Effects of Growth Factors and Carcinogens on the Development of Odontogenic Tumors

生长因子和致癌物对牙源性肿瘤发生的影响

• 批准号: 16591849
• 负责人: KAMEYAMA Yoichiro
• 金额: $ 2.24万
• 依托单位: Aichi-Gakuin University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16591849/
• 关键词: animal; jaw bone; tooth germ; organ culture; heterocyclic amine; growth factor

In the present study, the effects of both growth factor TGF-α and carcinogen Trp-P-2 on the development of organ-cultured ddY mouse tooth-germ transplanted into the subcutaneous tissue of nude mice were investigated historically and immnohistochemically (PCNA) in the following 5 Groups of mice : Group A (Control), Group B (DMSO), Group C (TGF-α), Group D (Trp-P-2) and Group E (TGF-α+Trp-P-2). Historically, in Groups C,D and E, a marked proliferation of odontogenic epithelial cells was observed. In Groups D and E, keratinization of the odontogenic epithelial cells was found, but it was not observed in Group C. Moreover, in Group E, a cyst-like space was observed in the area of keratinized odontogenic epithelial cells. Immunohistochemically (PCNA), in Groups C,D and E, the positivities of the odontogenic epithelial cells were significantly higher than in Groups A and B. The results of the present study suggest that proliferation and keratinization of odontogenic epitherial cells of transplanted organ-cultured tooth-germs in mice are promoted by growth factor TGF-α and carcinogen Trp-P-2.

本研究采用历史和免疫组织化学（PCNA）方法研究了生长因子TGF-α和致癌物trp -2对移植到裸鼠皮下的器官培养的ddY小鼠牙胚发育的影响，并将其分为A组（对照组）、B组（DMSO）、C组（TGF-α）、D组（Trp-P-2）和E组（TGF-α+Trp-P-2）。历史上，C、D、E组牙源性上皮细胞明显增殖。D、E组牙源性上皮细胞出现角化，c组未见角化，E组牙源性上皮细胞角化区可见囊肿样间隙。免疫组化（PCNA）结果显示，C、D、E组的成牙性上皮细胞阳性明显高于A、b组。本研究结果提示，生长因子TGF-α和致癌物Trp-P-2促进移植器官培养牙胚小鼠成牙性上皮细胞的增殖和角化。

项目成果

Histological and Immunohistochemical Study on Effects of TGF-α and Trp-P-2 on the Transplanted Organ-Cultured Mouse Tooth-germ

TGF-α和Trp-P-2对移植器官培养小鼠牙胚影响的组织学和免疫组织化学研究

• 发表时间: 2006
• 期刊: Journal of Oral Medicine and Pathology 11
• 作者: Yoshihiko Sugita;Katsutoshi Kubo;Emiko Sato;Hatsuhiko Maeda;Yoichiro Kameyama
• 通讯作者: Yoichiro Kameyama

Histological and Immunohistochemical Study of Effects of TGF-α and Trp-P-2 on the Transplanted Organ-cultured Mouse Tooth-germ

TGF-α和Trp-P-2对移植器官培养小鼠牙胚影响的组织学和免疫组织化学研究

• 发表时间: 2006
• 期刊: Oral Medicine and Pathology (投稿中)
• 作者: Yoshihiko Sugita;Katsutoshi Kubo;Emiko Sato;Hatsuhiko Maeda;Yoichiro Kameyama
• 通讯作者: Yoichiro Kameyama