The in vitro and in vivo study on effects of growth factor on tooth germs

1 .本研究采用A组（对照）、B组（DMSO）、C组（TGF-α）、D组（Trp-P-2）和E组（TGF-α + Trp-P-2） 5组小鼠，研究生长因子TGF-α和致癌物trp -2对移植到裸鼠皮下的器官培养的ddY小鼠牙胚发育的组织学、免疫组织化学（PCNA）和组织学影响。组织学上，C、D、E组牙源性上皮细胞增生明显。D组和E组牙源性上皮细胞出现角化，c组未见角化，E组牙源性上皮细胞区可见囊肿样间隙。免疫组化结果显示，C组、D组和E组牙源性上皮细胞的pcna阳性明显高于a组。免疫组化结果显示，C组和E组牙源性上皮细胞的pcna阳性明显高于a组。生长因子TGF-α和致癌物trp - p- 2 - ii可促进移植器官培养的小鼠牙源性上皮细胞形成囊肿样间隙。本研究采用A组（Control）、B组（DMSO）、C组（TGF-α）、D组（MeIQx）和E组（MeIQx + TGF-α） 5组小鼠，采用组织学和免疫组织化学（PCNA）方法研究致癌物MeIQx和生长因子TGF-α对器官培养的ddY小鼠牙胚发育的影响。从组织学上看，A组和B组的牙胚大小相似。C组牙胚比A、b组大，D、E组牙胚比C组小，C、D、E组牙源性上皮细胞增生明显。免疫组化结果显示，B、C、F、G组成牙上皮细胞pcna阳性明显高于a组。本研究结果提示，致癌物MeIQx和生长因子TGF-α.III促进了小鼠器官培养牙胚中成牙上皮细胞的增殖。本研究旨在探讨生长因子TGF-α和致癌物ENU对ddY小鼠移植器官培养牙胚发育的影响。将从ddY小鼠第17天胚中取出的器官培养牙胚按培养条件分为5组：a组（对照组）、b组（DMSO）、c组（TGF-α）、d组（ENU）、e组（TGF-α + ENU）。将这些牙胚移植到裸鼠皮下组织，移植2周后对移植器官培养的牙胚进行组织学和免疫组织化学（PCNA染色）检查。c、d、e组移植器官培养的牙胚中可见成牙上皮细胞增生。c组和e组牙源性上皮细胞区可见囊肿样间隙。s、e组成牙上皮细胞pcna阳性明显高于a组。本研究结果表明，TGF-α和ENU显著促进移植器官培养牙胚中成牙上皮细胞的增殖

I. In the present study, the effects of both growth factor TGF-α and carcinogen Trp-P-2 on the development of organ-cultured ddY mouse tooth-germ which was transplanted into the subcutaneous tissue of nude mice were investigated histologically, immnohistochemically (PCNA) and histometrically in 5 groups of mice, namely Group A (Control), Group B (DMSO), Group C (TGF-α), Group D (Trp-P-2) and Group E (TGF-α + Trp-P-2). Histologically, in Groups C, D and E, marked proliferation of odontogenic epithelial cells was observed. In Groups D and E, keratinization of the odontogenic epithelial cells was found, but it was not observed in Group C.Moreover, in Group E, a cyst-like space was observed in the area of odontogenic epithelial cells. Immunohistochemically, in Groups C, D and E, PCNA-positivities of the odontogenic epithelial cells were significantly higher than those of Group A.Histometrically, in Groups C and E, the size of the tooth germ was significantly larger in size than that of Gro … More up A. The results of the present study suggest that proliferation, keratinization, cyst-like space formation of odontogenic epitherial cells of transplanted organ-cultured tooth-germs in mice were promoted by growth factor TGF-α and carcinogen Trp-P-2.II. In the present study, the effects of both carcinogen MeIQx and growth factor TGF-α on the development of organ-cultured ddY mouse tooth-germs were investigated histologically and immnohistochemically (PCNA) in 5 groups of mice, namely Group A (Control), Group B (DMSO), Group C (TGF-α), Group D (MeIQx) and Group E (MeIQx + TGF-α). Histologically, in Groups A and B, both tooth-germs appeared in similar size. In Group C, the tooth-germ was bigger in size than those of Groups A and B. In Groups D and E, the tooth-germs were smaller than in Group C. In Groups C, D and E, marked proliferation of odontogenic epithelial cells was observed. Immunohistochemically, in Groups B, C, F and G, PCNA-positivities of odontogenic epithelial cells were significantly higher than those in Group A. The results of the present study suggested that the proliferation of odontogenic epitherial cells in organ-cultured tooth-germs in mice was promoted by carcinogen MeIQx and growth factor TGF-α.III. The purpose of the present study was to investigate the effects of growth factor TGF-α and carcinogenen ENU on the development of the transplanted organ-cultured tooth germs in ddY mice. The organ-cultured tooth-germs removed from the 17th day embryo of the ddY mouse were divided into the following 5 groups according their cultured conditions, Group a (Control), Group b (DMSO), Group c (TGF-α), Group d (ENU), and Group e (TGF-α + ENU). These tooth-germs were transplanted into the subcutaneous tissue of the nude mice, and the transplanted organ-cultured tooth-germs were examined histologically and immunohistochemically (PCNA stainings) 2 weeks after transplantation. In Groups c, d and e, proliferation of odontogenic epithelial cells was found in the transplanted organ-cultured tooth-germ. Moreover, in Groups c and e, a cyst-like space was observed in the area of odontogenic epithelial cells. In groups s and e, PCNA-positivities of odontogenic epithelial cells were significantly higherthan that of Group a. The results of the present study indicated that TGF-α and ENU promoted markedly proliferation of odontogenic epithelial cells in the transplanted organ-cultured tooth-germ Less