IMAGING OF AVASCULAR NECROSIS OF FEMORAL HEAD IN SPONTANEOUS HYPERTENSIVE RATS AND ITS EXPERIMENTAL MODEL

自发性高血压大鼠股骨头血管坏死的影像学及其实验模型

• 批准号: 09670927
• 负责人: TAKADA Masahiko
• 金额: $ 1.86万
• 依托单位: SHIGA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670927/
• 关键词: micro CT; Trabecular structure; multi-detector CT; osteoporosis; 易脳卒中自然発生高血圧ラット; 大腿骨頭壊死; 核磁気共鳴装置; マイクロC.T; グレディエントエコー法; 自然発生高血圧ラット; 核磁気共鳴

We originally aimed to obtain magnetic resonance images of avascular necrosis of femoral head in spontaneous hypertensive rats, however, we failed to image them due to too small size of them such as 2mm in a diameter. Therefore, we change our plan and tried to obtain 3 dimensional analysis of trabecular structure of the femoral heads in spontaneous hypertensive rats using micro computed tomography. We obtained some results for the trabecular structural analysis, but, unfortunately, they were not promising of further study. We, again, changed our plan and tried to perform in vivo three-dimensional analysis of trabecular structure of human vertebra using data from multi-detector CT. We successfully read image data of multi-detector CT using the software for three-dimensional analysis of trabecular structure built in the micro CT. We performed precision study for the three-dimensional analysis using vertebra of a human cadaver. We evaluated three dimensional parameters of bone volume (BV) , bone surface (BS), bone density (BV/TV), trabecular thickness (Tb. Th), trabecular space (Tb. Sp), trabecular bone factor (TBPf) , structure model index (SMI) , number of nodes (N. Nd). We measured the specimens five times and calculated coefficient variation (CV). The CVs in BV, BS, BV/TV, Tb. Th, Tb. Sp, TBPf, SMI, N. Nd were 1.8 %, 0.91 %, 1.8 %, 1.4 %, 1.5 %, 7.7 %, 0.81 %, 2.5 %, respectively. The results were comparable to the precision of bone densitometry by dual x-ray absorptiometry. This suggest that in vivo three dimensional analysis of trabecular bone structure would be used clinically for evaluating effects of drugs for osteoporosis on trabecular bone structure.

我们最初的目的是获得自发性高血压大鼠股骨头缺血性坏死的磁共振图像，然而，我们未能成像，由于太小的尺寸，如直径为2 mm。因此，我们改变了我们的计划，并试图获得三维分析的骨小梁结构的自发性高血压大鼠股骨头的微型计算机断层扫描。我们获得了一些结果的小梁结构分析，但不幸的是，他们没有希望进一步研究。我们再次改变了计划，尝试使用多探测器CT的数据对人体椎骨的小梁结构进行体内三维分析。我们成功地读取图像数据的多排CT使用软件的三维分析小梁结构内置在微型CT。我们使用人体尸体的椎骨进行三维分析的精度研究。测量骨体积（BV）、骨表面（BS）、骨密度（BV/TV）、骨小梁厚度（Tb. Th）、小梁间隙（Tb. Sp）、骨小梁因子（TBPf）、结构模型指数（SMI）、节点数（N. Nd）。我们测量了标本五次，并计算了变异系数（CV）。BV、BS、BV/TV、Tb. Th，Tb。Sp、TBPf、SMI、N. Nd分别为1.8%、0.91%、1.8%、1.4%、1.5%、7.7%、0.81%、2.5%。结果与双能X线吸收法骨密度测定的精密度相当。提示骨小梁三维结构分析可用于临床评价治疗骨质疏松药物对骨小梁结构的影响。