A Study on development mechanism of Odontogenic Tumor

探讨牙源性囊肿和牙源性肿瘤发生的病理机制，以及致癌物Trp-P-2、MeIQx和生长因子TGF-α对小鼠牙胚的影响。实验一采用组织学和免疫组化方法研究Trp-P-2和TGF-α单独及联合应用对ddY胚胎鼠磨牙牙胚细胞增殖和分化的影响。TGF-α促进牙胚细胞增殖，但抑制其分化。Trp-P-2抑制牙胚细胞的增殖。实验二：MeIQx和TGF-α单独及联合应用对小鼠磨牙牙胚细胞增殖和分化的影响。MeIQx和TGF-α均能促进移植牙胚细胞增殖。实验三：Trp-P-2和TGF-α单独及联合应用对小鼠磨牙牙胚细胞增殖和分化的影响。Trp-P-2联合TGF-α可促进牙胚上皮细胞增殖，表现为角化和囊肿形成。本研究结果提示，某些致癌物质和生长因子参与了牙源性囊肿和牙源性肿瘤的发生发展。

To investigate the pathological mechanism of the development of odontogenic cyst and odontogenic tumor, the effects of carcinogens, Trp-P-2 and MeIQx, and growth factor TGF-α on the mouse tooth-germ in vitro and in vivo. In experiment I, effects of Trp-P-2 and TGF-α , alone and in combination, on the cell proliferation and differentiation of the organ-cultured molar tooth-germ of ddY embryonic mouse were studied histologically and immunohisochemically (PCNA). TGF-α increased the cell proliferation of the organ-cultured tooth-germ, but inhibited its differentiation. Trp-P-2 inhibited the cell proliferation of the organ-cultured tooth-germ. In experiment II, effects of MeIQx and TGF-α, alone and in combination, on the cell proliferation and differentiation of the mouse molar tooth-germs, which were transplanted into BALB/C nude mouse following organ-culture. Both of MeIQx and TGF-α increased the cell proliferation of the transplanted mouse tooth-germ. In experiment III, effects of Trp-P-2 and TGF-α, alone and in combination, on the cell proliferation and differentiation of the mouse molar tooth-germs, which were transplanted into BALB/C nude mouse following organ-culture. Trp-P-2 in combination with TGF-α increased the cell proliferation of the odontogenic epithelium of the transplanted tooth-germs, which showed keratinization and cyst formation. The results of the present study may suggest that some carcinogens and growth factor are involved in the development of odontogenic cyst and odontogenic tumor.