Basic Research on Biological Monitoring of Industrial Chemicals in Japan

日本工业化学品生物监测基础研究

• 批准号: 01570313
• 负责人: OGATA Masana
• 金额: $ 1.34万
• 依托单位: Department of Public Health, Okayama University Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01570313/
• 关键词: biological monitoring; normal value; organic organic; heavy metal; air concentration; ary concentration; urinary concentration; 06environmental concentration; クレアチニン補正法; 生物学的モニタリング; クレアチニン補正; 比重補正; 有機溶剤健康診断; イオン対クロマトフラフィ-; ろ紙保存法

1. Simultaneous determination of creatinine and chlorobenzene metabolites, 4-chlorocatechol and p-chlorophenol, by High Performance Liquid Chromatography (HPLC) using paired ion as a mobile phase was conducted.2. Concentrations of urinary metabolites of organic solvents (toluene, mxylene, chlorobenzene and trichloroethylene) in 1, 2, 3 and 4 hours before the end of exposure were in the descending order, indicating that the interval between the last urination and the end time of exposure plays an importantrole for determining urinary concentration of the urinary metabolites.3. For a purpose of storage of urinary metabolites, a piece of filter paper was dipped in urine of workers exposed to toluene and dried. Urinary hippuric acid and creatinine in the paper were dissolved into 50% methanol and their concentrations were determined by HPLC. The ratio of hippuric acid to creatinine concentration in methanol extract and that in original urine was similar to each other.4. In an attempt to evaluate the correction methods for determination of metabolite concentration in urine, the correlation coefficient between the toluene concentration in the air and the actual or corrected concentration of hippuric acid in the urine was determined. The correlation coefficients between the toluene in the air and the hippuric acid concentration in the urine corrected for creatinine was higher than that corrected for specific gravity or actual concentration.

1.建立了以对离子为流动相的高效液相色谱同时测定肌酐和氯苯代谢物4-氯邻苯二酚和对氯苯酚的方法。接触结束前1、2、3、4h尿中有机溶剂(甲苯、二甲苯、氯苯、三氯乙烯)的尿代谢物浓度由高到低依次为：末次排尿至接触结束的时间间隔对尿液代谢产物浓度的测定起着重要的控制作用。为了储存尿液代谢物，将一张滤纸浸泡在接触甲苯的工人的尿液中，并将其晾干。将尿液中的马尿酸和肌酐溶于50%甲醇中，用高效液相色谱法测定其浓度。甲醇提取物和原尿中马尿酸/肌酐浓度的比值相近。为了评价测定尿液中代谢物浓度的校正方法，测定了空气中甲苯浓度与尿中马尿酸实际浓度和校正浓度之间的相关系数。空气中甲苯浓度与尿中马尿酸浓度之间的相关系数，经肌酐校正后高于按比重或实际浓度校正后的数值。

项目成果

Masana Ogata,Tomoko Iwamoto: "Enzymatic assay of formic acid and gas chromatography of for urinary biological monitoring of exposure to methanol." International Archives of Occupational and Environmental Health. 62. 227-232 (1990)

Masana Ogata、Tomoko Iwamoto：“甲酸酶法测定和气相色谱法用于尿液中甲醇暴露的生物监测。”

Masana Ogata;Tomoko Iwamoto;Toyohiro Taguchi: "Urinary 2,5ーhexanedione assay involving its conversion to 2,5ーdimethylpyrrole.(in press)" (1991)

Masana Ogata；Tomoko Iwamoto；Toyohiro Taguchi：“涉及将其转化为 2,5-二甲基吡咯的尿液 2,5-己二酮测定。（正在印刷中）”（1991 年）

M. Ogata, T. Iwamoto and T. Taguchi: "Determination of urinary 2, 5-hexanedione by its conversion to 2, 5-dimethylpyrrole." Ind. Health,. 28. 125-131 (1990)

M. Ogata、T. Iwamoto 和 T. Taguchi：“通过将尿 2, 5-己二酮转化为 2, 5-二甲基吡咯来测定尿中 2, 5-己二酮。”

M. Ogata: "Biological monitoring-theory and practice-." Shinohara. 186 (1991)

M. Ogata：“生物监测-理论与实践-”。

田口 豊郁: "有機溶剤の生物学的モニタリングのための尿試料の保存と尿中度の補正について" 岡山医学会雑誌. 102. 99-111 (1990)

Toyoiku Taguchi：“有机溶剂生物监测中尿液样本的保存和尿液浓度的校正”冈山医学会杂志 102. 99-111 (1990)。