Evidences of a novel splicing mechanism in DMD gene

DMD 基因中新型剪接机制的证据

• 批准号: 22710192
• 负责人: SUZUKI Hitoshi
• 金额: $ 2.5万
• 依托单位: Japan Advanced Institute of Science and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22710192/
• 关键词: RNA; スプライシング; 疾患関連遺伝子; エキソンスキップ治療

Lariat intron is a by-product generated by pre-mRNA splicing. In this study, I investigated a possibility that an ends-connecting intron could be produced in vivo. The results suggested that the ends-connecting introns were widely detected. There was likely to be no tissue dependence or intron dependence. Besides, it was suggested that RNase R-resistant RNA contained not only intron RNAs, but also many unknown RNAs located in the first exon regions.

套索内含子是前体 mRNA 剪接产生的副产物。在这项研究中，我研究了在体内产生末端连接内含子的可能性。结果表明末端连接内含子被广泛检测到。可能不存在组织依赖性或内含子依赖性。此外，有人认为RNase R抗性RNA不仅含有内含子RNA，还含有许多位于第一外显子区域的未知RNA。

项目成果

Functional gene expression analysis of tissue-specific is forms of Mef2c

组织特异性功能基因表达分析是 Mef2c 的形式

• 发表时间: 2012
• 期刊: Cell. Mol
• 作者: Sekiyama;Y.;Suzuki;H.;and Tsukahara;T.
• 通讯作者: T.

Re-splicing of mature mRNA in cancer cells promotes activation of distant weak alternative splice sites.

• DOI: 10.1093/nar/gks520
• 发表时间: 2012-09
• 期刊: Nucleic acids research
• 影响因子: 14.9
• 作者: Kameyama T;Suzuki H;Mayeda A
• 通讯作者: Mayeda A

P19細胞の神経分化における選択的スプライシングの網羅的解析と経路網探索

P19细胞神经元分化中选择性剪接和路径网络探索的综合分析

• 发表时间: 2010
• 作者: Adachi S;Nishida N;et al.;他15名;鈴木仁
• 通讯作者: 鈴木仁

Alternative splicing produces structural and functional changes in CUGBP2.

• DOI: 10.1186/1471-2091-13-6
• 发表时间: 2012-03-20
• 期刊: BMC biochemistry
• 作者: Suzuki H;Takeuchi M;Sugiyama A;Alam AK;Vu LT;Sekiyama Y;Dam HC;Ohki SY;Tsukahara T
• 通讯作者: Tsukahara T

Functional Gene Expression Analysis of Tissue-Specific Isoforms of Mef2c

• DOI: 10.1007/s10571-011-9743-9
• 发表时间: 2011
• 期刊: Cellular and Molecular Neurobiology
• 影响因子: 4
• 作者: Yoshiharu Sekiyama;H. Suzuki;T. Tsukahara