Effects of TGF-alpha, Trp-P-2 and MeIQx on the Tooth-germ in Organ Culture

本研究的目的是通过组织学和免疫组织化学（BrdU）研究生长因子TGF- α和致癌杂环胺Trp-P-2和MelQx对器官培养小鼠牙胚的影响。将小鼠牙胚分为以下3个实验（1、2、3），共7组。实验一：I-A组（BGJb）和I-B组（TGF- α）。实验二世。II-A组（DMSO）， II-B组（Trp-P-2）和II-C组（MeIQx）。实验三。III-A组（TGF- α +Trp-P-2）和III-B组（TGF- α +MeIQx）。本研究结果表明TGF- α显著促进小鼠牙胚上皮细胞的增殖，但也提示TGF- α不刺激这些上皮细胞的分化。此外，还提示Trp-P-2和MeIQx抑制了这些上皮细胞的增殖。从本研究结果来看，人类牙源性肿瘤和囊肿的发生和生长可能与TGF- α的作用有很大关系。

The purpose of the present study was to investigate histologically and immunohistochemically (BrdU) the effects of growth factor TGF- alpha and carcinogenic heterocyclic amine Trp-P-2 and MelQx on the mouse tooth-germ in organ culture. The mouse tooth-germs were divided into the following 3 experiments (I, II, III) with 7 groups. Experiment I.Group I-A (BGJb) and Group I-B (TGF- alpha). Experiment II.Group II-A (DMSO), Group II-B (Trp-P-2) and Group II-C (MeIQx). Experiment III.Group III-A(TGF- alpha+Trp-P-2) and Group III-B(TGF- alpha+MeIQx).The results of the present study indicated that TGF- alpha promoted markedly the proliferation of epithelial cells of the mouse tooth-germ , but also suggested that TGF- alpha did not stimulate the differentiation of these epithelial cells. Moreover, it was also suggested that Trp-P-2 and MeIQx suppressed the proliferation of these epithelial cells. It seems from the results of the present study that the development and growth of odontogenic tumors and cysts in humans might be related considerably to the action of TGF- alpha.