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Basical Research of Drug Resistant Mechanis in a Human Drug Resistant Ovarian Cancer cell line.

Basical Research of Drug Resistant Mechanis in a Human Drug Resistant Ovarian Cancer cell line.
人类耐药卵巢癌细胞系耐药机制的基础研究。
批准号:
61570810
负责人:
YASUDA Makoto
金额:
$1.47万
依托单位国家:
日本
项目类别:
Grant-in-Aid for General Scientific Research (C)
财政年份:
1986
资助国家:
日本
项目状态:
已结题
起止时间:
1986 至 1988

项目摘要

项目成果

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中文摘要
翻译
以人卵巢癌生殖细胞来源细胞系(JOHYL-1)为研究对象,将抗肿瘤药物阿霉素(ADM)-、顺铂(CDDP)-、碳喹(CQ)-和长春新碱(VCR)-分别以10^<-6> μ /ml的浓度培养,逐渐增加浓度,CQ、ADM和VCR的浓度从10^<-1> μ /ml增加到10^<-2> μ /ml, CDDP的浓度为10^<-1> μ /ml。对获得的每个耐药细胞群进行了生物学特性检测。1)耐药细胞在不同药物培养72小时后,IC_<50>(杯/毫升),CQ为3.2 × 10^<-3>, ADM为5.8 × 10^<-2>, VCR为1.1 × 10^<-1>, CDDP为2.5,认为耐药细胞比JOHYL-1细胞耐药28-78倍。2) cq耐药细胞倍增时间为38 h, adm耐药细胞倍增时间为58 h, vcr耐药细胞倍增时间为31 h, cdp耐药细胞倍增时间为30 h,明显大于johyl的相应值。1.3)DNA直方图研究提示cdp耐药细胞具有DNA损伤修复机制。4)对其他抗癌药物的交叉耐药研究表明,CDDP耐药细胞对ADM和CDDP耐药细胞无获得性交叉耐药证据。5)连续测定cddp耐药细胞的倍增时间、IC_<50>(马克杯/毫升)和耐药比,并测定裸鼠体内植入后这些参数的变化。结果分别为26小时、4.4 × 10^<-1>和14小时。由此可见,耐药细胞的耐药性在生物学特性上已大大降低,目前正在研究中。
英文摘要
Using a human ovarian cancer cell line of germ cell origin (JOHYL-1), Adriamycin(ADM)-, Cisplatinum(CDDP)-, Carboquon(CQ)- and Vincristine (VCR)- resistant cells were produced by culturing the cells with these anticancer drugs at 10^<-6> mu/ml initially, with the concentration boing then increased stepwise by 10^<-1> mug/ml up to 10^<-2> mug/ml for CQ, ADM and VCR and 10^<-1> mug/ml for CDDP. Each of the resistant cells population thus obtained were examined for their biological properties.1) The drug-resistant cells, when exposed in culture to respective drugs for 72 hours, gave IC_<50> (mug/ml) of 3.2 x 10^<-3> for CQ, 5.8 x10^<-2> for ADM, 1.1 x 10^<-1> for VCR and 2.5 for CDDP, thus being thought to 28-78 times more resistant than JOHYL-1 cells.2) The doubling time was 38 hes for CQ-resistant cells, 58 hrs for ADM-resistant cells, 31 hrs for VCR-resistant cells and 30 hrs for CDDP-resistant cells, thus being definitely longer than the corresponding value for JOHYL-1.3) Study of DNA histograms suggested that CDDP-resistant cells are provided with a DNA damage-respairing mechanism.4) Study of cross resistance to other anticancer drugs showed that CDDP-resistant cells to ADM and ADR-resistant cells to CDDP were no evidence of acquired cross resistance.5) CDDP-resistant cells were measured serially for the doubling time, IC_<50> (mug/ml) and resistance ratio and also determined for changes in these parameter after being implanted in nude mice. The obtained results were 26 hrs, 4.4 x 10^<-1> and 14, respectively. It became thus obvious that the drug resistance of CDDP-resistant cells were greatly diminished in biologic property is currently being investigated.
期刊论文(8)
专著(0)
科研奖励(0)
会议论文
安田允: 最新薬物療法. 44. 1113-1115 (1986)
安田胜:最新药物治疗。44. 1113-1115 (1986)
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通讯作者:
礒西成治: 日本臨床細胞学会. 25. 1017-1024 (1986)
N. Isonishi:日本临床细胞学学会。25. 1017-1024 (1986)
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芳岡三伊: 日本産科婦人科学会雑誌. 38. 1685-1691 (1986)
Yoshioka, M.:日本妇产科学会杂志 38. 1685-1691 (1986)。
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堂園 晴彦: 日本産科婦人科学会雑誌. 39. 1968-1972 (1987)
Haruhiko Dozono:日本妇产科学会杂志 39。1968-1972(1987)。
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8
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