Functional Solvent Design for Separation of Bioproducts

根据生物技术的发展、充足的分离技术对生物产品的要求是非常严格的。在几种分离技术中，一种净化方法，将生物产物划分为两阶段系统似乎是一种被授予的方法，因为该方法将提供一种温和的条件。为了设计最佳溶剂条件，基本需要的是双阶段系统的相位行为和生物产物的分区协同作用的知识。在目前的研究中，对右旋糖酐-聚乙烯糖醇-水系统的液体平衡物在20 ° C时进行了测量。目前的液体等价物与文学价值观有关。此外，它还发现了这样一个相位系统的相位行为，即与弗洛里-哈金斯方程相关。使用的代理人是Pharmacia Co. Ltd.的dextran。（批号03375， Mn=24200， T40）和Wako Chemicals，Ltd.供应的聚乙基糖醇。（Lot No.AWM2336， Mn=3000， PEG4000）来自Wako Chemicals，Ltd.的蒸馏水被用作纯净水，Wako Chemicals，Ltd.供应的淀粉酶的分区作用。（Bacillus Subtiles， Lot No.PDGO524）到上面的两个阶段系统是可测量的。从目前的数据中，找到了-淀粉酶主要分布到底部阶段（右旋丰富阶段）。这一部分的协同作用与弗洛里-希金斯等式有关。已经找到了一个相互作用的参数，取决于分子的重量。

According to the development of biotechnology, sufficient separation techniques for bioproducts are strongly required. Among several separation techniques, a purification method due to the partition of bioproducts to aqueous two-phase system seems to be one of promising methods because the aqueous system provides a mild condition. To design the optimum solvent condition, the knowledge of phase behavior of the aqueous two-phase system and the partition coefficient of bioproducts is essentially needed.In the present study, the liquid-liquid equilibria of the dextran-polyethylene glycol- water system were measured at 20゜C. The present liquid-liquid equilibria coincide with the literature values. Further, it is found that the phase behavior of the aqueous two-phase system can be correlated by the Flory-Huggins equation. The reagents used are dextran from Pharmacia Co.Ltd.(Lot No.03375, Mn=24200, T40) and polyethylene glycol supplied by Wako Chemicals, Ltd.(Lot No.AWM2336, Mn=3000, PEG4000). Distilled water from Wako Chemicals, Ltd. was used as pure water.The partition coefficients of -amylase supplied by Wako Chemicals, Ltd.(Bacillus Subtiles, Lot No.PDGO524) to the above aqueous two-phase system were measured. From the present data, it is found that -amylase is distributed mainly to the bottom phase (dextran rich phase). This partition coefficients were correlated by the Flory-Higgins equation. The interaction parameters are found to depend on the molecular weight.