Immunological properties of a heat schock protein from Actinobacillus actinomycetemcomitans.

来自放线杆菌伴放线杆菌的热休克蛋白的免疫学特性。

• 批准号: 05454524
• 负责人: NOGUCHI Toshihide
• 金额: $ 3.97万
• 依托单位: Department of Periodontology, School of Dentistry, Aichigakuin University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05454524/
• 关键词: Periodontitis; Actinobacillus actinomycetemcomitans; Heat shock protein; GroEL-like protein; A. actinomycetemcomitans; 限局型若年性歯周炎; A.a.菌; 熱ショックタンパク質(HSP); GroEL様タンパク

Actinobacillus actinomycelemcomitans is a facultative gram-negative rod that is considered to be associated with human periodontal diseases. Heat shock proteins are a group of proteins with a conserved structure that are synthesized in response to a sudden increase in temperature or to other environmental stress. Among heat shock proteins, the 64-kDa GroEL-like protein has been implicated in the etiology of autoimmune diseases such as rheumatoid arthritis. In this study, we have examined biochemical and immunological properties of a GroEL-like protein from A.actinomycelemcomitans and other periodontopathogenic bacteria.SDS-polyacrylamide gel electrophoresis of SDS extracts from whole cells of A.actinomycelemcomitans Y4 showed that the shift from 30ﾟC to 42ﾟC or 50ﾟC brought about a significant increase in the relative amount of a 64-kDa protein. Monoclonal antibodies to the 64-kDa protein of A.actinomycelemcomitans cross-reacted with 62 to 64-kDa proteins of A.actinomycelemcomitans serotype a and c.Haemophilus aphrophilus, Haemophilus influenzae. Haemophilus paraphrophilus. and Escherichia coli. Rabbit antisera to whole cells of strain Y4 strongly reacted with the 64-kDa protein of A.actinomycelemcomitans Y4. Western blot analysis showed that alomost no sera from 18 healthy individuals reacted with the 64-kDa protein of A.actinomycelemcomitans Y4.although one serum from one healthy subject did react very weakly with the 64-kDa protein. Both sera from 2 localized juvenile periodontitis patients strongly reacted with the 64-kDa protein. In addition, 6 sera from 19 rapidly progressive periodontitis patients reacted with the 64-kDa protein. These results suggest that the 64-kDa protein of A.actinomycelemcomitans may be an immunodominant antigen of the organism.

伴放线放线杆菌是一种兼性革兰氏阴性杆菌，被认为与人类牙周疾病有关。热休克蛋白是一组具有保守结构的蛋白质，它们是响应温度突然升高或其他环境胁迫而合成的。在热休克蛋白中，64-kDa GroEL样蛋白与自身免疫性疾病如类风湿性关节炎的病因有关。本研究对伴放线放线菌和其他牙周致病菌的GroEL样蛋白进行了生物化学和免疫学特性的研究，对伴放线菌Y 4全细胞SDS提取物的SDS-聚丙烯酰胺凝胶电泳结果表明，从30 ℃到42 ℃或50 ℃的温度变化导致64-kDa蛋白的相对量显著增加。抗伴放线放线菌64 kDa蛋白的单克隆抗体与伴放线菌血清型a和嗜aphrophilus嗜血杆菌、流感嗜血杆菌的62至64 kDa蛋白发生交叉反应。嗜副沫嗜血杆菌。和大肠杆菌。抗Y 4菌株全细胞的兔抗血清与伴放线放线菌Y 4的64-kDa蛋白强烈反应。Westernblot分析表明，18名健康人血清几乎没有与伴放线放线菌Y 4的64-kDa蛋白反应，而1名健康人血清与64-kDa蛋白反应很弱。两个血清从2个本地化的青少年牙周炎患者强烈反应的64 kDa的蛋白质。此外，19例快速进展性牙周炎患者的6份血清与64 kDa蛋白反应。这些结果表明，放线菌的64-kDa蛋白可能是该生物体的免疫显性抗原。

项目成果

Keiko TAKADA,et al.: "Study of appiarence of serum antibody against GroEL-like protein and clinical data in periodontitis patients" J.Japan.Soc.Periodont. Vol.37. 114 (1995)

Keiko TAKADA 等人：“牙周炎患者血清抗 GroEL 样蛋白抗体的出现和临床数据的研究”J.Japan.Soc.Periodont。

高田敬子 他: "歯周病患者におけるGroEL様タンパク質に対する抗体の出現と臨床所見に関する研究-第二報-" 日本歯周病学会会誌. 37. 114- (1995)

Keiko Takada 等人：“针对牙周病患者的 GroEL 样蛋白抗体的出现和临床结果的研究 - 第二次报告 -”日本牙周病学会杂志 37. 114- (1995)。

野口俊英 他5名: "Role of prostaglandin in the formation of osteoclasts induced by capsular-like polysaccharide antigen of A. a. ateain Y_4" Oral Microbiol Immunol. 10. 69-75 (1995)

Toshihide Noguchi 等 5 人：“前列腺素在 A.a.atein Y_4 的荚膜样多糖抗原诱导的破骨细胞形成中的作用”口腔微生物免疫学。 10. 69-75 (1995)

T.Noguchi et.al: "The 64-Kilodalton Gro-EL-like protein of Actinobacillus actinomycetemcomitans" Journal of Periodontal Research. 28. 475-477 (1993)

T.Noguchi 等人：“Actinobacillus actinomycetemcomitans 的 64 千道尔顿 Gro-EL 样蛋白”《牙周研究杂志》。

T.Koga, et al.: "The 64-kilodalton GroEL-like protein of Actinobacillus actinomycetemcomitans" J.Periodont Res. Vol.28. 475-477 (1993)

T.Koga 等人：“Actinobacillus actinomycetemcomitans 的 64 千道尔顿 GroEL 样蛋白”J.Periodont Res。