Study of Experimental Oral Tumors Induced by Mutagenic Heterocyclic Amines in Boiled Food

本研究的目的是观察Trp-P-2（3-氨基-l-甲基- 5h -吡啶[4,3-b]吲哚）治疗两次舌上皮细胞的组织学和超微结构变化。光显微法将仓鼠分为10组（A-J组）。在所有10组中，用剪刀切除舌尖（2mm）。A、F组创面每周涂DMSO（二甲基硫氧化物）3次，连续8周。B组、C组、D组、G组、H组、I组创面用0.5%的Trp-P-2溶液涂抹，每周3次，连续8周。E组、J组创面8周不处理。8周结束时，A、B、C、D、E组再次切除舌尖，F、G、H、I、J组不切除舌尖。在A、C、F和H组，每天用DRSO涂舌头，直到动物被杀死。在B组和G组中，动物在被杀死之前不接受任何治疗。在D组、E组、l组和J组中，每天在舌头上涂上色氨酸- p- 2，直到动物被杀死。各组分别于第二次切除后11、15天或8周后处死。对舌头做了组织学检查。a、c、d组分别给予与a、c、d组相同的处理。这些动物在第二次切除后15天被杀死。对舌头进行了超结构检查。1) A组在11和15 d时，舌部仅出现角化过度。2) B组，11、15 d时舌上皮轻度发育不良。3) C组和D组11、15 D时，舌部上皮发育异常明显。这些变化包括细胞定向障碍、细胞和细胞核多形性、细胞核深染、有丝分裂象数量增加、角化异常和上皮突起伸长。4) E组11、15 d时，舌部上皮轻度发育不良。5) F、G、H、I、J组舌部仅出现角化过度。电镜观察1)a组基底细胞层细胞呈圆形或卵圆形，细胞核位于中央较大；基底细胞下方的基底层几乎完整，但有时缺失或不规则多层。刺细胞层细胞略扁平。这些细胞细胞质中的张力丝束围绕着细胞核或朝向桥粒。颗粒细胞层细胞中等扁平，含有不同数量的角膜透明素颗粒。角化细胞层的细胞非常扁平，没有细胞核。2) c、d组基底细胞层细胞形态不规则。细胞核增大，形状不规则。细胞质中的张力丝束环绕细胞核或呈旋涡状排列。偶见核内管状结构。基底细胞以下的基底层常无或不规则多层。棘细胞层细胞形态不规则。在这些细胞的细胞质中，张力丝束围绕着细胞核或分散或局部或呈旋涡状排列。偶尔在细胞核内观察到病毒样颗粒和核内管状结构。颗粒细胞层的细胞变平。有时，这些细胞的细胞核含有病毒样颗粒。角化细胞层中的细胞通常没有细胞核，但偶尔有带病毒样颗粒的细胞核。少

The purpose of this study was to investigate histologically and ultrastructurally the changes of epithelial cells in the tongue which was wounded twice and treated with Trp-P-2(3-amino-l-metliyl-5H -pyrido[4,3-b]indole).1. Light Microscopy Hamsters were divided into 10 groups(Groups A-J). In all 10 groups, the tip(2mm) of the tongue was excised with scissors. In Groups A and F, the wound was painted trice weekly with DMSO(dimethyl sulf- oxide) for 8 weeks. In Groups B,C,D,G,H and I, the wound was painted trice weekly with a 0.5%solution of Trp-P-2 in DMSO for 8 weeks. In Groups E and J, the wound was not treated for 8 weeks. At the end of 8 weeks, in Groups A,B,C,D and E, the tip of the tongue was again excised, while in Groups F,G,H,I and J, the tip was not excised. In Groups A,C,F and H, the tongue was painted daily with DRSO until the animals were killed. In Groups B and G, the animals received no treatment until they were killed. In Groups D,E,l and J, the tongue was painted daily … More with Trp-P-2 until the animals were killed. Animals of all groups were killed aL 11 and 15 days after the second excision or after the end of 8 week. The tongue was examined histologically.2. Electron Microscopy In Groups a,c and d, the animals received the same treatment as those of Groups A,C and D, respectively. The animals were killed at 15 days after the second excision. The tongue was examined ultra- structurally.3. Light Microscopic Observation 1)In Groups A, at 11 and 15 days, the tongue showed only hyperkeratosis. 2)ln Groups B, at 11 and 15 days, the tongue snowed the changes of the mild epithelial dysplasia. 3)ln Groups C and D, at 11 and 15 days, the tongue showed the changes of the marked epithelial dysplasia. These changes were disorientation of cells, cellular and nuclear pleomorphisms, nuclear hyperchromatism, increased number of mitotic figures, abnormal keratinization and elongation of epithelial projection. 4)In Group E, at 11 and 15 days, the tongue showed the changes of the mild epithelial dysplasia. 5)In Groups F,G,H,I and J, the tongue showed only hyperkeratosis.4. Electron Microscopic Observation 1)In Group a, the cells in the basal cell layer were round or ovoid in shape with large centrally-located nuclei. The basal lamitia just below the basal cells was almost intact, but on occasions absent or irregularly multilayered. The cells in the prickle cell layer were slightly flattened. The bundles of tonofilaments in the cytoplasm of these cells surrounded the nucleus or were oriented towards the desmosomes. The cells in the granular cell layer were moderately flattened and contained various amount of keratohyaline granules. The cells in the keratinized cell layer were very flattened and had no nucleus. 2)In Groups c and d, the cells in the basal cell layer were irregular in shape. The nucleus was enlarged and also appeared irregular in shape. The bundles of tonofilaments in the cytoplasm surrounded the nucleus or showed swirl-like arrangement. Occasionally, intranuclear tubular-like structures were observed within the nucleus. The basal lamina below the basal cells frequently absent or irregularly multilayered. The cells in the prickle cell layer were irregular in shape. In the cytoplasm of these cells, the bundles of tonofilaments surrounded the nucleus or scattered or localized or showed a swirl-like arrangement. Occasionally, virus-like particles and intranuclear tubular-like structures were observed within the nucleus. The cells in the granular cell layer were flattened. Sometimes, the nuclei of these cells contained virus-like particles. The cells in the keratinized cell layer usually lacked a nucleus, but on occasions had a nucleus with virus-like particles. Less