Methodological studies for the control of three invasive alien ant species using molecular-biological techniques, sterile males and natural enemies

我们证实，阿根廷蚂蚁正在扩大它们在日本太平洋一侧沿海地区的分布，尽管我们成功地使用Z-(9)-十六烯醛和一种常见的杀菌剂根除了横滨的一个小种群。为了防止RIFA入侵日本，我们开发了识别(使用DNA条形码)和检测(使用寄生蜂Micropltis croceipe)的方法，即使是检疫人员也可以很容易地使用。在台湾、美国和阿根廷的调查中，我们发现在RIRA最近入侵的国家，多倍体工作者迅速增加。利用Diacamma sp.我们研究了蚂蚁的性别决定机制，发现了一些有趣的基因表达模式，如女性化基因。

We confirmed that Argentine ants are expanding their distribution along the coastal areas of Pacific Ocean side in Japan, though we succeeded in eradication of one small population in Yokohama using Z-(9)-Hexadecenal as well as a common myrmecocide. To prevent the invasion of RIFA into Japan, we developed methods for identification (using DNA bar coding) and detection (using the parasitoid wasp Microplitis croceipes) which can readily be used even by quarantine officers. In the surveys in Taiwan, USA and Argentina, we found that polyploid workers rapidly increased in the countries where RIRA had recently invaded. Using Diacamma sp. which is useful for molecular biology, we studied the sex determination mechanism in ants and found some intriguing expression patterns of genes such as feminizer.