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癌の染色体異常の発生とその意義に関する分子細胞遺伝学的研究

癌症染色体异常发生及意义的分子细胞遗传学研究

基本信息

批准号：
60010053
负责人：
杉山武敏
金额：
$ 7.68万
依托单位：
Kobe University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Cancer Research
财政年份：
1985
资助国家：
日本
起止时间：
1985 至无数据
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60010053/
关键词：
癌染色体分子遺伝学癌遺伝子遺伝子マッピング染色体異常

项目摘要

共通目標、癌化に関する遺伝子の分子細胞遺伝学的マッピングと、情報・技術の交換に班員の努力目的が固まってきた。癌の染色体変化と癌遺伝子の組換え、発現を調べる研究に就いて、班内外で多角的な研究効力のかたちがとられつつある。一方で班員独自の研究が進行した。高木班員はCattanach転座でのX下活性を指標に癌細胞が単一細胞、あるいは多細胞起源かを探った。及川班員のラットでの癌遺伝子myc,Nras,Hras,fosの染色体、を決定した。S197形質細胞のmyc発現は脾細胞との融合では抑制されず繊維芽細胞とでは抑制され、制御遺伝子の組織依存性を示した。金子班員によれば各種【Ph^1】白血病でbcr変異、ll923転座でのets1の組換えはなかった。大野班員は鉱物油によるマウス形質細胞腫の異型転座でmycが15D2から12F1に転座していた。沼班員のenkephalin遺伝子のマッピングは、異種cDNAでは雑種効率悪く、ヒトcDNAより反復配列のない部分がsubc1oneされ、可能性が出てきた。翠川班員は昨年度マウス無血清培地増殖株(m細胞)で増殖性と染色体変化との関連を解析した。阿部班員は多数の培養株の核型を明らかにし、癌遺伝子研究の基礎を作った。また神経芽細胞株で、ビオチン化DNAプローブを用いる癌遺伝子のマッピングを検討するかたわら、po1y-LYSを用いるNmycのマッピングを進めている。杉山班員はラット白血病について、ab1-rDNA転座の分子転座の証明を試みているが、ab1の5′,3′側に20kbまでrDNAの遺伝子は確認出来ず、3′側にrDNAのスペーサー要素を認め、意味を検討中である。白石班員はB100m患者(BS)リンパ球の発癌剤変換細胞の染色体変化と癌遺伝子発現、骨髄細胞系への分化を認めた。BS細胞のSCE発生機構も継続検討中である。

The common goal, the molecular biology of cancer, the communication of technology and technology, and the efforts of the members of the class are aimed at solidifying the disease. The chromosomal transformation of cancer cells, the histology of cancer cells, the study of cancer cells, and the multi-angle research in and out of the class. One of the team members studied and carried out the study alone. High wood class member Cattanach seat "active" refers to cancer cell "one cell", "multiple" cell origin "exploration". And members of the Sichuan team checked the chromosome and determination of myc,Nras,Hras,fos in the cancer cell. S197 cell myc showed that the spleen cells were fused, the cells were inhibited, the germ cells were suppressed, and the tissue dependence was detected. Members of the gold team conducted a variety of [Ph ^ 1] leukemia bcr tests and ll923 seat ets1 tests. Members of the Ono crew have a myc 15D2 seat, a 12F1 seat, a cell phone, a cell phone, a cell phone. The biogas crew members of the enkephalin team will be responsible for the production of several kinds of cDNA equipment, and the frequency of each kind of subc1one will be increased. The subc1one will be allocated to the staff of the biogas crew in the opposite direction. Last year, the staff of Cuichuan did not have blood serum to culture the reproductive plants (m cells). The chromosomes of reproductive chromosomes were analyzed and analyzed. Most of the members of the Abe team identified the karyotype of the strains and the basis for the study of cancer. The cell line was isolated from the sprouts, the DNA was transformed into the cell line, and the cancer cell line was treated with the cancer cell line. The cancer cell line was treated with the cancer cell line, and the cell line was treated with the Nmyc cell line, and the po1y-LYS was modified with the Nmyc cell line. Members of the Sugiyama team checked the leukemias, the molecular blocks of the ab1-rDNA, the ab1, the ab1, the 20kb, the rDNA, the confirmed, the rDNA, the elements, which meant that they were in trouble. In patients with B100m in Baishi class (BS), the chromosomes of cancer cells were detected, and the differentiation of bone cell lines was confirmed. The BS