Immunological properties of a heat schock protein from Actinobacillus actinomycetemcomitans.

来自放线杆菌伴放线杆菌的热休克蛋白的免疫学特性。

基本信息

批准号：
05454524
负责人：
NOGUCHI Toshihide
金额：
$ 3.97万
依托单位：
Department of Periodontology, School of Dentistry, Aichigakuin University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1995
项目状态：
已结题

项目摘要

Actinobacillus actinomycelemcomitans is a facultative gram-negative rod that is considered to be associated with human periodontal diseases. Heat shock proteins are a group of proteins with a conserved structure that are synthesized in response to a sudden increase in temperature or to other environmental stress. Among heat shock proteins, the 64-kDa GroEL-like protein has been implicated in the etiology of autoimmune diseases such as rheumatoid arthritis. In this study, we have examined biochemical and immunological properties of a GroEL-like protein from A.actinomycelemcomitans and other periodontopathogenic bacteria.SDS-polyacrylamide gel electrophoresis of SDS extracts from whole cells of A.actinomycelemcomitans Y4 showed that the shift from 30ﾟC to 42ﾟC or 50ﾟC brought about a significant increase in the relative amount of a 64-kDa protein. Monoclonal antibodies to the 64-kDa protein of A.actinomycelemcomitans cross-reacted with 62 to 64-kDa proteins of A.actinomycelemcomitans serotype a and c.Haemophilus aphrophilus, Haemophilus influenzae. Haemophilus paraphrophilus. and Escherichia coli. Rabbit antisera to whole cells of strain Y4 strongly reacted with the 64-kDa protein of A.actinomycelemcomitans Y4. Western blot analysis showed that alomost no sera from 18 healthy individuals reacted with the 64-kDa protein of A.actinomycelemcomitans Y4.although one serum from one healthy subject did react very weakly with the 64-kDa protein. Both sera from 2 localized juvenile periodontitis patients strongly reacted with the 64-kDa protein. In addition, 6 sera from 19 rapidly progressive periodontitis patients reacted with the 64-kDa protein. These results suggest that the 64-kDa protein of A.actinomycelemcomitans may be an immunodominant antigen of the organism.

放线杆菌放线菌Celemcitans是一种辅助革兰氏阴性杆，被认为与人类牙周疾病有关。热休克蛋白是一组具有组成结构的蛋白质，这些蛋白质是由于温度突然升高或其他环境应激而合成的。在热休克蛋白中，在自身免疫性疾病（如类风湿关节炎）的病因中已经浸渍了64 kDa凹槽样蛋白。在这项研究中，我们检查了来自腹膜杆菌和其他牙周病生成细菌的gro样蛋白的生物化学和免疫学特性。SDSDSDSD-LOLYACRYLAMIDE凝胶电泳提取物的凝胶凝胶电泳从整个细胞中的整个细胞中的细胞Y4的整个细胞Y4的整个细胞均从30 c或50 c或50 c或50 c cy c c c或50 c或50 c cy c c c或50 c c cy cor cor c或50 c或50 c或50 c c c c c cor cor cor cor cor cor cor cor cor cor cor cor cor a co cor cor的介于42 c c或50 c c c c cor cor。 64 kDa蛋白。与62至64 kDa蛋白交叉反应的64 kDa蛋白的单克隆抗体，肌动蛋白A. actinomycelemcitans a和haemophillus aphremophillus a actinomycelemcomitans a antymophilus a actinomycelemcomitans抗体。嗜血杆菌。和大肠杆菌。与Y4菌株的整个细胞的兔抗血清与A.Actinomycelemcomitans Y4的64 kDa蛋白强烈反应。 Western印迹分析表明，Alomost NO NO来自18个健康个体的血清与A.Actinomycelemcomitans的64 kDa蛋白反应。来自2名局部幼年牙周炎患者的两种血清都与64 kDA蛋白反应。此外，来自19名快速进行性牙周炎患者的6种血清与64 kDA蛋白反应。这些结果表明，肌动蛋白酶菌的64 kDa蛋白可能是该生物体的免疫抗原。