Dynamic analysis of tight junction strands

紧密连接链的动态分析

• 批准号: 16590155
• 负责人: SASAKI Hiroyuki
• 金额: $ 1.6万
• 依托单位: The Jikei University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16590155/
• 关键词: Tight junction; Claudin; GFP; Tet-Off; ZO-1

The tight junction (TJ) strand is a linear polymer, typical of proteinaceous polymers within plasma membranes, and the TJ strand itself is thought to be involved directly in the barrier and fence functions of TJs. I constructed the plasmid for expression of a fusion protein of the full length of claudin-1,the green fluorescent protein (GFP) and cytoplasmic COOH domain of claudin-1,and transfected the plasmid into Tet-Off cells derived from L type fibroblast cells and Madin-Darby Canine Kidney I cells. Then, I attempted to visualize individual TJ strands in the fibroblast or epithelial cells. These studies would reveal to what extent the behavior of the in situ TJ strands is dynamic as compared to the reconstituted paired claudin strands, and how the dynamic characteristics of the TJ strands are modified and/or regulated in epithelial/endothelial cells.

紧密连接（TJ）链是一种线性聚合物，是质膜内蛋白质聚合物的典型代表，TJ链本身被认为直接参与TJ的屏障和栅栏功能。我构建了用于表达claudin-1全长、claudin-1的绿色荧光蛋白（GFP）和胞质COOH结构域的融合蛋白的质粒，并将该质粒转染到源自L型成纤维细胞和Madin-Darby犬肾I细胞的Tet-Off细胞中。然后，我试图可视化成纤维细胞或上皮细胞中的单个TJ链。这些研究将揭示原位TJ链的行为与重构的成对紧密连接蛋白链相比在何种程度上是动态的，以及TJ链的动态特征如何在上皮/内皮细胞中被修饰和/或调节。

项目成果

Tight junctions in Schwann cells of peripheral myelinated axons: a lesson from claudin-19-deficient mice.

• DOI: 10.1083/jcb.200501154
• 发表时间: 2005-05-09
• 期刊: The Journal of cell biology
• 作者: Miyamoto T;Morita K;Takemoto D;Takeuchi K;Kitano Y;Miyakawa T;Nakayama K;Okamura Y;Sasaki H;Miyachi Y;Furuse M;Tsukita S
• 通讯作者: Tsukita S

Electron tomography of fast frozen, stretched rigor ibers reveals elastic distortions in the myosin crossbridges.

快速冷冻、拉伸的刚性纤维的电子断层扫描揭示了肌球蛋白横桥的弹性变形。

• 发表时间: 2004
• 期刊: J Struct Biol 147
• 作者: J.Liu;M.C.Reedy;Y.Goldman;C.Armstrong;H.Sasaki;et al.
• 通讯作者: et al.

JACOP, a novel plaque protein localizing at the apical junctional complex with sequence similarity to cingulin

• DOI: 10.1074/jbc.m402616200
• 发表时间: 2004-10-29
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Ohnishi, H;Nakahara, T;Furuse, M
• 通讯作者: Furuse, M

Radixin deficiency causes deafness associated with progressive degeneration of cochlear stereocilia.

根黄素缺乏会导致与耳蜗静纤毛进行性退化相关的耳聋。

• DOI: 10.1083/jcb.200402007
• 发表时间: 2004-08-16
• 期刊: The Journal of cell biology
• 作者: Kitajiri S;Fukumoto K;Hata M;Sasaki H;Katsuno T;Nakagawa T;Ito J;Tsukita S;Tsukita S
• 通讯作者: Tsukita S

Specificity and biomineralization activities of Ti-binding peptide-1 (TBP-1)

• DOI: 10.1021/la047428m
• 发表时间: 2005-03-29
• 期刊: LANGMUIR
• 影响因子: 3.9
• 作者: Sano, KI;Sasaki, H;Shiba, K
• 通讯作者: Shiba, K