Study on growth and differentiation of human eosinophils

用鲁米诺依赖性化学发光法研究了嗜酸性粒细胞EoL-1在磷酯（TPA）和n -甲酰基肽（fMLP）刺激下的呼吸爆发。干扰素- (IFN-)治疗EoL-1 5天，可增强tpa诱导的CL反应。IFN- A也有类似的效果。肿瘤坏死因子（Tumor necrosis factor， TNF）也增强了EOL-1的TPAinducible CL反应，并且对EOL-1细胞的生长有一定的抑制作用。另一方面，白细胞介素5 （IL-5）或粒细胞-巨噬细胞集落刺激因子（GM-CSF）对tpa诱导的EoL-1的CL无增强作用。而IL-5对IFN-的作用略有增强。此外，IL-5和GM-CSF对EoL-1均有轻微的促生长作用。用IFN-、- A或TNf预处理的EoL-1也能够对fMLP产生CL反应，IFN-和TNf再次协同作用。用IFN-、- A或TNF处理的EoL-1有丰富的细胞质，但只有TNF增加了具有明显嗜酸性颗粒的细胞。IFN-增强了TNF的细胞学作用。进一步证明，与IFN-和TNF同时处理EoL-1强烈增加了phobol di酯的结合位点，并显著诱导了fMLP受体的表面表达。综上所述，EoL-1似乎为研究人类嗜酸性粒细胞的个体发生和功能及其产物来源提供了一个有价值的实验模型。

Human eosinophilic cell line EoL-1 was studied by using luminol-dependent chemiluminescence for respiratory burst upon stimulation with phorbol ester (TPA) and N-formylpeptide (fMLP). Treatment of EoL-1 with interferon- (IFN- ) for 5 days strongly enhanced TPA-inducible CL response. IFN- A had a similar effect. Tumor necrosis factor (TNF) also enhanced TPAinducible CL response of EOL-1 and, furthermore, was quite inhiditory to EoL-1 cell growth. On the other hand, interleukin 5 (IL-5) or granulocyte-macrophage colony stimulating factor (GM-CSF) had no augmenting effect on TPA-inducible CL in EoL-1. IL-5, however, slightly enhanced the effect of IFN- . Furthermore, both IL-5 and GM-CSF showed slight growth-promoting effects on EoL-1. EoL-1 pretreated with IFN- , - A or TNf also became capable of producing CL response to fMLP, and IFN- and TNF were again synergistic.EoL-1 treated with IFN- , - A, or TNF had abundant cytoplasm, but only TNF increased cells having distinct eosinophilic granules. IFN- enhanced this cytological effect of TNF. It was further demonstrated that the simultaneous treatment of EoL-1 with IFN- and TNF strongly increased the binding sites for phorbol diesters and also dramatically induced the surface expression of fMLP receptors. Taken together, EoL-1 seems to provide a valuable experimental model for the study of the ontogeny and the function of human eosinophils and as a source of their products.