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Effect of physical exercise on the permeability of the cellular membrane to enzymes and its mechanism

体育锻炼对细胞膜对酶通透性的影响及其机制

基本信息

批准号：
61570082
负责人：
OHNO Hideki
金额：
$ 1.34万
依托单位：
Asahikawa Medical College
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1987
项目状态：
已结题

项目摘要

The purpose of the present study was to investigate the influence of physical exercise on the permeability of the cellular membrane to enzymes. The following three experiments were conducted: 1. Running training: the effects of 10-week physical training (running over 5 km, 6 times/week) on both the activity and concentration of <gamma>-glutamyl transpeptidase (<gamma>GTP) in plasma were investigated on seven sedentary male students. After training the resting plasma <gamma>GTP concentration decreased to half, whereas the <gamma>GTP activity did not vary substantially. This suggests that after training the precursor form of <gamma>GTP did not appear in the plasma and that 'activator protein' appeared in the plasma, possibly due to a leakage from the tissues such as liver. 2. Prolonged exercise: Fifteen male Wistar rats were exercised by swimming until exhausted with a weight equal to 3 % of their body weight in water at 35゜C. Microsomal <gamma>GTP was studied in the liver cell fractions. In vivo and in vitro permeabilities for both <gamma>GTP activity and content were calculated by the following formulas:In vivo permeability (%)= (extramicrosomal fraction x 100 )/(tramicrosomal fraction + microsomal fraction ) andin vitro permeability (%) = ( hyposmotic condition )/(osmotic condition ) x 100The in vivo permeability of both <gamma>GTP activity and content was greatly enhanced after swimming, whereas the in vitro permeability did not change significantly. This indicates that acute exercise increases the permeability of the microsomal membrane of rat liver. 3. Swimming training: Ten male Wister rats were exercised by swimming to 3 h/day, five times/week for 10 weeks in water at 35゜C. After training both the in vivo and in vitro permeabilities-estimated by <gamma>GTP and content-of the membrane of the liver microsomes appeared to reduce, suggesting an increased stability of thr microsomal membrane.

本研究旨在探讨运动对细胞膜酶通透性的影响。进行了以下三个实验：1.跑步训练：以7名久坐的男大学生为研究对象，观察了10周的运动训练（每周6次，跑5 km以上）对血浆<gamma>-谷氨酰转肽酶（<gamma>GTP）活性和浓度的影响。训练后，静息血浆<gamma>GTP浓度下降到一半，而<gamma>GTP活性没有显着变化。这表明，在训练后，GTP的前体形式<gamma>没有出现在血浆中，而“激活蛋白”出现在血浆中，可能是由于从组织如肝脏中泄漏。2.长期锻炼：15只雄性Wistar大鼠在35 ℃的水中游泳，直至力竭，体重相当于其体重的3%。在<gamma>肝细胞组分中研究了微粒体GTP。GTP活性和含量的体内和体外渗透性<gamma>通过以下公式计算：体内渗透性（%）=（微粒体外分数× 100）/（微粒体分数+微粒体分数）和体外渗透性（%）=（低渗条件）/（渗透条件）× 100<gamma>游泳后，GTP活性和含量的体内渗透性大大增加，而体外渗透性无明显变化。这表明急性运动增加了大鼠肝微粒体膜的通透性。3.游泳训练：10只雄性Wister大鼠在35 ℃的水中游泳，每天3小时，每周5次，持续10周。训练后，肝微粒体膜的体内和体外通透性（用<gamma>GTP和含量估计）均降低，表明微粒体膜的稳定性增加。