Identification of aging-related factors that facilitate Alzheimer's disease

1.磷脂酰肌醇蛋白聚糖-1（glypican-1）是一种Aβ结合蛋白。磷脂酰肌醇蛋白聚糖-1富集在筏馏分，并沉积在老年斑。磷脂酰肌醇蛋白聚糖-1增强Aβ诱导的细胞死亡。2.利用PS1野生型和突变型细胞株的差异显示，我们鉴定了一种新的蛋白Adoplin。Applin是一种膜蛋白，与PS1形成复合物。利用酵母双杂交系统，我们鉴定了一种新的Aβ结合蛋白AB-DIP，该蛋白含有CARD和核靶向序列。AB-DIP自身过表达可诱导细胞死亡，并增强Aβ诱导的细胞死亡。4.将Neprilysin基因敲除小鼠与突变型PS 1转基因小鼠杂交。小鼠没有显示出阿尔茨海默氏症表型的增强效应。

1.We identified glypican-1 as an Aβ-binding protein. Glypican-1 was enriched in the raft fraction, and deposited in senile plaques. Glypican-1 enhanced Aβ-induced cell death.2.By using the differential display between PS1 wild and mutant cell lines, we identified a novel protein, adoplin. Adoplin was a membrane protein, and made a complex with PS1. Over expression of adoplin enhanced Aβ production.3.By using the yeast two-hybrid system, we identified a novel Aβ-binding protein, AB-DIP, which contained CARD and nuclear targeting sequence. Overe-xpression of AB-DIP itself induced cell death and enhanced Aβ- induced cell death.4.Neprilysin-knock-out mice were crossed with mutant PS1-transgenic mice. The mice did not show an enhancing effect of the Alzheimer phenotype.