mRNA localization

mRNA定位

• 批准号: 14035237
• 负责人: INOUE Kunio
• 金额: $ 43.2万
• 依托单位: Kobe University (2003-2006)Nara Institute of Science and Technology (2002)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14035237/
• 关键词: zabrafish; germ plasm; mRNA localization; germ cell; micro RNA; translational regulation; microRNA(miRNA); ミトコンドリア凝集体; 母性mRNA; ポリA鎖; RNA情報発現; 発生分化; RNA結合蛋白質; mRNP

mRNA localization plays important roles in spatio-temporal control of eukaryotic gene expression. Using zebrafish as a model organism, we succeed to show the following points: (i) the maternally supplied cytoplasm containing localized mRNAs, such as vasa, nanosl and dazl, functions as the germ cell determinant in zebrafish, (ii) the zebrafish "germ plasm" mRNAs are transported to the vegetal pole via mitochondrial clouds during early oogenesis, (iii) unlocalized nanosl and tdrd7 mRNAs are subjected to enhanced destabilization and translation repression via deadenylation or poly(A) tail shortening by one of the abundantly expressed microRNA, miR-430, in somatic cells, and also (iv) miR-430 plays a key role in maternal-to-zygotic transition, repressing maternally supplied mRNAs in zebrafish embryos.

MRNA定位在真核基因表达的时空调控中起着重要作用。以斑马鱼为模型生物，我们成功地证明了以下几点：(I)在斑马鱼中，母体提供的含有局部mRNAs的细胞质，如VasA，Nanos1和DAZL，起着生殖细胞决定因素的作用；(Ii)斑马鱼的“生殖质”mRNAs在卵子发生的早期通过线粒体云运输到植物极；(Iii)未定位的Nanos1和tdrd7mRNAs通过死基化或在体细胞中通过多聚(A)尾巴缩短而受到加强的失稳和翻译抑制；(Iv)miR-430在母体细胞到合子的转变中起关键作用抑制斑马鱼胚胎中母体提供的mRNAs。

项目成果

S.Maegawa: "Zebrafish DAZ-like protein controls translation via the sequence 'GUUC'"Genes Cells. 7. 971-984 (2002)

S.Maekawa：“斑马鱼 DAZ 样蛋白通过序列‘GUUC’控制翻译”Genes Cells。

The formation of primordial germ cells from germline cells in spherical embryos derived from the blastodisc of 2-cell embryos in goldfish, Carassius auratus.

源自金鱼 2 细胞胚胎胚盘的球形胚胎中的生殖细胞形成原始生殖细胞。

• 发表时间: 2005
• 期刊: International Journal of Developmental Biology 49
• 作者: Fujimoto T;Kataoka T;Sakao S;Saito T;Yamaha E;Arai K;Murakami M;Otani S
• 通讯作者: Otani S

TAP/NXF1,the primary mRNA export receptor, specifically interacts wlth a neuronal RNA-binding protein HuD.

TAP/NXF1 是主要 mRNA 输出受体，与神经元 RNA 结合蛋白 HuD 发生特异性相互作用。

• 发表时间: 2004
• 期刊: Biochem.Biophys.Res.Commun. 321
• 作者: Saito;K.
• 通讯作者: K.

Hashimoto, Y.: "Requirement of localized maternal factors for zebrafish germ cell formation."Dev.Biol.. (印刷中).

Hashimoto, Y.：“斑马鱼生殖细胞形成对局部母体因素的要求。”Dev.Biol..（正在出版）。

Hayakawa, T.: "Cell cycle behavior of human HP1 subtypes : distinct molecular domains of HP1 are required for their centromeric localization during interphase and metaphase."J.Cell Sci.. 116. 3327-3338 (2003)

Hayakawa, T.：“人类 HP1 亚型的细胞周期行为：HP1 的不同分子结构域是间期和中期着丝粒定位所必需的。”J.Cell Sci.. 116. 3327-3338 (2003)