The effect of nitrogen monoxide on intracellular iron metabolism

一氧化氮对细胞内铁代谢的影响

• 批准号: DP0450213
• 负责人: Prof Des Richardson
• 金额: $ 17.76万
• 依托单位: The University of Sydney
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2004
• 资助国家: 澳大利亚
• 起止时间: 2004-04-15 至 2007-06-30
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP0450213
• 关键词: effect; nitrogen; monoxide; intracellular; iron

During our current ARC grant we discovered a novel relationship between energy metabolism and NO-mediated Fe efflux and showed that glutathione (GSH) is vital for this release mechanism (DR5,6). Intriguingly, this transport process is part of the cytotoxic effector machinery of activated macrophages against tumours, and requires further elucidation. We also showed that CO affects Fe metabolism by binding to Fe, and CO may modulate NO's function. We will:- (1) Examine if NO-mediated Fe release results in GSH efflux (2) Identify the mechanism of NO-mediated Fe efflux. (3) Assess the effect of inducing haem oxygenase 1 on Fe metabolism

在我们目前的ARC资助期间，我们发现了能量代谢和NO介导的铁外流之间的新关系，并表明谷胱甘肽(GSH)在这种释放机制中至关重要(DR5，6)。有趣的是，这一转运过程是激活的巨噬细胞抗肿瘤的细胞毒效应机制的一部分，需要进一步阐明。我们还表明，CO通过与Fe结合来影响Fe代谢，CO可能调节NO的功能。我们会： (1)检查NO介导的铁释放是否导致GSH外流 (2)明确NO介导的铁外流机制。 (3)评价诱导血红素加氧酶1对铁代谢的影响