Effect of intense sports activity on carnitine metabolism in youth and children

剧烈体育活动对青少年和儿童肉毒碱代谢的影响

• 批准号: 09670804
• 负责人: INOUE Fumio
• 金额: $ 2.24万
• 依托单位: KYOTO UNIVERSITY OF EDUCATION
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670804/
• 关键词: SPORTS; CARNITINE; BUTYROBETAINE; TRIMETHYLLYSINE; PIVALIC ACID; カルニテン

Background : Carnitine plays an important role in fatty acid oxidation, that is one of main energy sourses of muscles during aerobic exercise. Recently, several drugs (some of antibiotics, anticonvulsants, antineoplastics) have been reported to cause carnitine deficiency. Children and youth, who participate in intense regular exercise, may have an opportunity to take these drugs.Objective : To study the effects of intense physical activity on carnitine metabolism in youth and children.Methods : Carnitine and its precursors were analyzes by tandem mass spectrometry.Results : Analytical methods for free carnitne, acylcarnitines, butyrobetaine, trimethyllysine were established by tandem mass spectrometry in secondary ion mass spectrometric mode, that enabled us to conduct the study of physical exercise because of a small amount of sample volume required. Sports activity caused increased serum levels of butyrobetaine, acetylcarnitine, C4-and C8-acylcarnitines and decreased serum levels of free carnitine. Administration of pivalate-containing antibiotic caused decreased serum levels of free carnitne and increased serum CK and myoglobin.Conclusion : Carnitine deficiency induced by drug administration may cause impaired fatty acid oxidation and metabolic damage of muscular tissue.

研究背景：肉毒碱在有氧运动中作为肌肉的主要能量来源之一的脂肪酸氧化中起重要作用。最近，几种药物（一些抗生素，抗惊厥药，抗真菌塑料）已被报道会导致肉毒碱缺乏症。儿童和青少年，谁参加激烈的定期运动，可能有机会采取这些药物。目的：研究激烈的体育活动对青年和儿童的肉毒碱代谢的影响。方法：肉毒碱及其前体物的串联质谱分析。结果：游离肉毒碱、酰基肉毒碱、丁酰甜菜碱、三甲基赖氨酸的二次离子质谱模式的串联质谱建立，使我们能够进行体育锻炼的研究，因为少量的样品体积所需。运动活动引起血清水平的丁酰甜菜碱，乙酰肉毒碱，C4-和C8-酰基肉毒碱和降低血清水平的游离肉毒碱。结论：肉毒碱缺乏可导致脂肪酸氧化障碍和肌肉组织代谢损伤。

项目成果

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Inoue F et al.: "Effect of sports activity carnitine metabolism -Measurement of free carnitine,γ- butyrobetaine and acylcarnitines by tandem mass spectrometry-"Journal of Cromatography B. 731. 83-88 (1999)

Inoue F等：“运动活动肉碱代谢的影响-通过串联质谱法测定游离肉碱、γ-丁甜菜碱和酰基肉碱-”色谱杂志B.731.83-88(1999)

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Fumio Inoue：“体育活动对肉碱代谢的影响”日本医学质谱学会会刊 23. 169-172 (1998)。