Pharmacological studies on the sympathetic nerve-effector mechanisms in the blood vessel.

1.我们先前的研究提出了一项建议，即在过渡神经刺激（TNS）方面开发出比后分泌（NE）和海绵（TNS）的前超敏感性缓慢且迟于后分泌物（Tsuru & Uematsu， Japan）。J. Pharmacol。1986年10月27日至282日）这一建议是根据目前的研究提出的。在厌食症下，单地前anglionic纤维（SCG）被排除在外，而殖民地方面则被排除在控制之外。去中心化和控制arteries在去中心化操作之后隔离1、2、4和8周。每个arterial preparation was incubated with ^3 H-NE10 ^- M for 1 hr at 37 c。内部压力中的变化被监测，而完美的变化被一个片段收集器收集了每2分钟。在^3 H-NE溢出从TNS通过液体闪烁计数器测量的超饱和和超饱和的artery。^3 H-NE溢出在8-weeks decentrali中的低频率在8周的布局中显著增加 ... More 2.如果将zation与另一个受控的artery进行比较，那么在相对短期的decentralization中就没有显著的变化。^3 H-Norepinephrine uptake experiment was made after incubation with ^3 H-NE10 ^\-8> M for 1 hr at 37 c in the absence and in the presence of cocaine 10^\-4> M. ^3 H-NE在8周的布局和控制之间没有明显的差异。植物中的儿茶素和SCG被修改后的放射性酶薄层色谱法检测。多巴胺、NE和肾上腺素在两种植物之间的含量和8周的分散化和控制的SCG中没有明显的差异，而被拒绝的植物中的儿茶素含量被显著地减少。目前的研究证实，我们先前的建议是，神经发射器释放中的增量对TNS的超敏感性的、对TNS的超敏感性作出反应，并提出的建议是，在刺激-保密组合中的变化可能会受到影响，但机制仍然存在。Less（低）

1. Our previous studies have suggested that the prejunctional supersensitivity to transmural nerve stimulation (TNS) develops slowly and later than the postjunctional one to exogenous norepinephrine (NE) and potassium (Tsuru ＆ Uematsu, Japan. J. Pharmacol. 40: 273-282, 1986). This suggestion was pursued in the present study.2. Under anesthesia, unilateral preganglionic fiber to the superior cervical ganglion (SCG) was excised and the contralateral side was left intact as the control. Decentralized and control arteries were isolated 1, 2, 4 and 8 weeks after decentralization operation.3. Each arterial preparation was incubated with ^3H-NE 10^- M for 1 hr at 37゜c. Changes in intraluminal pressure was monitored and the perfusate was collected every 2 min by a fraction collector. Increase in ^3H-NE overflow from perfused and superfused artery by TNS was measured by a liquid scintillation counter. ^3H-NE overflow was remarkably increased at low frequenies in the artery of 8-weeks decentrali … More zation as compared with the contralateral control artery, while there was no significant change in the relatively short term decentralization.4. ^3H-Norepinephrine uptake experiment was made after incubation with ^3H-NE 10^<-8> M for 1 hr at 37゜c in the absence and in the presence of cocaine 10^<-4> M. There was no significant difference in ^3H-NE uptake between the arteries of 8-weeks decentralization and control.5. Catecholamines in the artery and SCG were assayed by the modified radioenzymatic thin layer chromatographic method. There was no significant difference in the content of dopamine, NE and epinephrine between the both arteries and SCG of 8-weeks decentralization and control, whereas catecholamine content in the denervated artery was remarkably decreased.6. The present study confirms our previous suggestion that the increment in neurotransmitter release is responsible for supersensitivity of the chronically decentralized rabbit ear artery to TNS, and suggests that change in stimulus-secretion coupling may be involved although the mechanism remains to be resolved. Less