Placental transfer and tissue distribution of organic solvents----A basic study for behavioral teratological research

有机溶剂的胎盘转移和组织分布----行为畸形学研究的基础研究

• 批准号: 61570277
• 负责人: MIYAKE Hirotsugu
• 金额: $ 1.47万
• 依托单位: Sapporo Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-61570277/
• 关键词: Styrene; Placental transfer; Radioautography; Fetal distribution; Mouse(Rat); Neurobehavioral teratology; Neuro-behavioral development; 有機溶剤; スチレン; 次世代影響; 胎仔移行率; ラジオオートグラフィー; 行動奇形

The radioactivity distribution of ^<14>C-styrene was studied in the pregnant mouse using a whole body low-temperature autoradiographic technique. In unsectioned tissues studied by liquid scintilation, the concentrations of styrene and its metabolites in maternal and fetal blood and organs, and in the placenta and anniotic fluid were determined. There were considerable amounts of radioactivity in the fetuses, though the concentrations were not so high. Fetal tissue levels were almost the same as maternal brain in mice sacrificed from 1hr to 6hr after injection (report 1). In report No.2, styrene was evaluated for possible functionnal, behavioral-teratological effects in the offspring of rats exposed during gestation. Even exposure to relatively low concentrations of styrene (50 ppm) delayed some physiological developments such as the righting reflex and auditory startle reflex, in addition to causing disturbances of neuromotor coordination function and learning acquisition. Furthermore, large doses (300 ppm) lead to subtle changes in emotional behavior and increases in spontaneous activities in addition to the delay of neuro-behavioral developments such as pivoting, and to negative geotaxis.

用全身低温放射自显影技术研究了~(14)C-苯乙烯在孕鼠体内的放射性分布。在液闪研究的未切片组织中，测定了母血和胎儿血液、器官、胎盘和胎盘液中的苯乙烯及其代谢物的浓度。胎儿体内有相当数量的放射性物质，尽管浓度并不是那么高。注射后1小时至6小时处死的小鼠，胎儿组织水平与母鼠脑组织水平几乎相同(报告1)。在第2号报告中，评估了苯乙烯对妊娠期间暴露的大鼠后代可能的功能、行为和畸形学影响。即使暴露在相对较低浓度的苯乙烯(50ppm)中，也会延迟一些生理发育，如翻正反射和听觉惊吓反射，此外还会导致神经运动协调功能和学习获得的障碍。此外，大剂量(300ppm)导致情绪行为的微妙变化和自发活动的增加，以及神经行为发育的延迟，如旋转和负向地趋向性。

项目成果

岸玲子,片倉洋子,池田聰子,三宅浩次: 日本衛生学雑誌. 43. 405 (1988)

Reiko Kishi、Yoko Katakura、Satoko Ikeda、Koji Miyake：日本卫生杂志 43. 405 (1988)。

Kishi, R; Katakura Y; Okui T; Ogawa H; Ikeda T; Miyake H.: "Placental transfer and tissue distribution of C-14 styrene ----A radioautographic study in mice" Proceedings "Asia-Pacific symposium on environmental and occupational oxicology".

岸，R；