Effects of TGF-alpha, Trp-P-2 and MeIQx on the Tooth-germ in Organ Culture

本研究的目的是在器官培养中观察生长因子转化生长因子-α、致癌杂环胺Trp-P-2和MelQx对小鼠牙胚发育的影响。小鼠牙胚分为3个实验(I、II、III)，共7组。实验I.实验I-A组(补肾健脾颗粒)和I-B组(转化生长因子-α)。实验II组II-A(DMSO)、II-B组(Trp-P-2)和II-C组(MeIQx)。实验III.实验III-A组(转化生长因子-α+Trp-P-2)和组III-B(转化生长因子-α+MeIQx)。本研究结果表明，转化生长因子-α能显著促进小鼠牙胚上皮细胞的增殖，但也提示转化生长因子-α不能促进这些上皮细胞的分化。此外，Trp-P-2和MeIQx对这些上皮细胞的增殖也有抑制作用。从目前的研究结果看，牙源性肿瘤和牙源性囊肿的发生和生长可能与转化生长因子-α的作用有很大关系。

The purpose of the present study was to investigate histologically and immunohistochemically (BrdU) the effects of growth factor TGF- alpha and carcinogenic heterocyclic amine Trp-P-2 and MelQx on the mouse tooth-germ in organ culture. The mouse tooth-germs were divided into the following 3 experiments (I, II, III) with 7 groups. Experiment I.Group I-A (BGJb) and Group I-B (TGF- alpha). Experiment II.Group II-A (DMSO), Group II-B (Trp-P-2) and Group II-C (MeIQx). Experiment III.Group III-A(TGF- alpha+Trp-P-2) and Group III-B(TGF- alpha+MeIQx).The results of the present study indicated that TGF- alpha promoted markedly the proliferation of epithelial cells of the mouse tooth-germ , but also suggested that TGF- alpha did not stimulate the differentiation of these epithelial cells. Moreover, it was also suggested that Trp-P-2 and MeIQx suppressed the proliferation of these epithelial cells. It seems from the results of the present study that the development and growth of odontogenic tumors and cysts in humans might be related considerably to the action of TGF- alpha.