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Pharmacological studies on the sympathetic nerve-effector mechanisms in the blood vessel.

血管中交感神经效应机制的药理学研究。

基本信息

批准号：
62570087
负责人：
TSURU Hiromichi
金额：
$ 1.34万
依托单位：
HIROSHIMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1988
项目状态：
已结题

项目摘要

1. Our previous studies have suggested that the prejunctional supersensitivity to transmural nerve stimulation (TNS) develops slowly and later than the postjunctional one to exogenous norepinephrine (NE) and potassium (Tsuru ＆ Uematsu, Japan. J. Pharmacol. 40: 273-282, 1986). This suggestion was pursued in the present study.2. Under anesthesia, unilateral preganglionic fiber to the superior cervical ganglion (SCG) was excised and the contralateral side was left intact as the control. Decentralized and control arteries were isolated 1, 2, 4 and 8 weeks after decentralization operation.3. Each arterial preparation was incubated with ^3H-NE 10^- M for 1 hr at 37゜c. Changes in intraluminal pressure was monitored and the perfusate was collected every 2 min by a fraction collector. Increase in ^3H-NE overflow from perfused and superfused artery by TNS was measured by a liquid scintillation counter. ^3H-NE overflow was remarkably increased at low frequenies in the artery of 8-weeks decentrali … More zation as compared with the contralateral control artery, while there was no significant change in the relatively short term decentralization.4. ^3H-Norepinephrine uptake experiment was made after incubation with ^3H-NE 10^<-8> M for 1 hr at 37゜c in the absence and in the presence of cocaine 10^<-4> M. There was no significant difference in ^3H-NE uptake between the arteries of 8-weeks decentralization and control.5. Catecholamines in the artery and SCG were assayed by the modified radioenzymatic thin layer chromatographic method. There was no significant difference in the content of dopamine, NE and epinephrine between the both arteries and SCG of 8-weeks decentralization and control, whereas catecholamine content in the denervated artery was remarkably decreased.6. The present study confirms our previous suggestion that the increment in neurotransmitter release is responsible for supersensitivity of the chronically decentralized rabbit ear artery to TNS, and suggests that change in stimulus-secretion coupling may be involved although the mechanism remains to be resolved. Less

1.我们以前的研究表明，对透壁神经刺激（TNS）的连接前超敏性比对外源性去甲肾上腺素（NE）和钾的连接后超敏性发展得慢且晚（Tsuru & Uematsu，Japan. J. Pharmacol. 40：273-282，1986）。这项建议在本研究中得到了贯彻。麻醉下，切除一侧颈上级神经节（SCG）的节前纤维，保留对侧作为对照。去中心化手术后1、2、4、8周分离去中心化动脉和对照动脉。将每份动脉标本与10^- M ^3 H-NE在37 ℃下孵育1小时。监测管腔内压力的变化，并通过馏分收集器每2分钟收集一次灌注液。通过液体闪烁计数器测量TNS灌注和灌流动脉中^3 H-NE溢出量的增加。在8周的偏侧颈动脉中，低频时^3 H-NE溢出明显增加。 ...更多信息与对侧对照动脉相比，双侧颈总动脉的偏心度无明显变化，而相对短期的偏心度无明显变化.在<-8>不存在和存在10 μ M可卡因的情况下，在37 ℃下与10 μ M ^3 H-去甲肾上腺素孵育1小时后<-4>，进行^3 H-去甲肾上腺素摄取实验。去中心化8周组与对照组动脉的^3 H-NE摄取量无显著性差异.用改良的放射酶薄层色谱法测定动脉和颈总动脉中的儿茶酚胺。去神经8周组和对照组的多巴胺、去甲肾上腺素和肾上腺素含量与对照组相比无显著性差异，而去神经组的儿茶酚胺含量显著降低.本研究证实了我们以前的建议，即神经递质释放的增加是负责的慢性分散兔耳动脉TNS的超敏感性，并建议刺激分泌耦合的变化可能参与，但其机制仍有待解决。少