳃片表面有抗血清识别的抗血清。与滨海梭子藻相比，其体内的ROS含量为微量水平。此外，在黄尾鱼皮肤和鳃制备的凝集素或鱼粘液的存在下，波氏梭子藻产生的O^2-没有显著增加，而滨海梭子蟹对这些刺激产生的O^2-反应水平增加。白花蛇舌草的无细胞水提物对HeLa细胞有明显的毒性作用，而滨海金鱼提取液对HeLa细胞几乎没有影响。此外，在培养过程中观察到多糖在培养基中的逐渐积累，而在滨海梭子藻的培养中则没有观察到这种变化。这些结果表明，ROS可能在滨海梭子蟹的杀鱼活性中起着重要的作用，而对于波氏梭子藻来说，某些有毒物质或多糖是主要的毒性物质或多糖，而不是ROS。

项目成果

Cytotoxic action mode of a novel porphyrin derivative isolated from harmful red tide dinoflagellate Heterocapsa circularisquama.

从有害赤潮甲藻 Heterocapsa circularisquama 中分离出的新型卟啉衍生物的细胞毒性作用模式。

• 发表时间: 2008
• 期刊: J.Biochem.Mol.Toxicol. 22
• 作者: D.Kim;Y.Miyazaki;T.Nakashima;T.Iwashita;T.Fujita;K.Yamaguchi;K.-S.Choi;T.Oda
• 通讯作者: T.Oda

The role of interactions between Prorocentrum minimum and Heterosigma akashiwo in bloom formation

• DOI: 10.1007/s10750-009-0052-y
• 发表时间: 2010-01
• 期刊: Hydrobiologia
• 影响因子: 2.6
• 作者: Y. Yamasaki;S. Nagasoe;M. Tameishi;T. Shikata;Y. Zou;Z. Jiang;T. Matsubara;Y. Shimasaki;K. Yama

未利用天然資源として有望な赤潮プランクトン

赤潮浮游生物是一种有前途的未开发自然资源

• 发表时间: 2009
• 期刊: 養殖 4
• 作者: Yagi Y;I Kinoshita;S Fujita;H Ueda;D Aoyama.;伊藤毅史・古満啓介・山口敦子;鈴木秀和;伊東優太;山口敦子・古満啓介・久米元;小田達也
• 通讯作者: 小田達也

Comparison of the responses of peritoneal macrophages from Japanese flounder (Paralichthys olivaceus) against high-irulent and low-virulent strains of Edwardsiella tarda.

日本牙鲆（Paralichthys olivaceus）腹膜巨噬细胞对高毒力和低毒力爱德华氏菌菌株的反应比较。

• 发表时间: 2008
• 期刊: Fish and Shellfish Immunology 24
• 作者: K.Ishibe;K.Osatom;K.Hara;K.Kanai;K.Yamaguchi;T.Oda
• 通讯作者: T.Oda

Molecular mechanism of ROS (reactive oxygen species) generation by unicellular marine phytoplankton Chattonella marina.

单细胞海洋浮游植物查托氏菌码头产生ROS（活性氧）的分子机制。

• 发表时间: 2009
• 作者: D.Kim;T.Oda.
• 通讯作者: T.Oda.