Replication and division of the structure in plant cells

在不同的植物细胞中，对细胞核、线粒体、腺体、鞭毛等细胞器的复制和分裂以及细胞壁微管的排列进行了合作研究。1)用电子显微镜观察了野生型和腺苷环化酶缺陷型酿酒酵母突变株SPB的复制，并用冷冻替代法观察到一种特殊的S型纺锤体。2)研究了发芽酵母和绒泡菌原生质体中线粒体类核的融合和分裂，制备了与线粒体DNA结合的膜组分。3)细胞松弛素B和免疫印迹显示，蓝藻叶绿体分割面周围的环状结构由肌动蛋白细丝组成。研究了不同生理条件下纤细裸藻细胞周期中细胞核与叶绿体的紧密联系。4)用电子显微镜观察了艾氏新月浑藻受精卵萌发过程中的发育和分裂，以及尖生栅藻中的二联体的结构和功能。5)用免疫荧光显微镜观察了蜘蛛和腹主动脉壁微管的排列。6)研究了植物激素(生长素、赤霉素、细胞分裂素)对百日草叶肉细胞皮质微管排列和DNA合成的影响。通过偏振光照射和细胞离心法，分析了蓝光诱导蕨类原生线虫细胞分裂的光反应部位。7)研究了Prasinophyceae鞭毛器的复制与有丝分裂纺锤体发育的关系。

Researches on the replication and division of cellular organelles such as nuclei, mitochondria, dictyosomes, flagella and the cell wall microtubule arrangements were cooperatively performed in a variety of plant cells. 1) Duplication of SPB of both wild and adenylate cyclase-deficient mutant of the yeast Saccharomyces cerevisiae was studied by electron microscopy, and a peculiar sigmoidal spindle was also found by freeze substitution method. 2) Fusion and division of the mitochondrial nucleoid were studied in the budding yeast and in plasmodia of Physarum, and a membrane fraction binding the mitochondrial DNA was prepared. 3) Effects of cytochalasin B and western blotting showed that a ring structure around the dividing plane of Cyanidium caldarium chloroplast consisted of actin filaments. Close association between the nucleus and chloroplasts in the cell cycle of Euglena gracilis was studied under different condition physiological conditions. 4) Growth and division of the dictyosomes during zygote germination of Closterium ehrenbergii and the structure and function of dictyosomes in Scenedesmus acuminatus were studied by electron microscopy. 5) The arrangements of wall microtubules in Spirogyra and in Vinca major were studied by immunofluorescent microscopy. 6) Effects of plant hormones (auxin, gibberellin, cytokinin) were studied in relation to the arrangement of cortical microtubules and DNA synthesis in isolated Zinnia mesophyll cells. Intracellular photoreactive site for blue light-induced cell division in protonemata of the fern was analyzed by polarized irradiation and cell centrifugation. 7) Replication of the flagellar apparatus in the alga Prasinophyceae was studied in relation to the development of mitotic spindle.