Mechanism of U1 snRNP-independent splicing

许多真核生物基因被内含子打断，内含子通过RNA剪接从初级转录本中移除。在剪接过程中，U1 snRNP在5'剪接位点识别中起着至关重要的作用。最近，我们在人类中发现了第一个自然发生的不依赖于u1的u2型剪接的例子。我们目前的研究表明，如果内含子9的5'剪接位点突变为依赖于u1的类型，组织特异性剪接调节剂Fox-1无法诱导hf1 γ外显子9跳变，这表明不依赖于u1的剪接有助于调节一类pre- mrna的选择性剪接。

Many eukaryotic genes are interrupted by introns that are removed from primary transcripts by RNA splicing. U1 snRNP plays a crucial role in the 5' splice site recognition during splicing. Recently, we found the first example of naturally-occurring U1-independent U2-type splicing in humans. Our present study showed that a tissue-specific splicing regulator, Fox-1, failed to induce hF1γexon 9 skipping if the 5' splice site of intron 9 was mutated to the U1-dependent type, suggesting that U1-independent splicing contributes to the regulation of alternative splicing of a class of pre-mRNAs.