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Dynamic analysis of tight junction strands

Dynamic analysis of tight junction strands
紧密连接链的动态分析
批准号:
16590155
负责人:
SASAKI Hiroyuki
金额:
$1.6万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2004
资助国家:
日本
项目状态:
已结题
起止时间:
2004 至 2005

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中文摘要
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英文摘要
The tight junction (TJ) strand is a linear polymer, typical of proteinaceous polymers within plasma membranes, and the TJ strand itself is thought to be involved directly in the barrier and fence functions of TJs. I constructed the plasmid for expression of a fusion protein of the full length of claudin-1,the green fluorescent protein (GFP) and cytoplasmic COOH domain of claudin-1,and transfected the plasmid into Tet-Off cells derived from L type fibroblast cells and Madin-Darby Canine Kidney I cells. Then, I attempted to visualize individual TJ strands in the fibroblast or epithelial cells. These studies would reveal to what extent the behavior of the in situ TJ strands is dynamic as compared to the reconstituted paired claudin strands, and how the dynamic characteristics of the TJ strands are modified and/or regulated in epithelial/endothelial cells.
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DOI: 10.1083/jcb.200501154
发表时间: 2005-05-09
期刊: The Journal of cell biology
影响因子: --
作者: [Miyamoto T, Morita K, Takemoto D, Takeuchi K, Kitano Y, Miyakawa T, Nakayama K, Okamura Y, Sasaki H, Miyachi Y, Furuse M, Tsukita S]
通讯作者: Tsukita S
Electron tomography of fast frozen, stretched rigor ibers reveals elastic distortions in the myosin crossbridges.
快速冷冻、拉伸的刚性纤维的电子断层扫描揭示了肌球蛋白横桥的弹性变形。
DOI: --
发表时间: 2004
期刊: J Struct Biol 147
影响因子: --
作者: [J.Liu, M.C.Reedy, Y.Goldman, C.Armstrong, H.Sasaki, et al.]
通讯作者: et al.
DOI: 10.1074/jbc.m402616200
发表时间: 2004-10-29
期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
影响因子: 4.8
作者: [Ohnishi, H, Nakahara, T, Furuse, M]
通讯作者: Furuse, M
Radixin deficiency causes deafness associated with progressive degeneration of cochlear stereocilia.
根黄素缺乏会导致与耳蜗静纤毛进行性退化相关的耳聋。
DOI: 10.1083/jcb.200402007
发表时间: 2004-08-16
期刊: The Journal of cell biology
影响因子: --
作者: [Kitajiri S, Fukumoto K, Hata M, Sasaki H, Katsuno T, Nakagawa T, Ito J, Tsukita S, Tsukita S]
通讯作者: Tsukita S
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