Biophysical characterization of protein interactions within a transcription factor network

基因表达在一定程度上是由称为转录因子和共调节因子的蛋白质对和蛋白质组之间的相互作用来调节的。这些蛋白质在基因启动子和增强子处组装成复合体，从而控制该基因的表达。在分子水平上，这些络合物是如何形成的，不同的相互作用是如何相互合作或竞争的，人们知之甚少。在这项提案中，我们的目标是定义参与心脏发育的两个转录调控因子(HOP和SRF)之间的复合体，并开始定义组成正常心脏发育所必需的转录网络的其他相互作用。

Gene expression is regulated in part by interactions between pairs and groups of proteins known as transcription factors and co-regulators. These proteins assemble into complexes at gene promoters and enhancers and thereby control the expression of that gene. Little is known at the molecular level of how these complexes form and how different interactions cooperate or compete with each other. In this proposal we aim to define a complex between two transcriptional regulators (HOP and SRF) involved in cardiac development and to begin to define other interactions that make up a transcriptional network essential for development of a normal heart.