Basic Research on Biological Monitoring of Industrial Chemicals in Japan

1. 采用高效液相色谱法（HPLC），以配对离子为流动相，同时测定肌酐和氯苯代谢物4-氯儿茶酚和对氯酚的含量。有机溶剂（甲苯、二甲苯、氯苯和三氯乙烯）在暴露结束前1、2、3和4小时的尿液代谢物浓度依次递减，表明最后一次排尿与暴露结束时间的间隔对尿液代谢物浓度的测定具有重要意义。为了储存尿液代谢物，将一张滤纸浸入接触甲苯的工人的尿液中并晾干。将尿中马尿酸和肌酐溶解于50%的甲醇中，用高效液相色谱法测定其浓度。甲醇提取物中马尿酸与肌酐的比值与原始尿液中马尿酸与肌酐的比值基本一致。为了评价尿中代谢物浓度测定的校正方法，测定了空气中甲苯浓度与尿中实际或校正的马尿酸浓度之间的相关系数。空气中甲苯与肌酐校正后的尿中马尿酸浓度的相关系数高于比重或实际浓度校正后的相关系数。

1. Simultaneous determination of creatinine and chlorobenzene metabolites, 4-chlorocatechol and p-chlorophenol, by High Performance Liquid Chromatography (HPLC) using paired ion as a mobile phase was conducted.2. Concentrations of urinary metabolites of organic solvents (toluene, mxylene, chlorobenzene and trichloroethylene) in 1, 2, 3 and 4 hours before the end of exposure were in the descending order, indicating that the interval between the last urination and the end time of exposure plays an importantrole for determining urinary concentration of the urinary metabolites.3. For a purpose of storage of urinary metabolites, a piece of filter paper was dipped in urine of workers exposed to toluene and dried. Urinary hippuric acid and creatinine in the paper were dissolved into 50% methanol and their concentrations were determined by HPLC. The ratio of hippuric acid to creatinine concentration in methanol extract and that in original urine was similar to each other.4. In an attempt to evaluate the correction methods for determination of metabolite concentration in urine, the correlation coefficient between the toluene concentration in the air and the actual or corrected concentration of hippuric acid in the urine was determined. The correlation coefficients between the toluene in the air and the hippuric acid concentration in the urine corrected for creatinine was higher than that corrected for specific gravity or actual concentration.