The investigation of the role of cytokine from derived epithelial cell for activation γδ T cells in mucosal infection

为了解上皮内淋巴细胞在牙周病发病中的作用，本研究以C57 BL/6小鼠和BALB/c小鼠为研究对象，通过比较口腔感染单核细胞增生李斯特菌（Listeria monocytogenes）后小鼠肠上皮细胞（i-EC）早期IL-15的产生，探讨其在牙周病发病中的作用机制。活的L。单核细胞增多症，在第1、3或5天计数肝、脾、肠系膜淋巴结（MLN）和派尔集合淋巴结（PP）中的细菌。C57 BL/6小鼠的所有器官均表现出比BALB/c小鼠更高的抵抗力水平。要知道这些阻力的差异，我们调查的肠上皮内淋巴细胞（i-IEL）在口腔感染单核细胞增生李斯特菌的动力学。口腔感染后第1、3、5天，i-IEL中携带CD 8 αα和CDαβ或TCRαβ和γδ的亚群的绝对数量和比例均无显著性差异。因此，这些结果表明，口腔感染单核细胞增多性李斯特菌可能不会影响亚群， 关于我们 i-IEL的形成。接下来，我们研究了口腔感染后i-EC和i-IEL中细胞因子产生的差异。C57 BL/6小鼠i-EC感染单核细胞增生李斯特菌后第1天IL-15 mRNA和IL-15蛋白表达水平较高，而BALB/c小鼠i-EC中IL-15 mRNA和IL-15蛋白表达水平较低。同时，C57 BL/6小鼠i-IEL对CD 3刺激产生的IFN-γ量高于BALB/c小鼠。而BALB/c小鼠的i-IEL在CD 3刺激下产生的IL-4水平高于C57 BL/6小鼠。Toll样受体（TLR）2已被认为是来自分枝杆菌或革兰氏阳性细菌的细菌脂蛋白的受体。C57 BL/6小鼠感染单核细胞增生李斯特菌后第1天，i-EC中TLR 2 mRNA表达水平升高，提示TLR 2表达与IL-15产生的差异有关，并且i-EC早期产生IL-15的差异可能至少部分是C57 BL/6和BALB/c小鼠对单核细胞增生李斯特菌易感性差异的基础。少

To know the role of intraepithelial lymphocyte as an etiology of periodontal disease, the mechanism responsible for C57BL/6 as resistance mice and BALB/c as susceptibility mice to oral infection with Listeria monocytogenes were studied by comparing early IL-15 production in intestinal epithelial cells (i-EC).After mice were inoculated p.o. with viable L. monocytogenes, the bacteria in liver, spleen, mesenteric lymph nodes (MLN) and Peyer's patch (PP) were counted on day 1, 3 or 5. C57BL/6 mice were shown higher level of resistance than BALB/c in all organs. To know the difference of these resistance, we investigate kinetics of the intestinal intraepithelial lymphocytes (i-IEL) during oral infection with L.monocytogenes. There were no significant difference in the absolute : nmber and the ratio of i-IEL subpopulations bearing CD8αα and CDαβ or TCRαβ and γδ on day 1, 3 and 5 after oral infection. Thus, these results suggest that oral infection with L.monocytogenes may not affect the subp … More opulation of i-IEL in each mice. Next, we investigate the difference of cytokines production in i-EC and i-IEL after oral infection. High levels of IL-15 mRNA and IL-15 protein in i-EC from C57BL/6 mice were expressed on day 1 after L.monocytogenes infection, whereas in i-EC from BALB/c mice, those were expressed very low. Concurrently, a amount of IFN-γ which was produced by i-IEL from C57BL/6 mice in response to CD3 stimulation were higher than by that from BALB/c mice. While the i-IEL from BALB/c mice produced a higher level of IL-4 upon CD3 stimulation than that of C57BL/6 mice. The toll-like receptor (TLR) 2 has been suggested as a receptor for bacterial lipoproteins from Mycobacteria or Gram-positive bacteria. The mRNA expression level of TLR2, which is recognized to mediate a partly signaling for IL-15 gene transcription, was increased in i-EC from C57BL/6 mice on day 1 after L.monocytogenes infection.These results suggest that TLR2 expression regards difference of IL-15 production, and that the difference in early production of IL-15 by i-EC may at least partly underlie the difference in susceptibility to L.monocytogenes between C57BL/6 and BALB/c mice. Less