Cell-surface components Actinobacillus actinomycetemcomitans promote osteoclast formation in vitro

细胞表面成分放线杆菌伴生促进破骨细胞形成体外

基本信息

批准号：
08457515
负责人：
NOGUCHI Toshihide
金额：
$ 4.54万
依托单位：
Aichi-gakuin University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1998
项目状态：
已结题

项目摘要

The mechanism of osteoclastic bone resorption induced by periodontopathic bacteria Actinobacillus actinomycetemcomitans cell-surface components was investigated in mouse organ cultures and in a mouse bone marrow culture system. Lipopolysaccharide and capsular polysaccharide isolated from A.actinomycetemcomitans Y 4 induced bone resorption in C 3 H/HeN mouse calvarium organ culture. Many multinucleated cells were formed when mouse bone marrow cells were cultured with A.actinomycetemcomitans Y 4 capsular polysaccharide or lipopolysaccharide for 9 days. The multinucleated cells showed several characteristics of osteoclasts, the production of including tartrateresistant acid phosphatase and the ability to resorb calcified dentine, in this study, monospecific antimouse recombinant interleukin-l alpha serum and indomethacin completely inhibited the formation of osteoclasts in the presence of A.actinomycetem comitans Y 4 capsular polysaccharide. An IL-1 receptor antagonist significantly inhibited the osteo clast formation in mouse marrow cultures. Bioactive IL-1 was detected in the culture supernatant of mouse bone marrow cells stimulated with A.actinomycetemcomitans Y 4 capsular polysaccharide. These results indicate that IL-1 alpha is involved in the mechanism of formation of osteoclast-like cells induced by A.actinomycetemcomitans Y 4 capsular polysaccharide. There was also a good correlation between the number of osteoclasts formed in the mouse marrow culture and the amount of prostaglandin E_2 released into the culture medium. These findings suggest that A.actinomycetemcomitans capsular polysaccharide may play an important role in inflammatory bone resorption by promoting osteoclast formation in periodontal diseases.

在小鼠器官培养物和小鼠骨髓培养系统中研究了由牙周病细菌放线症细菌静脉菌杆菌细胞静脉内切术诱导的破骨骨吸收的机制。脂多糖和囊囊多糖从A. actinomycetemcomitans y 4分离出的Y 4在C 3 H/HEN小鼠钙钙器官培养中诱导骨吸收。当小鼠骨髓细胞用肌分菌cotemcetans y 4囊多糖或脂多糖培养9天时，形成了许多多核细胞。 The multinucleated cells showed several characteristics of osteoclasts, the production of including tartrateresistant acid phosphatase and the ability to resorb calcified dentine, in this study, monospecific antimouse recombinant interleukin-l alpha serum and indomethacin completely inhibited the formation of osteoclasts in the presence of A.actinomycetem comitans Y 4 capsular多糖。 IL-1受体拮抗剂显着抑制了小鼠骨髓培养物中骨碎屑的形成。在用A. actinomycetemcitans Y 4囊多糖刺激的小鼠骨髓细胞的上清液中检测到生物活性IL-1。这些结果表明，IL-1α参与由肌分型菌comtemcomitans y 4囊多糖诱导的破骨细胞样细胞的机理。在小鼠骨髓培养物中形成的破骨细胞数量与将前列腺素E_2释放到培养基中的量之间也存在良好的相关性。这些发现表明，抗骨菌C囊囊多糖可能通过促进牙周疾病的破骨细胞形成而在炎症骨吸收中起重要作用。