A Cytobiochemical Characterization of Cell Diferentiation Derived from Calcified and Non-calcified Tissues.

从非钙化组织来源的牙周韧带细胞(PLF)和成纤维细胞的特征细胞功能角度探讨了钙化的机制，并与过去积累的大鼠颅骨成骨细胞进行了比较。牙周膜主要是含有各种非胶原性成分的纤维组织，将牙齿与牙槽骨紧密联系在一起。虽然牙周膜对肝脏的摄氧量为肝脏的1/50(&lt；Q_O2&gt；/DNA=0.2)，但通过在体外短期培养体系中加入琥珀酸-1，4-&lt；(14)C&gt；的中间产物的转氨化和糖异生作用，发现氨基酸、蛋白质和糖胺聚糖合成的代谢活性广泛。牛PLF的碱性磷酸酶(ALPase)活性明显高于皮肤成纤维细胞，皮肤成纤维细胞的表型似乎与大鼠成骨样细胞相似，但并不完全相同。人PLF和牙槽骨制备的ALPase具有与牛PLF相同的酶学性质。现在可以将PLF称为成骨成纤维细胞，并将其作为工作假说提出。本研究的重要发现是1，25-lt；(OH)_2&gt；&lt；D_3&gt；和地塞米松对PLF和骨细胞的ALPase活性有调节作用，并发现在生理浓度范围内，66Kd的磷酸化蛋白作为活性&lt；D_3&gt；的剂量反应蛋白，可能对磷酸八钙的转化起重要作用。此外，通过与NS-S肌瘤的细胞融合，获得了3株抗人PLF的单抗合成细胞株。上述结果为研究牙周膜钙化过程提供了新的方面，主要包括抑制牙周膜矿化的机制。

The mechanism of calcification was investigated in terms of characteristic cell function of periodontal ligament cell (PLF) and fibroblast derived from non-calcified tissues in comparison with osteogenic cells from rat pup calvaria and bone in which extensive researches had been accumulated during past decays.The periodontal ligament is mainly of fibrous tissue containing various non-collagenous components, which tightly connects the tooth to the alveolar bone. Although the oxygen uptake of periodontal ligament was measured as 1/50 to that liver ( <Q_O2> /DNA=0.2), the metabolic activities of amino acids, proteins and glycosaminoglycans synthesis were found extensive through transamination and gluconeogenesis of intermediates converted from succinate-1,4- <^(14)C> which was added in the medium of short-term incubation system in vitro. Bovine PLF has a remarkably high activity of alkaline phosphatase (ALPase) compared to skin fibroblasts, which seem to have similar, but not identical phenotype with that of the rat osteoblast-like cells. The ALPase prepared from the human PLF and alveolar bone exhibited the same enzymatic character with bovine PLF. It is now possible to refer to PLF as an osteoblastic fibroblast and has proposed this as a work hypothesis. Important findings of this research are that ALPase activity of PLF and bone cells was regulated by 1,25 <(OH)_2> <D_3> and dexamethasone, and also 66Kd phosphoprotein was found as dose responding protein of active <D_3> within physiological concentration, which may have an important role for the transformation of octacalcium phosphate. Furtheremore, three stains of monoclonal antibodies synthesized cell line for human PLF were obtained by the cell fusion using myenoma NS-S.Those results mentioned above provide new aspect for the processes of calcification which mainly consists of inhibitory mechanism of mineralization observed in periodontal ligament.