Neuronal activity and retrograde intra-axonal transport.

神经元活动和逆行轴突内运输。

• 批准号: 62570069
• 负责人: KOIKE Hiroyuki
• 金额: $ 1.28万
• 依托单位: Tokyo Metropolitan Institute for Neurosciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62570069/
• 关键词: 逆行性軸索内輸送; 開口放出; 急速凍結法; シビレエイ

It is generally accepted that synaptic vesicles in the pre-synaptic nerve terminal fuse with the presynaptic terminal membrane and release the transmitter. The resulted extra terminal membrane will be uptaken into the terminal by clathrin mediated pinocytosis. Therefore, the synaptic activation will increase the number of coated vesicles in the terminal and also may increase the number of retrogradely transporting vesicles along the axon. We have tried to clarify the assumption using cholinergic nerve terminals of electric organ of Torpedo and Narke. The terminal region was quick-frozen while stimulating the clectric nerve. The replica examined by electron microscopy showed many synaptic vesicles in the terminal, but no significant differences between stimulated one and control. We also examined the retrograde axonal transport upon nerve stimulations. The movement of particles along the axon was analyzed by computer enhanced video image of the interference contrast microscopy of the fine branches of the electric nerve. But, their movements was not modified by stimulation of the nerve.

一般认为，突触前神经末梢的突触囊泡与突触前终膜融合并释放递质。所产生的额外终膜将通过网格蛋白介导的胞饮作用被摄取到终末。因此，突触激活将增加终末的被膜囊泡的数量，也可能增加轴突沿着逆行运输囊泡的数量。我们试图用电鳐和家鼠的电器官的胆碱能神经末梢来阐明这一假设。在刺激电神经的同时，将末端区域快速冷冻。电子显微镜检查的复制品显示许多突触囊泡在终端，但刺激一个和对照组之间没有显着差异。我们还研究了神经刺激后的逆行轴突运输。通过计算机增强的电神经细分支的干涉对比显微镜视频图像分析颗粒沿着轴突的运动。但是，它们的运动并没有被神经刺激所改变。