Molecular biological approach to therapy for acute lung injury using RNA interference

利用 RNA 干扰治疗急性肺损伤的分子生物学方法

• 批准号: 16591537
• 负责人: MIKAWA Katsuya
• 金额: $ 2.18万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16591537/
• 关键词: RNA interference; transfection; alveolar epithelium; macrophage; acute lung injury; cytokine; small interfering RNA; knockdown; RNC干渉

We planned some experiments using RNA interference in alveolar epithelial cells type II (AEC-II) to establish therapeutic strategy for acute lung injury. In preliminary study, we tried to seek the optimal method by which IL-8 small interfering RNA of human IL-8 is transfected to A549 (AEC-II-like human cell line) cells. To assess this, we obtained 21 base siRNA specific for mRNA sequence of human IL-8 labeled with rhodamine, and tried to introduce the siRNA to A549 cells using HVJ envelope vector. However, transfection efficiency was poor. Thus, we tried to take various measures to increase the efficiency, but failed to do so. Thus, we were unable to use the present system to the following experiments.

我们计划在肺泡上皮细胞II型（AEC-II）中使用RNA干扰来建立急性肺损伤的治疗策略。本研究初步探索了人IL-8的小干扰RNA（siRNA）转染A549细胞的最佳方法。为了评估这一点，我们获得了21个碱基的siRNA特异性的mRNA序列的人IL-8标记的罗丹明，并试图引入siRNA到A549细胞使用HVJ包膜载体。然而，转染效率差。因此，我们试图采取各种措施来提高效率，但没有成功。因此，我们无法使用本系统进行以下实验。