Metagenomic Analysis of Oral Microbiota from Normal Subjects and Patients with Periodontal Disease

正常受试者和牙周病患者口腔微生物群的宏基因组分析

• 批准号: 20249082
• 负责人: NOGUCHI Toshihide
• 金额: $ 30.95万
• 依托单位: Aichi Gakuin University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-20249082/
• 关键词: メタゲノム解析; 口腔細菌叢; 歯周病関連細菌; 歯周炎; ゲノムDNA抽出; 歯肉縁上プラーク; 歯肉縁下プラーク; 16SrRNA遺伝子解析

A metagenomic analysis of dental plaque microbiota obtained from periodontal healthy subjects and periodontitis patients was performed. The deep sequencing of the seven DNA samples detected total of 996 species and revealed substantially greater microbial diversity in the dental plaque than previously known. The functional binning profiles of these samples were compared with other environmental metagenome datasets such as human gut microbiota. Enrichments of genes in the categories of 'Defense mechanism' and 'carbon hydrate transport and metabolism' were observed in plaque samples.

对牙周健康受试者和牙周炎患者获得的牙菌斑菌群的宏基因组分析。七个DNA样品的深度测序检测到了总共996种，并且牙齿中的微生物多样性比以前所知要大得多。将这些样品的功能性归因曲线与其他环境元基因组数据集进行了比较。在斑块样品中观察到了“防御机理”和“水合碳输送和代谢”类别中基因的富集。

项目成果

メタゲノム解析技術の最前線

最前沿的宏基因组分析技术

• 发表时间: 2010
• 作者: Ryota Konda;Seiichi Ohta;Shigehiro Ishizuka;Joko Heriyanto;Agus Wicaksono.;伊藤英臣;伊藤英臣;伊藤英臣;妹尾啓史;伊藤英臣;伊藤英臣;伊藤英臣;伊藤英臣;服部正平
• 通讯作者: 服部正平

The effect of horizontal X-ray beam angulation on the detection of furcation defects of mandibular first molars in intraoral radiology.

口内放射学中水平X射线束角度对下颌第一磨牙分叉缺损检测的影响。

• 发表时间: 2010
• 期刊: Dentomaxillofac Radiol. 39
• 作者: Hishikawa T;Izumi M;Naitoh M;Furukawa M;Yoshinari N;Kawase H;Matsuoka M;Noguchi T;Ariji E.
• 通讯作者: Ariji E.

Degradation of vascular endothelial thrombomodulin by arginine-and lysin-specific cysteine proteases from Porphyromonas gingivalis

牙龈卟啉单胞菌精氨酸和赖氨酸特异性半胱氨酸蛋白酶降解血管内皮血栓调节蛋白

• 发表时间: 2009
• 期刊: J.Periodontol. 80
• 作者: Inomata M;Ishihara Y;Matsuyama T;Murayama I;Noguchi T;Matsushita K
• 通讯作者: Matsushita K

ヒト歯周病細菌叢のメタゲノム解析

人类牙周病细菌菌群的宏基因组分析

• 发表时间: 2011
• 作者: 林潤一郎,近藤伸二;亀井英彦;森田英利;足立直樹;福田光男;Todd D Taylor;石原裕一;小島俊男;野口俊英
• 通讯作者: 野口俊英

Metagenomic analysis of the dental plaque

牙菌斑的宏基因组分析

• 发表时间: 2010
• 作者: Hayashi J;Kondo S;Morita H;Kamei H;Fukuda M;Ishihara Y;Kojima T;Noguchi T
• 通讯作者: Noguchi T