Visualization of cortico-basal ganglia loop by means of transsynaptic transport of WGA-recombinant adeno virus and rabies virus

通过 WGA 重组腺病毒和狂犬病病毒的突触运输实现皮质基底节环的可视化

• 批准号: 13680833
• 负责人: TAKADA Masahiko
• 金额: $ 2.05万
• 依托单位: Tokyo Metropolitan Organization for Medical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13680833/
• 关键词: Cerebral Cortex; Basal Ganglia; Cerebellum; Motor Control; Adeno Virus; Rabies Virus; Transsynaptic Transport; Monkey; 線条体; 淡蒼球; 黒質; 運動領野; 前頭連合野

Retrograde transneuronal labeling by means of transsynaptic transport of WGA-recombinant adeno virus and rabies virus was attempted to analyze the organization of functionally-related multi-synaptic pathways, for example, the cortico-basal ganglia loops that arise from various frontal motor and association areas. In this report, we have only described the data obtained from a series of experiments using rabies virus, because rabies virus was found to be much more sensitive than WGA-recombinant adeno virus for the present purpose. Injections of rabies virus (CVS-11; CDC) were made into the primary motor cortex (MI) to investigate the extent of its retrograde transport in the central motor system. A viral suspension was injected into the electrophysiologically identified forelimb region of the MI in rhesus monkeys. The monkeys underwent perfusion-fixation two to four days after the injection. Serial coronal sections were processed for immunohistochemical staining for rabies virus. Three … More days after viral injection, many second-order neurons were labeled, along with the first-order neurons in the thalamus and the cerebral cortex that project to the MI directly. In the basal ganglia, the labeled neurons were observed in the ventral aspect of the internal segment of the globus pallidus. Moreover, neuronal labeling occurred in the deep cerebellar nuclei, especially in the dorsal portions of the lateral and interpositus nuclei, and in several rostrally-situated motor-related areas of the frontal lobe. Around three and a half days after the injection, third-order neurons were seen in the ventral aspects of the putamen, the subthalamic nucleus, and the external segment of the globus pallidus, as well as in Purkinje cells of the cerebellar cortex and areas of the prefrontal cortex. In addition, our immunofluorescence analysis confirmed that cholinergic striatal interneurons and dopaminergic nigral neurons, probably fourth-order neurons in the basal ganglia, were labeled at the four-day postinjection period. Less

通过WGA重组腺病毒和狂犬病病毒的跨突触转运的逆行跨神经元标记试图分析功能相关的多突触通路的组织，例如，从各种额叶运动和关联区域产生的皮质基底神经节环。在本报告中，我们仅描述了从使用狂犬病病毒的一系列实验中获得的数据，因为发现狂犬病病毒比WGA重组腺病毒对本目的更敏感。将狂犬病毒（CVS-11; CDC）注射到初级运动皮质（MI）中，以研究其在中央运动系统中逆行转运的程度。将病毒悬液注射到恒河猴MI的电生理学鉴定的前肢区域中。在注射后2至4天，对猴子进行灌注固定。连续冠状切片进行狂犬病毒免疫组化染色。三 ...更多信息 病毒注射后30天，许多二级神经元被标记，沿着的还有丘脑和大脑皮层中直接投射到MI的一级神经元。在基底节，标记神经元位于苍白球内段的腹侧。此外，神经元标记发生在小脑深核，特别是在背侧部分的外侧核和间位核，并在几个rostrally-located电机相关领域的额叶。注射后约三天半，在壳核腹侧、丘脑底核和苍白球外段，以及小脑皮质的浦肯野细胞和前额叶皮质区域中观察到三级神经元。此外，我们的免疫荧光分析证实，胆碱能纹状体中间神经元和多巴胺能黑质神经元，可能是基底神经节的第四阶神经元，在注射后第四天被标记。少

项目成果

Kitano K, Cateau H, Kaneda K, Nambu A, Takada M, Fukai T: "Two-sate membrane potential transitions of striatal spiny neurons as evidenced by numerical simulations and electrophysiological recordings in awake monkeys"J Neurosci. 22 RC230. 1-6 (2002)

Kitano K、Cateau H、Kaneda K、Nambu A、Takada M、Fukai T：“清醒猴子的数值模拟和电生理记录证明了纹状体多棘神经元的双态膜电位转换”J Neurosci。

Kitano K, Cateau H, Kaneda K, NambuA, Takada M, Fukai T: "Two-state membrane potential transitions of striatal spiny neurons as evidenced by numerical simulations and electrophysiological recordings in awake monkeys"J Neurosci. 22:RC230. 1-6 (2002)

Kitano K、Cateau H、Kaneda K、NambuA、Takada M、Fukai T：“清醒猴子的数值模拟和电生理记录证明了纹状体多棘神经元的两种状态膜电位转换”J Neurosci。

Kaneda K, Nambu A, Tokuno H, Takada M: "Differential processing patterns of motor information via striatopallidal and striatonigral projections."J Neurophysiol. 88. 1420-1432 (2002)

Kaneda K、Nambu A、Tokuno H、Takada M：“通过纹状体苍白球和纹状体黑质投影的运动信息的差异处理模式。”J Neurophysicalol。

Takada M, Matsumura M, Kojima H, Yamaji Y, Inase M, Tokuno H, Nambu: "Protection against nigrostriatal dopamine cell death by pedunculopontis tegmental nucleus lesions"The Basal Ganglia VII. 67-76 (2002)

Takada M、Matsumura M、Kojima H、Yamaji Y、Inase M、Tokuno H、Nambu：“脚桥被盖核损伤对黑质纹状体多巴胺细胞死亡的保护”基底神经节 VII。

Takada M: "Immunohistochemical locallzation of voltage-gated calcium channels in substantia nigra dopamine neurons"Eur J Neurosci. 13. 757-762 (2001)

Takada M：“黑质多巴胺神经元中电压门控钙通道的免疫组织化学定位”Eur J Neurosci。