Real-World Modeling of Self-Emergent Active Cell

自生活性细胞的真实世界建模

• 批准号: 17076007
• 负责人: 吉川 研一
• 金额: $ 55.87万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17076007/
• 关键词: リポソーム; 人工細胞モデル; その場観察; DNA折り畳み転移; 自己組織化; ナノ構造制御; 油中水滴; レーザーピンセット; ミクロ液的; ミクロ液滴

The main purpose of this project is the construction of "non-equilibrium dissipative"artificial cell models. To achieve the purpose, the following three axes are employed; (i) in vitro re-construction of chromosome, (ii) transcriptional regulation by DNA coil-globule transition, (iii) cell-sized space specificity in the dynamics of membrane, DNA, and protein. The representative results in relation to the cell engineering are as follows; (i) Establishment of the methodology to entrap bio-polymers and substrates in a cell-sized model system. (ii) Unraveling of the scenario on the higher-order structural transition of chromatin. (iii) Invention of the technique of noninvasive and noncontact manipulation of bio-macromolecules by laser. Main results on basic science are; (i) Clear experimental representation on the confined effect of cell-sized space was demonstrated. For example, actin exhibits the two-step transition in a confined space, whereas it shows single step transduction in bulk phase. (ii) Clear evidence was shown on the cross-talk between membrane fluidity and the bulk viscosity.

本计画的主要目的是建构“非平衡耗散”人工细胞模型。为了实现这一目的，采用了以下三个轴：（i）染色体的体外重建，（ii）通过DNA卷曲-小球转换的转录调控，（iii）膜，DNA和蛋白质动力学中的细胞大小空间特异性。与细胞工程相关的代表性结果如下：（i）在细胞大小的模型系统中捕获生物聚合物和底物的方法的建立。(ii)染色质高级结构转变的研究进展。(iii)发明了激光对生物大分子的非侵入性和非接触性操作技术。在基础科学方面的主要研究结果有：（1）明确了细胞大小空间的限制效应的实验表征。例如，肌动蛋白在有限空间中表现出两步转变，而在体相中表现出单步转导。(ii)在膜流动性和体积粘度之间的串扰上显示出明确的证据。

项目成果

Topoisomerase II, a scaffold component, promotes chromatin-compaction in vitro in a linker-histone H1-dependent manner

拓扑异构酶 II 是一种支架成分，以连接组蛋白 H1 依赖性方式促进体外染色质压缩

• 发表时间: 2007
• 期刊: Nucleic Acids Research 35
• 作者: K. Hizume;S. Araki;K. Yoshikawa and K. Takeyasu
• 通讯作者: K. Yoshikawa and K. Takeyasu

Lively dynamic pattern formation in an active liquid film

在活性液膜中形成生动的动态图案

• 发表时间: 2009
• 作者: 陳永軍;長峯祐子、吉川研
• 通讯作者: 長峯祐子、吉川研

Bundle Formation in Crowded Environment with Giant DNA and F-Actin inside a Micro-Meter Scale Closed-Space

微米级封闭空间内巨型 DNA 和 F-肌动蛋白在拥挤环境中形成束

• 发表时间: 2008
• 作者: M. Negishi;T. Sakaue;K. Takiauchi;and K. Yoshikawa
• 通讯作者: and K. Yoshikawa

Nano Structures and Origanization of Compacted Single Chains of Polyelectrolytes

聚电解质致密单链的纳米结构和组织

• 发表时间: 2006
• 作者: A.A.Zinchenko;B.Damien;K.Yoshikawa (分担執筆)
• 通讯作者: K.Yoshikawa (分担執筆)

Modeling of living cell: Autonomous motion, micro-reactor and response to the environment

活细胞建模：自主运动、微反应器和对环境的响应

• 发表时间: 2007
• 作者: T.Fukuda;M.Nakajima;M.R.Ahmad;Y.Shen;M.Kojima;伊藤敏幸;K. Yoshikawa
• 通讯作者: K. Yoshikawa