A Study on development mechanism of Odontogenic Tumor

牙源性肿瘤发生机制的研究

• 批准号: 13671927
• 负责人: KAMEYAMA Yoichiro
• 金额: $ 1.98万
• 依托单位: Aichi-Gakuin University School of Dentistry
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671927/
• 关键词: animal; jaw bone; tooth germ; organ culture; heterocyclic amine; growth factor

To investigate the pathological mechanism of the development of odontogenic cyst and odontogenic tumor, the effects of carcinogens, Trp-P-2 and MeIQx, and growth factor TGF-α on the mouse tooth-germ in vitro and in vivo. In experiment I, effects of Trp-P-2 and TGF-α , alone and in combination, on the cell proliferation and differentiation of the organ-cultured molar tooth-germ of ddY embryonic mouse were studied histologically and immunohisochemically (PCNA). TGF-α increased the cell proliferation of the organ-cultured tooth-germ, but inhibited its differentiation. Trp-P-2 inhibited the cell proliferation of the organ-cultured tooth-germ. In experiment II, effects of MeIQx and TGF-α, alone and in combination, on the cell proliferation and differentiation of the mouse molar tooth-germs, which were transplanted into BALB/C nude mouse following organ-culture. Both of MeIQx and TGF-α increased the cell proliferation of the transplanted mouse tooth-germ. In experiment III, effects of Trp-P-2 and TGF-α, alone and in combination, on the cell proliferation and differentiation of the mouse molar tooth-germs, which were transplanted into BALB/C nude mouse following organ-culture. Trp-P-2 in combination with TGF-α increased the cell proliferation of the odontogenic epithelium of the transplanted tooth-germs, which showed keratinization and cyst formation. The results of the present study may suggest that some carcinogens and growth factor are involved in the development of odontogenic cyst and odontogenic tumor.

目的：探讨牙源性囊肿和牙源性肿瘤发生的病理机制，以及致癌物、Trp-P-2、MeIQx、生长因子TGF-α对小鼠牙胚的体外和体内影响。实验一采用组织学和免疫组化（PCNA）方法，研究了Trp-P-2和TGF-α单独及联合作用对器官培养的ddY胚胎小鼠磨牙胚细胞增殖和分化的影响。TGF-α促进器官培养牙胚细胞增殖，抑制其分化。Trp-P-2对器官培养牙胚的细胞增殖有抑制作用。实验二：meqx和TGF-α单独及联合作用对BALB/C裸鼠器官培养后移植的小鼠磨牙胚细胞增殖分化的影响。MeIQx和TGF-α均能促进移植小鼠牙胚细胞增殖。实验三：Trp-P-2和TGF-α单独及联合作用对BALB/C裸鼠磨牙胚移植后细胞增殖和分化的影响。Trp-P-2联合TGF-α可促进移植牙胚成牙上皮细胞增殖，出现角化和囊肿形成。本研究结果提示，某些致癌物和生长因子参与了牙源性囊肿和牙源性肿瘤的发生发展。