Search for antitumor marine natural products through growth recovery assay in engineered yeasts

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Most important issue in the natural products discovery study is how to link the high-through-put in vitro assay results and cell-based in vivo activities. The growth recovery assay of the otherwise lethal gene-overexpressing mutant yeasts constructed by Dr. Yoshida is a method of choice for such purposes. From the extracts of marine invertebrates we isolated two truncated thiamine derivatives from the marine sponge Theonella swinhoei and three steroid glycosides from an unidentified marine sponge.