Analysis of developmental genetic system using small teleost fish as vertebrate model systems

以小型硬骨鱼为脊椎动物模型系统的发育遗传系统分析

• 批准号: 12202004
• 负责人: TAKEDA Hiroyuki
• 金额: $ 133.63万
• 依托单位: University of Tokyo (2001-2004)National Institute of Genetics (2000)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12202004/
• 关键词: medaka; genome; EST; Hox; mutant; development; evolution; SNP; SNPS; Hox cluster; Hox; 器官形成; BAC

In the present study, we focused on the medaka that became an attractive model organism for the study of vertebrate early development and genome evolution. The following three groups were jointed, aiming at the establishment of comprehensive resources for medaka developmental genetics as well as structural and functional analyses of developmentally important genes in medaka.1. Takeda and NaruseTo strengthen the study of medaka developmental genetics, Takeda's laboratory has conducted a large-scale isolation of ESTs from medaka embryos and developed tools that facilitate mutant analysis such as rapid mapping system, M-markers and microarray with 8,000 uni-genes. These resources are accelerating medaka mutant analyses and made an important contribution to the medaka genome sequencing project.2. HoriHori's laboratory analyzed the organization of the entire hox gene loci. Together with the information in the Fugu genome database and in the Danio genome database, the physical maps of three fish genomes were constructed and compared one another. Not only numbers of hox genes but also the distances between the neighboring hox genes are highly similar between medaka and fugu. As for six clusters, Aa, Ab, Ba, Bb, Ca and Da that are commonly present in the three fishes, only few or no differences were found in each cluster. Thus, the hox gene sets should have been well conserved once they had been established in respective species.3. KudoKudo's laboratory has screened for mutants that are defective in organogenesis and fin regeneration in the medaka, using ethylnitrosourea (ENU) as a mutagen. A total of 386 mutagenized genomes were scored to identify 151 mutations affecting organogenesis, and 16 mutations affecting the fin regeneration were identified. In parallel, they isolated 20,000 ESTs from re-generating fins, representing about 3,000 uni-genes.

在目前的研究中，我们重点关注青竹，它成为研究脊椎动物早期发育和基因组进化的一个有吸引力的模式生物。以下三个小组联合起来，旨在建立青竹发育遗传学的综合资源，以及青竹发育重要基因的结构和功能分析。武田和那不勒斯为了加强对青竹发育遗传学的研究，武田的实验室从青竹胚胎中大规模分离了EST，并开发了便于突变分析的工具，如快速定位系统、M标记和包含8,000个单基因的微阵列。这些资源正在加速青竹突变分析，并为青竹基因组测序项目做出了重要贡献。HoriHori的实验室分析了整个Hox基因座的组织。结合河豚基因组数据库和Danio基因组数据库中的信息，构建了三种鱼类基因组的物理图谱，并进行了比较。青竹和河豚不仅HOX基因数目相似，而且相邻HOX基因之间的距离也高度相似。对于三种鱼中普遍存在的AA、AB、BA、BB、Ca和Da 6个类群，每个类群的差异很小或没有差异。因此，HOX基因组一旦在各自的物种中建立起来，就应该得到很好的保存。工藤的实验室使用乙基亚硝脲(ENU)作为诱变剂，筛选出在青竹器官发生和鳍再生方面存在缺陷的突变株。总共对386个突变基因组进行了评分，鉴定了151个影响器官发生的突变，并鉴定了16个影响鳍再生的突变。与此同时，他们从再生的鳍中分离出20,000个EST，代表大约3,000个单基因。

项目成果

Matsuda, M.: "DMY is a Y-specific DM-domain gene required for male development in the medaka fish."Nature. 417. 559-563 (2002)

Matsuda, M.：“DMY 是青鳉鱼雄性发育所需的 Y 特异性 DM 结构域基因。”《自然》。

Early fin primordia of zebrafish larvae regenerate by a similar growth control mechanism with adult regeneration

• DOI: 10.1002/dvdy.20181
• 发表时间: 2004-12-01
• 期刊: DEVELOPMENTAL DYNAMICS
• 影响因子: 2.5
• 作者: Kawakami, A;Fukazawa, T;Takeda, H
• 通讯作者: Takeda, H

Takuya Sakaguchi: "Expression of zebrafish BTG-b, anti-proliferative cofactor, during early embryogenesis"Mechanisms of Development. (in press). (2001)

Takuy​​a Sakaguchi：“斑马鱼 BTG-b（抗增殖辅因子）在早期胚胎发生过程中的表达”发育机制。

Naruse, K.: "Medaka genomics : a bridge between mutant phenotypes and gene function"Mechanisms of Development. (印刷中). (2004)

Naruse, K.：“青鳉基因组学：突变表型和基因功能之间的桥梁”发展机制（2004 年出版）。

Sakaguchi, T: "Formation and patterning roles of the yolk syncytial layer. (Review)"Pattern Formation in Zebrafish. 1-14 (2002)

Sakaguchi，T：“卵黄合胞体层的形成和图案形成作用。（评论）”斑马鱼的图案形成。