mRNA localization

mRNA定位在真核生物基因表达的时空调控中起着重要作用。使用斑马鱼作为模式生物，我们成功地展示了以下几点：（i）含有局部mRNA（如vasa、nanosl和dazl）的母体供应的细胞质在斑马鱼中起生殖细胞决定因子的作用，（ii）斑马鱼“生殖质”mRNA在早期卵子发生期间经由线粒体云运输到植物极，（iii）未定位的nanosl和tdrd 7 mRNA在体细胞中通过大量表达的微小RNA之一miR-430的去腺苷酸化或聚（A）尾缩短而受到增强的去稳定化和翻译抑制，以及（iv）miR-430在斑马鱼胚胎中抑制母体提供的mRNA，在母体到合子的转变中起关键作用。

mRNA localization plays important roles in spatio-temporal control of eukaryotic gene expression. Using zebrafish as a model organism, we succeed to show the following points: (i) the maternally supplied cytoplasm containing localized mRNAs, such as vasa, nanosl and dazl, functions as the germ cell determinant in zebrafish, (ii) the zebrafish "germ plasm" mRNAs are transported to the vegetal pole via mitochondrial clouds during early oogenesis, (iii) unlocalized nanosl and tdrd7 mRNAs are subjected to enhanced destabilization and translation repression via deadenylation or poly(A) tail shortening by one of the abundantly expressed microRNA, miR-430, in somatic cells, and also (iv) miR-430 plays a key role in maternal-to-zygotic transition, repressing maternally supplied mRNAs in zebrafish embryos.