Rapid quantification system of DNA copy number for the specific molecular targeting area in stomach cancer tissue using by quantitative real time PCR assay.

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In 2004, our group almost collected the information about the gene specific areas which were associated with the molecular targeting therapy, active immunotherapy and cancer chemotherapy. The quantification of ZNF217 DNA copy number which had been succeeded in previously was analyzed for more clinical samples. In 2005, using by quantitative real time microsatellite analysis (QuMA), the DNA copy number of AIB-1 in chromosome 20q and c-Myc in chromosome 8q were observed in stomach cancer. Further, our group focused on the MAGE-A family (MAGE-A10・MAGE-A3) as cancer testis antigens (CTA) and TRAG-3 related to the drug resistance of Taxan and the specific target for the active immunotherapy. Immunohistochemically, by using monoclonal antibody 6C1, MAGE-A family protein expression in cases with stomach cancer metastasized to the liver were significantly observed (about 70%) than those with early stage cancer and those with advanced stage cancer who were no recurrent for three years after surgery. In 2006, our group detected the MAGE -A10 mRNA signals by highly sensitive in situ hybridization (ISH) using cRNA probes since no specific monoclonal antibody for MAGE-A10 were not available. MAGE-A family expression by 6C1 and MAGE-A10 mRNA expression by highly sensitive ISH were well concordant. We think that IHC and highly sensitive ISH using cRNA probes were useful for the screening for the selection of the gene specific areas which could be measured DNA copy number by QuMA.