Assssing evolutionary episodes of genetic hybridization in small mammals using the population haplotype structure analysis

在这项研究中，我们的目的是开发一种新的方法，以更好地了解空间和时间方面的次级接触，其中两个不同的血统不同的地理群体混合，重点是重组信号沿着一定的染色体部分，如10 kb，200 kb，1 Mb，和5 Mb。例如，在200 kb片段的情况下，我们确定了以20-30 kb间隔分开的8个基因区域（~500 bp）中的核苷酸序列，并评估了其单倍型结构。这些方法使我们能够探测到不同时间尺度的次级接触事件，例如几百年前和几十万年前。该方法有待进一步改进，以期建立一种在分子植物地理学研究中充分利用重组事件相关遗传信息的方法。

In this study, we aimed to develop a new method to better understand spacial and temporal aspects for the secondary contact in which two distinct lineages of different geographic groups are mingled, focusing on recombination signals along a certain chromosome portion, such as 10 kb, 200 kb, 1 Mb, and 5 Mb. In a case of 200 kb segment, for example, we determined nucleotide sequences in eight gene region (~500 bp) being separated at 20-30 kb interval and assessed its haplotype structures. These methods allowed us to detect the secondary contact events of different time scales, such as several hundred years before present and several hundred thousand years ago. Further efforts would be needed to improve the method, aiming at establishment of ways to fully utilize the genetic information related to recombination events in molecular phylogeographic study.