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Investigation on the immune reactions against complex antigens including gangliosides in the neuroimmunological diseases

神经免疫疾病中神经节苷脂等复杂抗原的免疫反应研究

基本信息

批准号：
16590854
负责人：
KUSUNOKI Susumu
金额：
$ 2.24万
依托单位：
Kinki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

项目摘要

Antiganglioside antibodies are frequently present in sera from patients with neuroimmunological diseases as Guillain Barre syndrome (GBS) and Miller Fisher syndrome (MFS). Each ganglioside coexists with phospholipids and other gangliosides in the plasma membrane. We investigated antibody reactivities against complex antigens composed of a ganglioside and a phospholipid or those composed of two species of gangliosides. Anti-GM1 IgG antibodies in GBS had stronger binding activities against a mixture of GM1 and such phospholipids as phosphatidic acid than against GM1 alone. However, such increase was not observed in anti-GQ1b IgG antibodies in MFS. This may be due to the difference in the amount of the negatively-charged sialic acid that each antigen has ; GM1 has one sialic acid whereas GQ1b has four sialic acids. Some GBS patients had the antibodies specific to the ganglioside complex, which is composed of two different gangliosides. Among them, the antibodies specific to GD1a-GD1b complex and/or GD1b-GT1b complex are associated with severe GBS requiring artificial ventilation. In addition, some MFS patients had the antibodies against ganglioside complexes that include GQ1b. For elucidating the pathogenetic roles of the antiganglioside antibodies in neuroimmunological diseases, we should pay attention on the antibody activities against those complex antigens. Investigating antibodies against the complex antigens also may be useful for diagnosis of neuroimmunological diseases as GBS and MFS.

抗神经节苷脂抗体经常存在于格林-巴利综合征（GBS）和米勒-费舍尔综合征（MFS）等神经免疫性疾病患者的血清中。每一种神经节苷脂与磷脂和其他神经节苷脂在质膜中共存。我们研究了抗体对由神经节苷脂和磷脂组成的复合抗原或由两种神经节苷脂组成的抗原的反应性。GBS抗GM1 IgG抗体对GM1与磷脂酸等磷脂混合物的结合活性比单独对GM1的结合活性强。然而，MFS中抗gq1b IgG抗体未观察到这种增加。这可能是由于每种抗原所含的带负电荷的唾液酸的数量不同；GM1有一个唾液酸，而GQ1b有四个唾液酸。一些GBS患者具有针对神经节苷脂复合物的抗体，该复合物由两种不同的神经节苷脂组成。其中，GD1a-GD1b复合物和/或GD1b-GT1b复合物特异性抗体与需要人工通气的严重GBS相关。此外，一些MFS患者具有抗神经节苷脂复合物的抗体，其中包括GQ1b。为了阐明抗神经节苷脂抗体在神经免疫性疾病中的发病作用，我们应该关注针对这些复杂抗原的抗体活性。研究针对复合抗原的抗体也可能对GBS和MFS等神经免疫学疾病的诊断有用。