Effect of Carcinogenic Heterocyclic Amine, EGF, and FGF on the Tooth-germ

致癌杂环胺、EGF 和 FGF 对牙胚的影响

• 批准号: 11671831
• 负责人: KAMEYAMA Yoichiro
• 金额: $ 2.3万
• 依托单位: Aichi-Gakuin University School of Dentistry
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671831/
• 关键词: animal; oral mucosa; tooth germ; organ culture; heterocyclic amine; growth factor

I.In this study, the effects of applications of Trp-P-2 on the oral mucosa of the rat were investigated by histology and AgNOR silver staining and PCNA immunohistochemical staining methods. In Group A (control group), Gingiva and buccal mucosa showed a normal histological structure and also normal AgNOR counts and PCNA percentages. In Group B (DMSO group), Gingiva and buccal mucosa were similar in histology, AgNOR counts and PCNA percentages to those of Group A.In Group C (Trp-P-2 group), Gingiva and buccal mucosa displayed mild changes of epithelial displasia, which were not seen in those of Group A.These epithelial changes were irregular arrangement and cellular atypia of basal cells, irregular arrangement and proliferation of prickle cells, thickening of the keratinized cell layer and irregular elongation of epithelial projections. These changes were almost similar between gingiva and buccal mucosa. The AgNOR counts and PCNA percentages in gingiva and buccal mucosa were statisticall … More y significant (P<0.01) compared with those of Group A.The results of the present study suggested that application of Trp-P-2 produced the changes of epithelial dysplasia in gingiva and buccal mucosa of the animal.II.The purpose of the present study was to investigate by histology and BrdU method the effects of EGF (epidermal growth factor) and IGF-I (insulin-like growth factor-I) on the development of the mouse tooth-germ in organ culture. The tooth germs of Groups A (BGJb), B (EGF 100ng/ml), C (EGF 200ng/ml), D (IGF 50ng/ml), E (IGF 100ng/ml), F ( EGF 100ng/ml and IGF 50ng/ml) , G (EGF 100ng/ml and IGF 100ng/ml) , H (EGF 200ng/ml andIGF 50ng/ml ) and I ( EGF 200ng/ml and IGF 100ng/ml) were examined by histology and BrdU method. The epithelial cell proliferation of the tooth germs in Groups B and C were marked as compared with that in Group A.However, the epithelial cell proliferation of the tooth germs in Groups D and E were similar to that in Group A.Though the epithelial cell proliferation of the tooth germs in Groups F, G, H and I were marked as compared with that in Group A, it was similar to those in Groups B and C.The results of the present study indicated that EGF promoted the proliferation of epithelial cells in the mouse tooth germs in organ culture, but also suggested that IGF-I did not stimulate the proliferation of epithelial cells in the mouse tooth germs. Less

本研究通过组织学、AgNOR银染色和PCNA免疫组化染色方法，观察了Trp-P-2对大鼠口腔粘膜的影响。A组（对照组）牙龈和颊粘膜组织结构正常，AgNOR计数和PCNA百分比正常。B组DMSO组牙龈和颊粘膜组织学、AgNOR计数和PCNA百分比与A组相似（Trp-P-2组）牙龈和颊粘膜上皮细胞轻度增生，而A组未见，表现为基底细胞排列不规则、细胞增生，棘细胞排列不规则、细胞增生，角化细胞层增厚和上皮突起不规则伸长。这些变化在牙龈和颊粘膜之间几乎相似。牙龈和颊粘膜中AgNOR计数和PCNA百分比均显著高于对照组， ...更多信息 y显著结果提示：Trp-P-2可引起牙龈及颊粘膜上皮异常增生的改变。II.本研究旨在通过组织学和BrdU法观察EGF对牙龈及颊粘膜上皮异常增生的影响。表皮生长因子（epidermal growth factor，EGF）和胰岛素样生长因子-I（insulin-like growth factor-I，IGF-I）对小鼠牙胚发育的影响。A（BGJ B）、B组牙胚（EGF 100ng/ml），C（EGF 200ng/ml），D（IGF 50ng/ml）、E（IGF 100ng/ml），F（EGF 100ng/ml和IGF 50ng/ml）、G用组织学和BrdU法检测EGF（100 ng/ml）和IGF（100 ng/ml）、H（200 ng/ml和50 ng/ml）和I（200 ng/ml和100 ng/ml）。B、C组牙胚上皮细胞增殖较A组明显，D、E组牙胚上皮细胞增殖与A组相似，F、G、H、I组牙胚上皮细胞增殖较A组明显，本研究的结果表明，EGF促进了器官培养的小鼠牙胚上皮细胞的增殖，但也表明IGF-I没有刺激小鼠牙胚上皮细胞的增殖。少