The in vitro and in vivo study on effects of growth factor on tooth germs

生长因子对牙胚影响的体内外研究

• 批准号: 18592021
• 负责人: KAMEYAMA Yoichiro
• 金额: $ 2.55万
• 依托单位: Aichi Gakuin University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18592021/
• 关键词: animals; iawbone; tooth germ; organ culture; heterocyclic amine; growth factor

I. In the present study, the effects of both growth factor TGF-α and carcinogen Trp-P-2 on the development of organ-cultured ddY mouse tooth-germ which was transplanted into the subcutaneous tissue of nude mice were investigated histologically, immnohistochemically (PCNA) and histometrically in 5 groups of mice, namely Group A (Control), Group B (DMSO), Group C (TGF-α), Group D (Trp-P-2) and Group E (TGF-α + Trp-P-2). Histologically, in Groups C, D and E, marked proliferation of odontogenic epithelial cells was observed. In Groups D and E, keratinization of the odontogenic epithelial cells was found, but it was not observed in Group C.Moreover, in Group E, a cyst-like space was observed in the area of odontogenic epithelial cells. Immunohistochemically, in Groups C, D and E, PCNA-positivities of the odontogenic epithelial cells were significantly higher than those of Group A.Histometrically, in Groups C and E, the size of the tooth germ was significantly larger in size than that of Gro … More up A. The results of the present study suggest that proliferation, keratinization, cyst-like space formation of odontogenic epitherial cells of transplanted organ-cultured tooth-germs in mice were promoted by growth factor TGF-α and carcinogen Trp-P-2.II. In the present study, the effects of both carcinogen MeIQx and growth factor TGF-α on the development of organ-cultured ddY mouse tooth-germs were investigated histologically and immnohistochemically (PCNA) in 5 groups of mice, namely Group A (Control), Group B (DMSO), Group C (TGF-α), Group D (MeIQx) and Group E (MeIQx + TGF-α). Histologically, in Groups A and B, both tooth-germs appeared in similar size. In Group C, the tooth-germ was bigger in size than those of Groups A and B. In Groups D and E, the tooth-germs were smaller than in Group C. In Groups C, D and E, marked proliferation of odontogenic epithelial cells was observed. Immunohistochemically, in Groups B, C, F and G, PCNA-positivities of odontogenic epithelial cells were significantly higher than those in Group A. The results of the present study suggested that the proliferation of odontogenic epitherial cells in organ-cultured tooth-germs in mice was promoted by carcinogen MeIQx and growth factor TGF-α.III. The purpose of the present study was to investigate the effects of growth factor TGF-α and carcinogenen ENU on the development of the transplanted organ-cultured tooth germs in ddY mice. The organ-cultured tooth-germs removed from the 17th day embryo of the ddY mouse were divided into the following 5 groups according their cultured conditions, Group a (Control), Group b (DMSO), Group c (TGF-α), Group d (ENU), and Group e (TGF-α + ENU). These tooth-germs were transplanted into the subcutaneous tissue of the nude mice, and the transplanted organ-cultured tooth-germs were examined histologically and immunohistochemically (PCNA stainings) 2 weeks after transplantation. In Groups c, d and e, proliferation of odontogenic epithelial cells was found in the transplanted organ-cultured tooth-germ. Moreover, in Groups c and e, a cyst-like space was observed in the area of odontogenic epithelial cells. In groups s and e, PCNA-positivities of odontogenic epithelial cells were significantly higherthan that of Group a. The results of the present study indicated that TGF-α and ENU promoted markedly proliferation of odontogenic epithelial cells in the transplanted organ-cultured tooth-germ Less

I.本研究采用组织学、免疫组化和组织计量学方法，观察了生长因子TGF-α和致癌物Trp-P-2对ddY小鼠牙胚移植到裸鼠皮下的影响，共分5组，即A组（对照组）、B组（DMSO组）、C组（TGF-α组）、D组（Trp-P-2）; E组（TGF-α + Trp-P-2）。组织学上，C、D、E组牙源性上皮细胞增生明显。在D组和E组中，牙源性上皮细胞角化，但在C组中未观察到。此外，在E组中，在牙源性上皮细胞区域中观察到囊肿样间隙。免疫组化结果显示，C、D、E组牙源性上皮细胞PCNA阳性率均显著高于A组。组织学结果显示，C、E组牙胚的大小均显著大于G组。 ...更多信息 上A。本研究结果提示，生长因子TGF-α和致癌物Trp-P-2可促进小鼠牙胚移植后牙源性上皮细胞的增殖、角化和囊样间隙形成。本研究采用组织学和免疫组织化学方法观察了致癌物MeIQx和生长因子TGF-α对ddY小鼠牙胚发育的影响。组织学上，A、B组牙胚大小相似。C组牙胚体积大于A、B组。D、E组牙胚较C组小。C、D、E组牙源性上皮细胞增生明显。免疫组化结果显示，B、C、F、G组牙源性上皮细胞PCNA阳性率均显著高于A组。本研究结果提示，致癌物MeIQx和生长因子TGF-α. III可促进小鼠牙胚器官培养中牙源性上皮细胞的增殖。本研究旨在探讨生长因子TGF-α和致癌因子ENU对ddY小鼠牙胚移植后生长发育的影响。将ddY小鼠17天胚胎的牙胚器官培养物按其培养条件分为以下5组：a组（对照）、B组（DMSO）、c组（TGF-α）、d组（ENU）和e组（TGF-α + ENU）。将这些牙胚移植到裸鼠的皮下组织中，并在移植后2周对移植的器官培养的牙胚进行组织学和免疫组织化学（PCNA染色）检查。c、d、e组移植的牙胚组织中可见牙源性上皮细胞增殖。此外，在c组和e组中，在牙源性上皮细胞区域中观察到囊肿样间隙。s组和e组牙源性上皮细胞PCNA阳性率明显高于a组。结果表明，TGF-α和ENU对牙胚移植后牙源性上皮细胞的增殖有明显的促进作用

项目成果

Histological and Immunohistochemical Study on Effects of TGF-α and Trp-P-2 on the Transplanted Organ-Cultured Mouse Tooth-germ

TGF-α和Trp-P-2对移植器官培养小鼠牙胚影响的组织学和免疫组织化学研究

• 发表时间: 2006
• 期刊: Journal of Oral Medicine and Pathology 11
• 作者: Yoshihiko Sugita;Katsutoshi Kubo;Emiko Sato;Hatsuhiko Maeda;Yoichiro Kameyama
• 通讯作者: Yoichiro Kameyama

Transforming Growth Eactor-α とN-ethy1-N-nitrosoureaが器官培養のマウス移植歯胚の発育におよぼす影響

转化生长因子-α和N-乙基-N-亚硝基脲对器官培养小鼠移植牙胚发育的影响

• 发表时间: 2008
• 期刊: 愛知学院大学歯学会誌 (In press)
• 作者: 小澤 学;杉田 好彦;佐藤 恵美子;久保 勝俊;神野 正人;高山 光平;亀山 洋一郎;前田 初彦
• 通讯作者: 前田 初彦

TGF-αとENUがマウスの移植器官培養歯胚の発育におよぼす影響

TGF-α和ENU对小鼠移植器官培养牙胚发育的影响

• 发表时间: 2008
• 期刊: 愛知学院大学歯学会誌
• 作者: 小澤 学;竹内佐年;吉田憲生;杉田好彦;久保勝俊;佐藤恵美子;前田初彦;亀山洋一郎
• 通讯作者: 亀山洋一郎

Effects of Heterocyclic Amine MeIQx and Growth Factor TGF-α on the Development of Organ-Cultured Mouse Tooth-germ-Histological and Immunohistochemical Study-

杂环胺MeIQx和生长因子TGF-α对器官培养小鼠牙胚发育的影响-组织学和免疫组织化学研究-

• 发表时间: 2007
• 期刊: The Aichi-Gakuin Journal of Dental Science(Japanese) 45
• 作者: Nobue Hirai;Yoshihiko Sugita;Katsutoshi Kubo;Emiko Sato;Hatsuhiko Maeda;Yoichiro Kameyama
• 通讯作者: Yoichiro Kameyama

Effects of Transforming Growth Factor-α and N-ethyl-N-nitrosourea on the Development of Transplanted Organ-Cultured Mouse Tooth-germ

转化生长因子-α和N-乙基-N-亚硝基脲对移植器官培养小鼠牙胚发育的影响

• 发表时间: 2008
• 期刊: The Aichi-Gakuin Journal of Dental Science(Japanese) (in press)
• 作者: Manabu Ozawa;Yoshihiko Sugita;Emiko Sato;Katsutoshi Kubo;Masato Jinno;Mitsuhiro Takayama;Yoichiro Kameyama;Hatsuhiko Maeda
• 通讯作者: Hatsuhiko Maeda