Microbioassay for the analysis of toxic factors produced by red tide phytoplankton

赤潮浮游植物有毒因子分析的微生物测定法

• 批准号: 23580255
• 负责人: ODA Tatsuya
• 金额: $ 3.41万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23580255/
• 关键词: プランクトン; 赤潮; 毒性; 培養細胞; バイオアッセイ; 細胞培養

Harmful algal blooms (HABs) are one of serious environmental problems. To investigate the toxic mechanism of HABs species, direct exposure experiments against fish or shellfish have been conducted. However, such exposure experiments generally require special animal rearing facilities and mass-culture of phytoplankton. Usage of appropriate cultured cells as an alternative for whole living animals seems to be a promising strategy. We evaluated lactate dehydrogenase (LDH)-release assay as a simple estimation method for toxic potential of harmful algal species at cellular level. Live cell suspension of Karenia mikimotoi (strain SUO-1), which is highly toxic to rotifer and has potent hemolytic activity, induced the release of significant levels of LDH from all the cell lines tested in cell-density and time-dependent manners, whereas another strain of K. mikimotoi (strain FUK), which is less-toxic to rotifer and has low hemolytic activity, showed fairly low activities.

有害藻华（HABs）是严重的环境问题之一。为了研究有害藻华的毒性机制，对鱼类或贝类进行了直接暴露实验。然而，这种暴露实验通常需要特殊的动物饲养设施和浮游植物的大量培养。使用适当的培养细胞作为整个活体动物的替代品似乎是一个有前途的策略。我们评价乳酸脱氢酶（LDH）释放法在细胞水平上作为一种简单的估计有害藻类毒性潜能的方法。mikimotoi Karenia （SUO-1）活细胞悬液对轮虫具有高毒性和强溶血活性，在细胞密度和时间依赖性方面均能诱导所有细胞株释放显著水平的LDH，而另一株对轮虫毒性较小且溶血活性较低的mikimotoi Karenia （FUK）活细胞悬液则表现出相当低的活性。

项目成果

Evaluation of LDH-release Assay for the Detection and Analysis of Cellular Level Toxic Potential of HAB species

用于检测和分析 HAB 物种细胞水平毒性潜力的 LDH 释放测定法的评估

• 发表时间: 2012
• 作者: Yanan Zou;Daekyung Kim;Yasuhiro Yamasaki;Kenichi Yamaguchi;Tatsuya Oda
• 通讯作者: Tatsuya Oda

Evaluation of toxic potential of newly isolated Chattonella antiqua, by laboratory exposure experiments and micro-bioassay using cultured cells

通过实验室暴露实验和使用培养细胞的微生物测定来评估新分离的古查托氏菌的毒性潜力

• 发表时间: 2012
• 作者: Junya Tomita;Tomoki Nishiguchi;Motoaki Yagi;Daekyung Kim;and Tatsuya Oda
• 通讯作者: and Tatsuya Oda

Macrophage-stimulating activity of sulfated polysaccharide ascophyllan isolated from marine brown alga Ascophyllum nodosum.

从海洋褐藻泡叶藻中分离的硫酸化多糖泡叶聚糖的巨噬细胞刺激活性。

• 发表时间: 2013
• 作者: Zedong Jiang;Takasi Okimura;Kenichi Yamaguchi;Tatsuya Oda.
• 通讯作者: Tatsuya Oda.

Detection and characterization of bio-toxic factors produced by red tide phytoplankton Heterocapsa circularisquama by micro-plate bioassay.

微孔板生物测定法检测和表征赤潮浮游植物 Heterocapsacircularisquama 产生的生物毒性因子。

• 发表时间: 2011
• 作者: Motomura;H.;Y. Takigawa;G. Ogihara and T. Yoshino;Daekyung Kim and Tatsuya Oda
• 通讯作者: Daekyung Kim and Tatsuya Oda

渦鞭毛藻 Karenia mikimotoiの細胞毒性に関する研究.

甲藻Karenia mikimotoi的细胞毒性研究。

• 发表时间: 2011
• 作者: 鄒亜男;山口健一;小田達也
• 通讯作者: 小田達也