Studies of biological function and relationship between structure and function of major-type sigma factors

1.使用rpoS-deficient （rpoS：（Tn 10） strains，一个依赖于rpos的katE启动子被其他人以前报道过的区域不同。在更远的地方，在促进者区域找到了一个-10个一致的六聚氰胺序列，但是？35个序列不是。A carboxy-terminal region responsible for the？D138？D1-speific salt-Tolerance was由体外转录实验和命名CTE （carboxy-terminal element）标识。2.第2组Cyanobacterium Synechococcus sp.JPCC7942显示要识别E.大肠杆菌的规范性启动子，如体外实验中的普通启动子。一种蛋白质分析，Synechocystis sp.PCC6803已经完成，并建议在特定基因在营养不良生长条件下的定义中纳入sigD和sigE基因。3.多核基因（sig）编码塑料RNA聚合酶sigma因子已从红藻、Cyanidium Caladium RK-1和更高的植物中识别出来，包括阿拉伯毒酵母、烟草和大米。对这些基因的结构性和功能性分析是如何被带走的。过度表达和纯化的基因产品在体外表现出西格玛活动的迹象。给A.。Thaliana，三种新的sig基因，分别命名为sigD、sigE和sigE，以及sigA、sigB和sigC，这些基因被确定为已确定的和染色体位置。Furthermore，使用uid和GFP基因融合和转化将花生植物、光诱导性、基因产品的本地化以及这些基因在塑料发育期间的空间和临时表达进行了分析。

1. Using rpoS-deficient (rpoS:: Tn10) strains, an rpoS-dependent katE promoter was delimited in a region different from that previously reported by others. Furthermore, in the promoter region a -10 consensus hexamer sequence was found, but ?35 sequence was not. A carboxy-terminal region responsible for the σ ィイD138ィエD1-speeific salt-tolerance was identified by in vitro transcription experiments and named CTE (carboxy-terminal element). 2. Group 2 sigma factors of cyanobacterium Synechococcus sp. JPCC7942 were shown to recognize E. coli canonical promoters as well as cyanobacterial common promoters by in vitro, experiments. A proteome analysis, of Synechocystis sp. PCC6803 was performed and it was suggested that sigD and sigE genes were involved in the induction of specific genes during nitrogen-deficient growth condition. 3. Multiple nuclear genes (sig) encoding plastid RNA polymerase sigma factors were identified from red algae, Cyanidium caladium RK-1 and higher plants including Arabidopsis thaliana, tobacco and rice. Structural and functional analyses of these genes were carried out. The overexpressed and purified gene products were shown to have sigma activity in vitro. For A. thaliana, three new sig genes, named sigD, sigE, and sigE, besides sigA, sigB and sigC, were identified and chromosomal locations of these genes were determined. Furthermore, using uid and GFP gene fusion and transformation into Arabidopsis plants, light inducibility, localization of gene products and spacial and temporal expression of these genes during plastid development were analyzed.