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Lectin-induced enhancement of superoxide anion production by red tide plankton, Chattonella marina

凝集素诱导赤潮浮游生物、查托氏菌码头超氧阴离子产生的增强

基本信息

批准号：
10660186
负责人：
ODA Tatsuya
金额：
$ 2.24万
依托单位：
Nagasaki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10660186/
关键词：
Red Tide Plankton Reactive Oxygen Species Toxicity Chattonella Raphyidophyceae Lectin

项目摘要

Chattonella marina, a raphidophycean flagellate, is one of the most noxious red tide phytoplankton and is highly toxic to fish, especially to yellowtail Seriola quinqueradiata. Although the precise mechanism of the toxic action of Chattonella sp. remains unclear, it has been demonstrated that a decrease in oxygen partial pressure of arterial blood is the earliest physiological disturbance observed in fish after exposure to C. marina, Physiological and histological studies of fish exposed to C. marina also suggested that the blockade of respiratory water flow through the gill lamellae caused by excessive mucus interferes with O2 transfer, resulting in asphyxia. Recent studies have shown that Chattonella spp. Generate reactive oxygen species (ROS) such as O2-, H2O2, and hydroxyl radical (・OH). Furthermore, it has been reported that Heterosigma akashiwo, which is also classified in a raphidophycean, produces ROS, and showed an oxygen-radical-mediated toxic effect on rainbow trout, Oncorhy … More nchus mykiss. Thus, it is likely that the production of ROS is a common feature of raphidophycean flagellates. In the present study, we found that lectins such as concanavalin A (Con A), wheat germ agglutinin (WGA), and castor bean hemagglutinin (CBH) stimulated C. marina and H. akashiwo to generate enhanced amounts of O2- in a concentration dependent manner. The lectin-specific sugars potently inhibited the lectin-induced increase of O2-production, suggesting that the effects of lectins are mediated mainly through the interaction of these lectins with carbohydrate moiety present on the flagellate cell surface. In contrast to the potent ability of native Con A (tetravalent), succinylated Con A (divalent) showed only slight stimulative effect on these flagellates. O2- production was totally inhibited by treatment with proteinase K for 30 min, without affecting the viabilities of flagellates. These results suggest that cell-surface redox enzyme may be involved in O2- production, and such enzyme is responsible for the lectin-stimulation. Less

海洋轮藻是一种鞭毛藻类，是最具毒性的赤潮浮游植物之一，对鱼类，特别是对五叶黄尾轮虫有很强的毒性。尽管Chattonella sp.毒力作用的确切机制尚不清楚。尽管目前尚不清楚，但已有研究表明，暴露于滨海弧菌后，最早观察到的生理障碍是动脉血氧分压的降低。暴露于滨海弧菌的鱼类的生理和组织学研究也表明，过量的粘液阻碍了呼吸水流通过颧板的流动，干扰了O2的传递，导致窒息。最近的研究表明，查顿氏菌(Chattonella spp.)产生活性氧物种(ROS)，如O2-、H2O2和羟基自由基(·OH)。此外，赤潮异弯藻(Heterosigma Akashiwo)也被归类为藻类，它能产生ROS，并对虹鱼Oncorhy…产生氧自由基介导的毒性作用。更多的是我的吻。因此，ROS的产生很可能是石藻鞭毛虫的共同特征。在本研究中，我们发现刀豆蛋白A(ConA)、小麦胚芽凝集素(WGA)和蓖麻豆血凝素(CBH)等凝集素以浓度依赖的方式刺激滨海假单胞菌和赤潮假单胞菌产生大量的O2-。凝集素特异性糖能有效地抑制凝集素诱导的O2-生成增加，这表明凝集素的作用主要是通过这些凝集素与鞭毛细胞表面碳水化合物部分的相互作用来实现的。与天然ConA(四价)的强大能力相比，琥珀酸化的ConA(二价)对这些鞭毛只有轻微的刺激作用。用K酶处理30min，可完全抑制O2-产生，但不影响鞭毛虫的存活率。这些结果表明，细胞表面氧化还原酶可能参与O2-的产生，这种酶负责凝集素的刺激。较少