Basic Research for Aetiology and Pathology of Periodontal Disease

牙周病病因病理基础研究

基本信息

批准号：
61480396
负责人：
NOGUCHI Toshihide
金额：
$ 2.75万
依托单位：
Department of Periodontology,School of Dentistry,Aaichigakuin Universi (1987-1988)Tokyo Medical and Dental University (1986)
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1988
项目状态：
已结题

项目摘要

Based on the microbiological studies, it is well documented that the subgingival plaque, in which the gram-negative anaerobic rods are predominant, is associated with the destructive periodontal disease. In this investigation, we used hydroxypropylcellulose (HPC) as the base material and tried to apply the drugs directly to the subgingival plaque, the HPC containing 1% tetracycline (TC) formend solid and elastic strips. The in vitro release of the drugs from these strips showed almost 100% of this material added was released. From the clinical results obtained in this study, it was found that gingival index, probing depth and bleeding on probing tended to be less when the placebo strips were inserted. The results obtained from the microbiological studies showed that the TC containing HPC altered effectively the microbiological composition of the subginigival plaque.This new direct drug delivery system with HPC used as a base material was found to be effctive in changiang the composition of bacteria in the subgingival plaque and inproducing signs of clinical improvement.A note of caution should be added concerning the possible adverse in the subgingival flora which can be brought about by locally delivered tetracycline. Mandel et al. (1986) have shown that locally applied tetracycline in periodontal pockets infected with actinobacillus actinomycetemcomitans can actually result in a shift in the flora of such pockets to almost pure cultures of A. actinomycetemcomitans, associated with a period of active periodontal tissue destruction. It is likely that this results from re-seeding of the tetracycline-suppressed pocket by A.actinomycetemcomitans, which has invaded the gingival connective tissue and which remains viable in these tissue.

基于微生物学研究，有充分的文献证明，革兰氏阴性厌氧棒的尺寸斑块主要与破坏性的牙周疾病有关。在这项研究中，我们使用羟丙基纤维素（HPC）作为基本材料，并试图将药物直接应用于含1％四环素（TC）的HPC，形成固体和弹性条。从这些条带中的药物的体外释放显示，添加了几乎100％的这种材料。从这项研究中获得的临床结果中可以发现，插入安慰剂条时，牙龈指数，探测深度和探测探测的出血往往较小。从微生物学研究中获得的结果表明，含有HPC的TC有效改变了支柱斑块的微生物学组成。这种新的直接药物输送系统用HPC用作基础材料被发现是在樟宜的基础上效应的，在樟宜含量中，细菌的组成均可添加临床范围的范围。可以由本地交付的四环素带来的植物。 Mandel等。（1986年）表明，在感染了肌动杆菌静脉内菌的牙周口袋中局部应用的四环素实际上可以导致这种口袋的菌群转移到与活性牙周室内造成的几乎纯净植物菌的纯培养物中。这很可能是由于A.Actinomycetemcomitans对四环素抑制的口袋的重新种子而产生的，该口腔cotemcetemcomitans侵入了牙龈结缔组织，并且在这些组织中仍然可行。