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老化に伴う臓器局在性自己免疫疾患の発症機序に関する実験的研究

衰老相关器官局限性自身免疫性疾病发病机制的实验研究

基本信息

批准号：
63570171
负责人：
林良夫
金额：
$ 1.28万
依托单位：
Tokyo Metropolitan Institute of Gerontology
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至无数据
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63570171/
关键词：
老化自己免疫 T細胞免疫組織化学マウス

项目摘要

NZB/W,MRL/Lpr,SL/Niなどの系統的自己免疫疾患マウスにしばしば唾液腺炎、腎血管炎などの臓器局在性自己免疫疾患が随伴することはよく知られている。老化に伴う免疫異常がある種の臓器局在性自己免疫疾患の発症に関与している可能性を明らかにすることを目的とし、加齢の進行したC57BL/6マウスの全身諸臓器を詳細に検索した。[材料と方法]老人研飼育老化マウスC57BL/BNCrjを使用。月齢3ヶ月・24ヶ月までの雌雄126匹につき、通常の光顕的観察の他、凍結切片を用いた免疫組織化学的観察(ABC法)を実施した浸潤細胞亜群の解析を行った。また、老化マウスに於ける血中自己抗体の検出を間接螢光抗体法にて実施した。[結果]老化マウスに多臓器に及ぶ自己免疫性病変の発症が認められた。即ち、雌雄マウスとも唾液腺炎、腎血管周囲炎、膵ラ氏島炎、肺血管周囲炎、肝内胆管炎の自然発症をみた。巣状リンパ球浸潤(臓器炎)は12ヶ月、18ヶ月、24ヶ月と加齢の進行とともに頻度、程度とも増強する傾向がみられた。免疫組織化学的に臓器浸潤細胞の表現型を解析した結果、Thy-1.2陽性T細胞が主体で(80%)、その亜群としてはL3T4陽性リンパ球が優位(70%)でLyt-2陽性リンパ球は10%前後であった。またlgs陽性細胞は4%-9%に過ぎなかった。老化マウス血中の自己抗体を間接螢光抗体法にて検索した結果、老化マウスに高頻度に検出された。その局在は、唾液腺導管上皮、腎尿細管上皮・基底膜、膵ラ氏島細胞、肺細気管支上皮、肝細胆管上皮であった。[考察]老化の進行したC57BL/6マウスに高頻度に多臓器にわたる自己免疫性病変化の自然発症をみたとこは、ある種の臓器局在性自己免疫疾患は老化に伴う免疫異常を基盤にして発症する可能性が示唆された。

The self-immune diseases of the NZB/W,MRL/Lpr,SL/Ni immune system, such as sialitis and vasculitis, are associated with sexual autoimmune diseases. Aging is associated with immune disease. The immune system is often used in the diagnosis and treatment of self-immune diseases. The possibility of the disease is different. The purpose of this study is to improve the quality of the disease. In addition, the C57BL/6 is used to treat the disease. [materials and methods] for the elderly to study, nurture and aging, and to use C57BL/BNCrj. On the 24th month of the third month, the male and female of the lunar month were 126. the light was usually observed, and the sections were examined by immunohistochemistry (ABC method). The cell population was analyzed. The antibodies were detected in the blood of patients with aging and were exposed to light antibody method. [results] the aging patients had multiple devices and the symptoms of self-immune venereal diseases. That is, male and female, male and female, sialiditis, perivascular inflammation, pulmonary perivascular inflammation, intrahepatic cholangitis, and so on. After 12 months, 18 months and 24 months, the temperature and the degree of temperature and intensity were increased. The results of immunocytochemistry analysis showed that the body of Thy-1.2-specific T-cell was 80%, and that of Lyt-2-positive T-cell was 10%. 4% of lgs sexual interviewees are not eligible for health care. The autoantibodies in the blood of aging mice were detected by photoantibody method, and the results of light antibody test and aging test were detected. The epithelium of salivary gland, the basement membrane of urinary duct, the epithelium of the branch of pulmonary duct, the epithelium of bile duct of liver. [investigation] the possibility of aging in patients with sexual autoimmune diseases is indicated by the possibility of aging of sexual autoimmune diseases associated with immune diseases. [investigation] the possibility of aging in patients with sexual autoimmune diseases is indicated by the possibility of C57BL/6 aging, autoimmune diseases, self-immune STDs, natural symptoms, natural symptoms, and sexual diseases.