Study of Experimental Oral Tumors Induced by Mutagenic Heterocyclic Amines in Boiled Food

水煮食品中杂环胺诱变诱发实验性口腔肿瘤的研究

• 批准号: 63570863
• 负责人: KAMEYAMA Yoichiro
• 金额: $ 1.41万
• 依托单位: Aichi-Gakuin University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63570863/
• 关键词: Animal; Tongue; Wound; Experimental Oral Tumor; Heterocyclic Amines

The purpose of this study was to investigate histologically and ultrastructurally the changes of epithelial cells in the tongue which was wounded twice and treated with Trp-P-2(3-amino-l-metliyl-5H -pyrido[4,3-b]indole).1. Light Microscopy Hamsters were divided into 10 groups(Groups A-J). In all 10 groups, the tip(2mm) of the tongue was excised with scissors. In Groups A and F, the wound was painted trice weekly with DMSO(dimethyl sulf- oxide) for 8 weeks. In Groups B,C,D,G,H and I, the wound was painted trice weekly with a 0.5%solution of Trp-P-2 in DMSO for 8 weeks. In Groups E and J, the wound was not treated for 8 weeks. At the end of 8 weeks, in Groups A,B,C,D and E, the tip of the tongue was again excised, while in Groups F,G,H,I and J, the tip was not excised. In Groups A,C,F and H, the tongue was painted daily with DRSO until the animals were killed. In Groups B and G, the animals received no treatment until they were killed. In Groups D,E,l and J, the tongue was painted daily … More with Trp-P-2 until the animals were killed. Animals of all groups were killed aL 11 and 15 days after the second excision or after the end of 8 week. The tongue was examined histologically.2. Electron Microscopy In Groups a,c and d, the animals received the same treatment as those of Groups A,C and D, respectively. The animals were killed at 15 days after the second excision. The tongue was examined ultra- structurally.3. Light Microscopic Observation 1)In Groups A, at 11 and 15 days, the tongue showed only hyperkeratosis. 2)ln Groups B, at 11 and 15 days, the tongue snowed the changes of the mild epithelial dysplasia. 3)ln Groups C and D, at 11 and 15 days, the tongue showed the changes of the marked epithelial dysplasia. These changes were disorientation of cells, cellular and nuclear pleomorphisms, nuclear hyperchromatism, increased number of mitotic figures, abnormal keratinization and elongation of epithelial projection. 4)In Group E, at 11 and 15 days, the tongue showed the changes of the mild epithelial dysplasia. 5)In Groups F,G,H,I and J, the tongue showed only hyperkeratosis.4. Electron Microscopic Observation 1)In Group a, the cells in the basal cell layer were round or ovoid in shape with large centrally-located nuclei. The basal lamitia just below the basal cells was almost intact, but on occasions absent or irregularly multilayered. The cells in the prickle cell layer were slightly flattened. The bundles of tonofilaments in the cytoplasm of these cells surrounded the nucleus or were oriented towards the desmosomes. The cells in the granular cell layer were moderately flattened and contained various amount of keratohyaline granules. The cells in the keratinized cell layer were very flattened and had no nucleus. 2)In Groups c and d, the cells in the basal cell layer were irregular in shape. The nucleus was enlarged and also appeared irregular in shape. The bundles of tonofilaments in the cytoplasm surrounded the nucleus or showed swirl-like arrangement. Occasionally, intranuclear tubular-like structures were observed within the nucleus. The basal lamina below the basal cells frequently absent or irregularly multilayered. The cells in the prickle cell layer were irregular in shape. In the cytoplasm of these cells, the bundles of tonofilaments surrounded the nucleus or scattered or localized or showed a swirl-like arrangement. Occasionally, virus-like particles and intranuclear tubular-like structures were observed within the nucleus. The cells in the granular cell layer were flattened. Sometimes, the nuclei of these cells contained virus-like particles. The cells in the keratinized cell layer usually lacked a nucleus, but on occasions had a nucleus with virus-like particles. Less

本研究的目的是从组织学和超微结构上探讨两次损伤后经Trp-P-2(3-amino-l-metliyl-5H-pyrido[4,3-b]indole)处理的舌上皮细胞的变化。 1．光学显微镜检查仓鼠被分为10组（A-J组）。所有10组均用剪刀切除舌尖（2mm）。在 A 组和 F 组中，每周三次用 DMSO（二甲基亚砜）涂抹伤口，持续 8 周。在B、C、D、G、H和I组中，每周三次用0.5％Trp-P-2的DMSO溶液涂抹伤口，持续8周。在E组和J组中，伤口已经8周没有得到处理。 8周后，A、B、C、D、E组再次切除舌尖，F、G、H、I、J组不切除舌尖。在A、C、F和H组中，每天用DRSO涂在舌头上直到动物被处死。在 B 组和 G 组中，动物在被处死之前没有接受任何治疗。在 D、E、l 和 J 组中，每天用 Trp-P-2 涂在舌头上，直到动物被杀死。第二次切除后第11天和第15天或第8周结束后处死所有组的动物。对舌头进行组织学检查。2．电子显微镜检查a、c和d组中的动物分别接受与A、C和D组相同的处理。第二次切除后15天处死动物。对舌头进行超微结构检查。3．光镜观察1)A组在第11天和第15天时，舌头仅显示角化过度。 2)B组第11、15天舌体出现轻度上皮异常增生的变化。 3)C组和D组在第11天和第15天时,舌体出现明显的上皮异常增生的变化。这些变化是细胞定向障碍、细胞和核多形性、核深染、有丝分裂数增加、角化异常和上皮突起伸长。 4)E组第11天和第15天舌体出现轻度上皮不典型增生的变化。 5)F、G、H、I、J组舌仅角化过度。4．电镜观察1)a组基底细胞层细胞呈圆形或卵圆形，细胞核较大，居中。基底细胞下方的基底层几乎完整，但有时缺失或不规则多层。棘细胞层细胞稍扁平。这些细胞的细胞质中的张力丝束围绕着细胞核或朝向桥粒。颗粒细胞层细胞适度扁平，含有不同量的透明角质颗粒。角化细胞层的细胞非常扁平，没有细胞核。 2)c、d组基底细胞层细胞形态不规则。细胞核增大，形状不规则。细胞质中的张力丝束围绕细胞核或呈漩涡状排列。有时，在细胞核内观察到核内管状结构。基底细胞下方的基底层通常不存在或呈不规则多层结构。棘细胞层细胞形状不规则。在这些细胞的细胞质中，张力丝束围绕着细胞核，或分散，或局部，或呈漩涡状排列。偶尔在细胞核内观察到病毒样颗粒和核内管状结构。颗粒细胞层中的细胞被压扁。有时，这些细胞的细胞核含有病毒样颗粒。角化细胞层的细胞通常缺乏细胞核，但有时细胞核内含有病毒样颗粒。较少的