Effect of Kosa on Th1 and Th2 immune responses and on oral tolerance

研究了番薯对小鼠Th1和Th2免疫应答及口服耐受性的影响。Th1反应似乎参与了一些自身免疫性疾病，如类风湿关节炎，Th2参与了过敏性疾病，包括花粉诱导的鼻炎。口服耐受似乎在预防食物过敏中起着重要作用。为了诱导Th1和Th2免疫应答，用卵清蛋白（OVA）免疫DBA小鼠（第0天）。第21天，检测抗ova IgG2a抗体和IFN-g作为Th1免疫应答指标，检测抗ova IgG1抗体和IgE抗体以及IL-4作为Th2免疫应答指标。TGF-b和IL-10分别作为th3和调节性T细胞介导的免疫应答指标。从第5天到第1天，分别饲喂20和0.1 mg OVA诱导小鼠对Th1和Th2的口服耐受。从第0天开始，在21天内每天口服不同剂量的科沙，以测试其对Th1和Th2反应的影响。为了研究科沙对口服耐受的影响，从第5天到第1天每天口服科沙。PBS作为对照。结果表明，Kosa对抗ova IgG2a和IFN-g的产生没有影响。相比之下，Kosa促进了抗ova IgE和IL-4的分泌，但抗ova IgGl的产生不受Kosa的影响。在Kosa和pbs处理的小鼠之间，TGF-b和IL-10的产生没有差异。Kosa似乎增强了Th2而非Th1的口服耐受性。这些结果表明，Kosa可能促进Th2而不是Th1的免疫反应。Kosa也可增强Th2而非Th1的口服耐受性。Th3和调节性T细胞的功能似乎不受Kosa的影响。

The effects of Kosa on Th1 and Th2 immune responses and on oral tolerance in mice were investigated. Th1 responses appear to be involved in some of autoimmune diseases such as rheumatoid arthritis and Th2 in allergic diseases including pollen-induced rhinitis. Oral tolerance appears to play an important role in preventing food allergy. To induce Th1 and Th2 immune responses, DBA mice were immunized with ovalbumin (OVA) (day 0). On day 21, anti-OVA IgG2a antibodies and IFN-g were measured as indicators of Th1 immune responses and anti-OVA IgG1 and IgE antibodies and IL-4 as those of Th2 immune responses. TGF-b and IL-10 were also measured as indicators Th3-and regulatory T cell-mediated immune responses, respectively. Th1 and Th2 oral tolerance were induced by feeding mice 20 and 0.1 mg of OVA, respectively, from days-5 to-1. Varying doses of Kosa were orally administered daily over a period of 21 days commencing on day 0 to test its effects on Th1 and Th2 responses. To examine the effect of Kosa on oral tolerance, Kosa were orally given daily from days-5 to -1. PBS was fed as a control. The results showed that Kosa failed to affect production of anti-OVA IgG2a and IFN-g. In contrast, anti-OVA IgE and IL-4 secretion was facilitated by Kosa, although anti-OVA IgGl production was unaffected by Kosa. There was no difference in production of TGF-b and IL-10 between Kosa and PBS-treated mice. Th2 but not Th1 oral tolerance appeared to be enhanced by Kosa. These results suggest that Kosa may facilitate Th2 but not Th1 immune responses. Th2 but not Th1 oral tolerance also may be enhanced by Kosa. The function of Th3 and regulatory T cells does not appear to be affected by Kosa.