Immuno-pathological studies in IgA nephropathy : The role of virus for etiological and progressive factors

为了产生伊加肾病的实验模型，对接种病毒的小鼠进行了组织学和病毒学研究。首先，以1-28天的间隔静脉内接种小鼠两次，用Cox.B4病毒纯化。在第二次接种后36小时从每只小鼠获得肾脏，并检查组织学变化如系膜沉积。在间隔14天或更长时间接种两次的小鼠中，系膜沉积物明显，表明以足够长的间隔重复抗原刺激对于产生系膜沉积物是必要的。另一系列4周龄小鼠以4周间隔静脉内（i. v.组）或腹膜内（i. p.组）接种Cox.B4病毒4-8次。在最后一次接种后一个月从每只小鼠获得肾脏，并检查组织学变化。在静脉注射组，光镜检查显示PAS阳性沉积物在系膜后4 ...更多信息 接种。接种6次或6次以上后，沉积物更加明显，并伴有袢壁不均匀肥大和新月体形成。通过免疫荧光法，IgG在4或5次接种后获得的动物中染色最深，而伊加和IgG在6次或更多次接种后的一些动物中染色程度相同。所有接种者均为病毒抗原阳性，染色强度随接种次数的增加而增加。电子显微镜检查显示沉积在上皮下和内皮下军团。在腹腔注射组中，光学显微镜显示接种4次或更多次后系膜中PAS阳性沉积物。接种6次或6次以上后，基底膜不均匀肥大，但未形成新月体。通过免疫荧光，IgG，伊加和IgM在系膜中染色较深，与对照组相比，伊加总是染色为主。病毒抗原均为阳性。电子显微镜检查结果与静脉注射组相似。因此，证明了在小鼠中间歇性腹膜内接种Cox.B4病毒产生与人类伊加肾病非常相似的肾脏病变。少

In order to produce an experimental model of IgA nephropathy, a histologiral and virological study of mice inoculated with a virus was carried out. First, mice were intravenously inoculated twice at an interval of 1-28 days purified with Cox.B4 virus. The kidneys were obtained from each mouse 36 hours after the second inoculation, and examined for histological changes such as mesangial deposits. Mesangial deposits were marked in mice given two inoculation at an interval of 14 days or more, indicating that antigen stimulations repeated at a sufficiently long interval are necessary for producing mesangial deposits. Another series of 4-week-old mice were inoculated with Cox.B4 virus 4-8 times at 4-week intervals either intravenously (i.v. group) or intraperitoneally (i.p. group). The kidneys were obtained from each mice one month after the final inoculation, and examined for histological changes. In the i.v. group, light microscopy revealed PAS- positive deposits in the mesangium after 4 … More inoculations. The deposits were more marked after 6 or more inoculations, accompanied by uneven hypertrophy of the loop wall and formation of crescents. By immunofluorescence, IgG was most deeply stained in speciments obtained after 4 or 5 inoculations, whereas IgA and IgG were stained to the same degree in some speciments after 6 or more inoculations. All speciments were positive for virus-Ag, the intensity of staining being increased in proportion to the number of inoculations. Electron microscopy disclosed deposits in subepithelial and subendothelial legions. In the i.p. group, light microscopy showed PAS-positive deposits in the mesangium after 4 or more inoculations. After 6 or more inoculations, there was uneven hypertrophy of the basement membrane, but no formation of crescents. By immunofluorescence, IgG, IgA and IgM were deeply stained in the mesangium in comparison with controls; IgA was always stained predominantly. Virus-Ag was positive in all speciments. Electron microscopic findings were similar to those in the i.v. group. It was thus demonstrated that intermittent intraperitoneal inoculation with Cox.B4 virus in mice produces renal lesions closely resembling those in human IgA nephropathy. Less