Research for Clarifying Aetiologic Factors of Periodontal Disease -the Role of Neutrophil-

明确牙周病病因的研究-中性粒细胞的作用-

基本信息

批准号：
63870115
负责人：
NOGUCHI Toshihide
金额：
$ 1.6万
依托单位：
Aichigakuin University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research (B).
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1990
项目状态：
已结题

项目摘要

The purpose of the present study was to investigate the role of neutrophil on the development of periodontal disease. In animal study, the effect of a long-term methotrexate-induced neutropenia on the periodontal resion was histrogically and histometrically investigated.In the experimental side of the animals, a suturing nylon thread was inserted into the interdental space between the left maxillary 1 st and 2 nd molars.The right side of the animals of the same area was used as control side without doing any treatments. Then, the animals were divided equally into two groups.The animals in one group were injected with 1.0mg/kg of methotrexate (MTX), whereas in the another group with no injection of M T X. Results obtained were as follows.The numbers of white blood cell and neutrophil in the M T X injected group decreased remarkably from 3 to 9 weeks. The experimental side of non M T X injected group showed a slight gingival inflammation and moderate alveolar bone resorption at 7 and 9 weeks. In the experimental side of M T X- injected group, severe alveolar bone resorptions were found at 7 and 9 weeks.The difference of bone resorption between these two groups was statistically significant. The results of the present study indicated that long-term M T X induced neutropenia changed the moderate periodontitis into the severe one. From the study of congenital neutropenia in human beings, it was also suggested that neutrophil play a important role for developing of periodontal disease.

本研究的目的是研究中性粒细胞在牙周疾病发展中的作用。在动物研究中，在动物的实验侧进行了雌性和组织学研究的长期甲氨蝶呤诱导的中性粒细胞减少对牙周回旋的影响。在动物的实验侧，将缝合的尼龙线插入了左侧颌骨1 st和2 nd molar之间的互化空间，而没有任何用作动物的右侧。然后，将动物平均分为两组。将一组的动物注射1.0mg/kg甲氨蝶呤（MTX），而在另一组中未注入M T X的动物。所获得的结果如下。在3周至9周中，M t X注入的白细胞和中性粒细胞的数量降低了。非M t X注射组的实验侧显示，在7周和9周时显示出轻微的牙龈炎症和中等的肺泡骨吸收。在M t X X注射组的实验侧，发现在7周和9周时发现严重的肺泡骨解测。这两组之间的骨吸收差具有统计学意义。本研究的结果表明，长期M T X诱导的中性粒细胞减少症将中度牙周炎变成了严重的牙周炎。从人类先天性中性粒细胞减少症的研究中，还提出中性粒细胞在发育牙周疾病中起重要作用。