Placental transfer and tissue distribution of organic solvents----A basic study for behavioral teratological research

本文<14>用全身低温放射自显影技术研究了~（18）C-苯乙烯在孕鼠体内的放射性分布。在未切片的组织中，通过液相色谱法研究，测定了母体和胎儿血液和器官以及胎盘和羊水中苯乙烯及其代谢物的浓度。胎仔中有相当数量的放射性，尽管浓度不是很高。在注射后1小时至6小时处死的小鼠中，胎仔组织水平几乎与母体大脑相同（报告1）。在第2号报告中，评价了妊娠期间暴露于苯乙烯的大鼠后代可能的功能、行为-畸形学影响。即使暴露于相对较低浓度的苯乙烯（50 ppm）延迟一些生理发展，如翻正反射和听觉惊吓反射，除了引起神经运动协调功能和学习能力的障碍。此外，大剂量（300 ppm）导致情绪行为的微妙变化和自发活动的增加，以及神经行为发育的延迟，如旋转和负趋地性。

The radioactivity distribution of ^<14>C-styrene was studied in the pregnant mouse using a whole body low-temperature autoradiographic technique. In unsectioned tissues studied by liquid scintilation, the concentrations of styrene and its metabolites in maternal and fetal blood and organs, and in the placenta and anniotic fluid were determined. There were considerable amounts of radioactivity in the fetuses, though the concentrations were not so high. Fetal tissue levels were almost the same as maternal brain in mice sacrificed from 1hr to 6hr after injection (report 1). In report No.2, styrene was evaluated for possible functionnal, behavioral-teratological effects in the offspring of rats exposed during gestation. Even exposure to relatively low concentrations of styrene (50 ppm) delayed some physiological developments such as the righting reflex and auditory startle reflex, in addition to causing disturbances of neuromotor coordination function and learning acquisition. Furthermore, large doses (300 ppm) lead to subtle changes in emotional behavior and increases in spontaneous activities in addition to the delay of neuro-behavioral developments such as pivoting, and to negative geotaxis.